LOCUS       sequence-451851-       19582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  28(o)C full-length DMD luc-puro-red plasmid. Co-expresses human DMD,
            firefly luciferase, puroR and mCherry in mammalian cells. Plasmid 
            carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 
            37(o)C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   Farruggio AP, Bhakta MS, du Bois H, Ma J, P Calos M
  TITLE     Genomic integration of the full-length dystrophin coding sequence in
            Duchenne muscular dystrophy induced pluripotent stem cells.
  JOURNAL   Biotechnol J. 2017 Jan 31. doi: 10.1002/biot.201600477.
  PUBMED    28139886
REFERENCE   2  (bases 1 to 19582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2017 Jan 31. doi: 10.1002/biot.201600477."
COMMENT     Sequence Label: p28-2iDMD-puro
FEATURES             Location/Qualifiers
     source          1..19582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    43..112
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        402..705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        707..985
                     /label=chicken beta-actin promoter
     intron          986..2000
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1924..1946
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2008..2027
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5246..5257
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             13333..13389
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13390..15039
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(13456..13474)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             15049..15105
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             15712..15768
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             15769..16479
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(15914..15932)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     16182..16201
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    16486..17074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(16539..16559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16957..16968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17089..17106)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17095..17319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    17461..17510
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             17829..18623
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             17829..18623
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(17883..17902)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     18493..18512
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      18948..19536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     19437..19456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
       61 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
      121 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
      181 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
      241 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
      301 gtcgacggta tcgatccata gagcccaccg catccccagc atgcttcata atagtaatca
      361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
      721 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
      781 attttttaat tattttgtgc agcgatgggg gcgggggggg gggggggggc gcgcgccagg
      841 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
      901 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
      961 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     1021 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1081 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1141 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1201 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     1261 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     1321 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     1381 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg ggcgcgtcgg
     1441 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     1501 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     1561 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     1621 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     1681 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     1741 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     1801 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     1861 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     1921 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     1981 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2041 ttggcaaaga attccgccac catgctttgg tgggaagaag tagaggactg ttatgaaaga
