LOCUS       sequence-451846-        7871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Sec24D protein labeled with mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Westrate LM, Hoyer MJ, Nash MJ, Voeltz GK
  TITLE     Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER 
            to Golgi transport.
  JOURNAL   J Cell Sci. 2020 Jul 24;133(14). pii: jcs.239814. doi: 
            10.1242/jcs.239814.
  PUBMED    32616562
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-07-24"; volume: "133(14). pii: jcs.239814. doi"; 
            pages: " 10.1242/jcs.239814"
COMMENT     Sequence Label: mNeonGreen-Sec24D
FEATURES             Location/Qualifiers
     source          1..7871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1758..2465)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      2201..2210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2462..2471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2510..2713)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2539..2559)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2714..3017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3192..3780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3272..3291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4109..4156
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4181..4200
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4388..5182)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4388..5182)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4499..4518)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5109..5128
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5217..5574)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5231..5366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5383..5574
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5531..5551
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5576..5680)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5707..6162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5794..5813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6004..6025
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6169..6290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6180..6199)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6234..6253
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagtctgctg cctttagtgc ttccatgcca gcctggatga cagaagcaaa gacagtctca
       61 ttttcattag agtctgcaaa catgtctgga atctggtcca acaaattatg aatcacagat
      121 tgggattctt gatagttgac aaggaaacca tccaacaaag gaacaaagac ttctccaaca
      181 tcagtcacca ccatcatctg aggctgggcc agattactct tcacattaaa gaaatggaga
      241 actttgttat atgtgataaa acccactcga attgcagacg tctcttcttg ctcttccttt
      301 ggaatttttt ccagcatggt cttcagttct tcacatatga gcttgacaag tccattcttt
      361 atgttactat atgaaacatc aatcatgaag ataaaggctg gtgggttggg aggcttactc
      421 tttctgcaat aatccaaagt ggcaacatat tcataagatc ctagagataa ctctggtttc
      481 tcatagtggt ccagtcttct tccaatgtgg tccagatgtt ggaaatagaa tggtggaaca
      541 tcattcacac agttgcaaaa tccacactga tatctccttc ctccttcgat gaactgcata
      601 aatgggcaca tgtaggcctt gcacctgttg catctgactg gtccactctc gccgtgattt
      661 accaagtaaa ggggactctc atttgaagga atggtggcaa agggcttgat gacagcagct
      721 aatggaatct gagcttgctt agccatatct gacgtgcatg gaaaacagta tgttgtacaa
      781 cggatgaatc gaggactggc atttccttgg tcttgtatca tgcaatctgt agtgaccagg
      841 ggagggatct ggcctctggt gttggtggca taaacttgtc ctcctctgct ggctctatca
      901 ttctcaatca cctggattgg gctagggata gagtcaggat ccagcttctt ctggggctgt
      961 ggcggaccag ccatctgtgc aggacctcca gggaagcctc ctgggtaaga cagttgtgcg
     1021 cctgccatct ggggaccaga gttggcctgc tgcggaggat atccagcacc caaggtctgg
     1081 cctggaagag gtgggggctg gtactgggca tttggaggag gaggcccaga gagcccatct
     1141 ggtctgtaca ttggtagagg caaaggtgag gcaccaggac cattgagtgt ggtgggtggt
     1201 ggtggaagaa cttgagatcc aggctgcaaa atggaaggct gtggaggtcg tggaggagtc
     1261 tgcaatgatg tggctgacag agggccaggg ggtccctggc ttggaggagc catgcctgaa
     1321 ccatagctgt tgatttgcat agcactgagc tggctgccca gctgggtgac agatgaagtg
     1381 gatataggac ctggataaga agattgtgca gagggttggt atggtgcatg tgaggatgcc
     1441 acattgttga caggtggagg gcctggaaat ctttggggag ggtgaccagt ggcatgagct
     1501 ccattctgac caaactgatg gggtccagga ggtgggggac ccggaggcaa cattccccta
     1561 gtggcggtgg cccccaaagg ccctgctggc ttcatcatac ctgttggaga tgctgtgtgc
     1621 gacggatccc catagtgccc ataatgaggt ggagaaaggc ctattccagg ctgaggctga
     1681 gaatacggag gtgtagccac gtaaccttgt tgactcatgg tggaattcga agcttgagct
     1741 cgagatctga gtccggactt gtacagctcg tccatgccca tcacatcggt aaaggccttt
     1801 tgccactcct tgaagttgag ctcggtcttg gagtgcttga gctccgtctt acggaacacg
     1861 tacatcggct ggttcttcag atagttagcc gccattggct tggcaaaggt gtaggtggtc
     1921 cgcgcagtgc tccggtagcg cttgccattt ccagtggtgt aactccactt aaaggtactg
     1981 atgatggttt tgtcgttggg gtaagtcttc ttcgacctgc accagtccgc agcggtcagc
     2041 gagttggtca tcacaggacc gtcagcaggg aaaccagtcc ccttcacctg ggcctctcct
     2101 ttgatgtggc ttccctcgta ggtgtagcgg tagttaacag taagggaggc accatcttca
     2161 aactgcattg tgcgatggac ttggtagccg gagccatcta ccatggcggc ctggaaaggc
     2221 gacatcccgt cagggtaggg caggtactga tggaagccat acccgatatg agggaccaga
     2281 atccaggggg agaactggag gtcacccttg gtggacttca ggtttaactc ctcataacca
