LOCUS       sequence-451844-        9820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to 
            measure realtime changes in FRET reflecting fluctuations in cAMP 
            levels in living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Zanetti G, Klarenbeek JB, Jalink K
  TITLE     Cytosolic-enhanced dark Epac-based FRET sensors allow for 
            intracellular cAMP detection in live cells via FLIM.
  JOURNAL   FEBS Lett. 2024 Dec 31. doi: 10.1002/1873-3468.15093.
  PUBMED    39737677
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2024 Dec 31. doi: 10.1002/1873-3468.15093."
COMMENT     Sequence Label: Epac-S-H332
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..546)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2932..3666
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             3724..4458
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(4485..4503)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4486..4503)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4523..4540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4529..4753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4799..5227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4886..4905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5096..5117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5236..5256)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5241..5570
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5421..5556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5483..5502
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5637..6431
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5691..5710)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6301..6320
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6605..6726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6642..6661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6696..6715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6775..6791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6775..6791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6788..6810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6799..6815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6823..6829,6830..6847,6848..6853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6868..6889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7006..7023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7177..7765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7257..7276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7936..8727,8728..8796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8559..8578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8797..8901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8976..8995)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9167..9546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9547..9750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9701..9721
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9795..9814
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9795..9813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actactttag cgacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc ccgatatcag
      721 cccgtgggaa ctcatgagat ggaggaggag ttggccgaag ctgtggccct gctctcccag
      781 cgggggcctg acgccctgct cactgtggca cttcgaaagc ccccaggtca gcgcacggat
      841 gaagagctgg acctcatctt tgaggagctg ctgcacatca aggctgtggc ccacctctcc
      901 aactcggtga agcgagaatt agcggctgtt ctgctctttg aaccacacag caaggcaggg
      961 accgtgttgt tcagccaggg ggacaagggc acttcgtggt acattatctg gaagggatct
     1021 gtcaacgtgg tgacccatgg caaggggctg gtgaccaccc tgcatgaggg agatgatttt
     1081 ggacagctgg ctctggtgaa tgatgcaccc cgggcagcca ccatcatcct gcgagaagac
     1141 aactgtcatt tcctgcgtgt ggacaagcag gacttcaacc gtatcatcaa ggatgtggag
     1201 gcaaagacca tgcggctgga agaacatggc aaagtggtgc tggtgctgga gagagcctct
     1261 cagggcgccg gcccttcccg acccccaacc ccaggcagga accggtatac agtgatgtct
     1321 ggcaccccag agaagatcct agagcttctg ttggaggcca tgggaccaga ttccagtgct
     1381 catgacccaa cagagacatt cctcagcgac ttcctcctga cccacagggt cttcatgccc
     1441 agcgcccaac tctgcgctgc ccttctgcac cacttccatg tggagcctgc gggtggcagc
     1501 gagcaggagc gcagcaccta cgtctgcaac aagaggcagc agatcttgcg gctggtcagc
     1561 cagtgggtgg ccctgtatgg ctccatgctc cacactgacc ctgtggccac cagcttcctc
     1621 cagaaactct cagacctggt gggcagggac acccgactca gcaacctgct gagggagcag
     1681 tggccagaga ggcggcgatg ccacaggttg gagaatggct gtgggaatgc atctcctcag
     1741 atgaaggccc ggaacttgcc tgtttggctc cccaaccagg acgagcccct tcctggcagc
     1801 agctgtgcca tccaagttgg ggataaagtc ccctatgaca tctgccggcc agaccactca
     1861 gtgttgaccc tgcagctgcc tgtgacagcc tccgtgagag aggtgatggc agcgttggcc
     1921 caggaggatg gctggaccaa ggggcaggtg ctggtgaagg tcaattctgc aggtgatgcc
     1981 attggcctgc agccagatgc ccgtggtgtg gccacatctc tggggctcaa tgagcgtctc
     2041 tttgttgtca acccacagga agcgcatgag ctgatcccac accctgacca gctggggccc
     2101 actgtgggct ctgctgaggg gctggacctg gtgagtgcca aggacctggc aggccagctg
     2161 acggaccacg actggagcct cttcaacagt atccaccagg tggagctgat ccactatgtg
     2221 ctgggccccc agcatctgcg ggatgtcacc accgccaacc tggagcgctt catgcgccgc
     2281 ttcaatgagc tgcagtactg ggtggccacc gagctgtgtc tctgccccgt gcccggcccc
     2341 cgggcccagc tgctcaggaa gttcattaag ctggcggccc acctcaagga gcagaagaat
     2401 ctcaattcct tctttgccgt catgtttggc ctcagcaact cggccatcag ccgcctagcc
     2461 cacacctggg agcggctgcc ccacaaagtc cggaagctgt actccgccct cgagaggctg
     2521 ctggatccct catggaacca ccgggtatac cgactggccc tcgccaagct ctcccctcct
     2581 gtcatcccct tcatgcccct tcttgccgca gacatggccg ccattcatga gggaaaccac
     2641 acactagtgg agaatctcat caactttgag aagatgagaa tgatggccag agccgcgcgg
     2701 atgctgcacc actgccgaag ccacaaccct gtgcctctct caccactcag aagccgagtt
     2761 tcccacctcc acgaggacag ccaggtggcg aggatttcca catgctcgga gcagtccctg
     2821 agcacccgga gtccagccag cacctgggct tatgtccagc agctgaaggt cattgacaac
     2881 cagcgggaac tctcccgcct ctcccgagag ctggagccag ctagcgagct catggacggc
     2941 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3001 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     3061 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3121 gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg
     3181 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3241 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     3301 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     3361 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3421 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3481 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3541 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actggaacag ccacaacgtc
     3601 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3661 atcgagccga attttgtctt cctgatcggc gccgcaggaa tactcttcgt atctagcgag
     3721 ctcatggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3781 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     3841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3901 ctcggcatgg acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag
     3961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     4081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     4141 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactggaac
     4381 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     4441 atccgccaca acatcgagcc gaattcctaa ctagagggcc ctattctata gtgtcaccta
     4501 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4561 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4621 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4681 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4741 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4801 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4861 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4921 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4981 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5041 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5101 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5161 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5221 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5581 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5641 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5701 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5761 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5821 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5881 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5941 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     6001 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     6061 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     6121 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     6181 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6241 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6301 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6361 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6421 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     6481 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6541 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6601 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6661 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6721 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     8941 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9001 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     9061 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     9121 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     9181 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     9241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     9301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     9361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     9421 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     9481 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     9541 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     9601 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     9661 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     9721 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     9781 tggcttatcg aaattaatac gactcactat agggagaccc
//