     2341 tcatttggat tgccggtgcc ctgacccacc atgtcaaagt ccacaccgtt gatggagcca
     2401 aagatgtgta actcatgtgt cgctgggaga gaggccatgt tatcctcctc gcccttgctc
     2461 accatggtgg cgaccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta
     2521 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     2581 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     2641 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     2701 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     2761 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     2821 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     2881 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     2941 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     3001 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     3061 attactatta ataactaatg catggcggta atacggttat ccacagaatc aggggataac
     3121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3901 tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc
     3961 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa
     4021 aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc
     4081 cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc
     4141 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca
     4201 gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     4261 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     4321 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
     4381 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     4441 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     4501 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     4561 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     4621 gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg
     4681 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     4741 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     4801 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     4861 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     4921 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     4981 atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     5041 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     5101 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     5161 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg
     5221 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     5281 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     5341 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     5401 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     5461 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     5521 gactttccac accctaactg acacacattc cacagctggt tctttccgcc tcaggactct
     5581 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5701 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5761 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5821 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5881 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5941 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6001 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6061 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6121 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg
     6181 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6241 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     6361 aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatccc
     6421 ctagaaaatt aggcaccaag aaggagatta tctccttgga aatgcaacat caatgacaga
     6481 gaagtttcaa ttaattaagc agctgacaga tctccttgtg aacacaacaa aggaaatcca
     6541 cataagaaga gcctccgtaa agtcctttgt cttctaccag gaactgtcgg aaaaccattt
     6601 ctggttgttc tcgctgcttt acaattgtga gcttcattga atatggcctc ttttgttgga
     6661 taatacccat tatcattctg agttgttgag agtatgggtt tcccacttca ggcagcaatg
     6721 tcatatctgt gttgatatgt gcaaaagatg gcacattaaa tattccttgg atcagttctg
     6781 gtgggctgct tactcccaac cacaggaaca tgtgtagacc attagccagt aagaatattc
     6841 cttcttctga aagacgggac tcagagcaac gaacggcagc aggtaacatt gtactcttga
     6901 catctaacgt gtgtatgggc agaagttgtg ggtagaagaa aagctgagag tcagccacac
     6961 ccatggtcat gaccagctgt ctctggtatg ctcgttcatc agttgagatc tctggtctgc
     7021 tgagtagtac acagtttttc aacaagcaat tcatgtacac tggcaatact ttcatggaat
     7081 ctggtagaat aagctggctt gctgcagaag gacttgcaca attcttccgg taacatgcca
     7141 acatatgggc agtctgatta actagaattt cccggatgac cttcaaaggc tggtggagaa
     7201 ctgctttaaa agctgacttg gcaaagaagt tgataagagc atctgtctca cagctcttat
     7261 aaagatcagc tagctgagag ctgcagttta agccaagatt gtgaatccga agtcttcttt
     7321 gaccactgat tgtcgtgtaa agcacagcac actggattaa ggctccactg tcttcactga
     7381 gtttgtcatc gtgcttgaac tccacggtca ctgccttgtc acaatcgatg gcagccattt
     7441 ctacatcggt ggtgttgttc atcaagattc caccaaagaa atcagtggct ctgaaacctg
     7501 tgctggtacg aaccctcata atagcatcaa agcctatttt cttttcaata tcatttctga
     7561 ggtcgttcaa aaattgttgt ctatccaagt gcatctggaa attgttgtat ttgtaaaggg
     7621 ttcctccagt gagctgagga accagcccca gcgaggccac gtccacatac tgactaggaa
     7681 agaggaagag tgtcacagag cagccgtgag ccacgcagtc cttggccaat gagtcataga
     7741 catttgtttg gggctggaaa agtatcttct ctttgtctgt attaaccagt tttttgtcat
     7801 ctctgttttt gagcttccct ggtgcttcag cagttggcaa ggaagaatgg aagatgaaca
     7861 gcttcccagg a
//