     2101 gaagatgttc aaaagaaaac attcacaaaa tgggtaaatg cacaattttc taagtttggg
     2161 aagcagcata ttgagaacct cttcagtgac ctacaggatg ggaggcgcct cctagacctc
     2221 ctcgaaggcc tgacagggca aaaactggta tgtatcaagc ttacaagaca gctttaagga
     2281 gaccaataga aactggggca tgtggagaca gagaagactc ttgggcctcg aggcttcatt
     2341 ttttcttttt ctttttcagc caaaagaaaa aggatccaca agagttcatg ccctgaacaa
     2401 tgtcaacaag gcactgcggg ttttgcagaa caataatgtt gatttagtga atattggaag
     2461 tactgacatc gtagatggaa atcataaact gactcttggt ttgatttgga atataatcct
     2521 ccactggcag gtcaaaaatg taatgaaaaa tatcatggct ggattgcaac aaaccaacag
     2581 tgaaaagatt ctcctgagct gggtccgaca atcaactcgt aattatccac aggttaatgt
     2641 aatcaacttc accaccagct ggtctgatgg cctggctttg aatgctctca tccatagtca
     2701 taggccagac ctatttgact ggaatagtgt ggtttgccag cagtcagcca cacaacgact
     2761 ggaacatgca ttcaacatcg ccagatatca attaggcata gagaaactac tcgatcctga
     2821 agatgttgat accacctatc cagataagaa gtccatctta atgtacatca catcactctt
     2881 ccaagttttg cctcaacaag tgagcatcga agccatccag gaagtggaaa tgttgccaag
     2941 gccacctaaa gtgactaaag aagaacattt tcagttacat catcaaatgc actattctca
     3001 acagatcacg gtcagtctag cacagggata tgagagaact tcttccccta agcctcgatt
     3061 caagagctat gcctacacac aggctgctta tgtcaccacc tctgacccta cacggagccc
     3121 atttccttca cagcatttgg aagctcctga agacaagtca tttggcagtt cattgatgga
     3181 gagtgaagta aacctggacc gttatcaaac agctttagaa gaagtattat cgtggcttct
     3241 ttctgctgag gacacattgc aagcacaagg agagatttct aatgatgtgg aagtggtgaa
     3301 agaccagttt catactcatg aggggtacat gatggatttg acagcccatc agggccgggt
     3361 tggtaatatt ctacaattgg gaagtaagct gattggaaca ggaaaattat cagaagatga
     3421 agaaactgaa gtacaagagc agatgaatct cctaaattca agatgggaat gcctcagggt
     3481 agctagcatg gaaaaacaaa gcaatttaca tagagtttta atggatctcc agaatcagaa
     3541 actgaaagag ttgaatgact ggctaacaaa aacagaagaa agaacaagga aaatggagga
     3601 agagcctctt ggacctgatc ttgaagacct aaaacgccaa gtacaacaac ataaggtgct
     3661 tcaagaagat ctagaacaag aacaagtcag ggtcaattct ctcactcaca tggtggtggt
     3721 agttgatgaa tctagtggag atcacgcaac tgctgctttg gaagaacaac ttaaggtatt
     3781 gggagatcga tgggcaaaca tctgtagatg gacagaagac cgctgggttc ttttacaaga
     3841 catccttctc aaatggcaac gtcttactga agaacagtgc ctttttagtg catggctttc
     3901 agaaaaagaa gatgcagtga acaagattca cacaactggc tttaaagatc aaaatgaaat
     3961 gttatcaagt cttcaaaaac tggccgtttt aaaagcggat ctagaaaaga aaaagcaatc
     4021 catgggcaaa ctgtattcac tcaaacaaga tcttctttca acactgaaga ataagtcagt
     4081 gacccagaag acggaagcat ggctggataa ctttgcccgg tgttgggata atttagtcca
     4141 aaaacttgaa aagagtacag cacagatttc acaggctgtc accaccactc agccatcact
     4201 aacacagaca actgtaatgg aaacagtaac tacggtgacc acaagggaac agatcctggt
     4261 aaagcatgct caagaggaac ttccaccacc acctccccaa aagaagaggc agattactgt
     4321 ggattctgaa attaggaaaa ggttggatgt tgatataact gaacttcaca gctggattac
     4381 tcgctcagaa gctgtgttgc agagtcctga atttgcaatc tttcggaagg aaggcaactt
     4441 ctcagactta aaagaaaaag tcaatgccat agagcgagaa aaagctgaga agttcagaaa
     4501 actgcaagat gccagcagat cagctcaggc cctggtggaa cagatggtga atgagggtgt
     4561 taatgcagat agcatcaaac aagcctcaga acaactgaac agccggtgga tcgaattctg
     4621 ccagttgcta agtgagagac ttaactggct ggagtatcag aacaacatca tcgctttcta
     4681 taatcagcta caacaattgg agcagatgac aactactgct gaaaactggt tgaaaatcca
     4741 acccaccacc ccatcagagc caacagcaat taaaagtcag ttaaaaattt gtaaggatga
     4801 agtcaaccgg ctatcaggtc ttcaacctca aattgaacga ttaaaaattc aaagcatagc
     4861 cctgaaagag aaaggacaag gacccatgtt cctggatgca gactttgtgg cctttacaaa
     4921 tcattttaag caagtctttt ctgatgtgca ggccagagag aaagagctac agacaatttt
     4981 tgacactttg ccaccaatgc gctatcagga gaccatgagt gccatcagga catgggtcca
     5041 gcagtcagaa accaaactct ccatacctca acttagtgtc accgactatg aaatcatgga
     5101 gcagagactc ggggaattgc aggctttaca aagttctctg caagagcaac aaagtggcct
     5161 atactatctc agcaccactg tgaaagagat gtcgaagaaa gcgccctctg aaattagccg
     5221 gaaatatcaa tcagaatttg aagaaattga gggacgctgg aagaagctct cctcccagct
     5281 ggttgagcat tgtcaaaagc tagaggagca aatgaataaa ctccgaaaaa ttcagaatca
     5341 catacaaacc ctgaagaaat ggatggctga agttgatgtt tttctgaagg aggaatggcc
     5401 tgcccttggg gattcagaaa ttctaaaaaa gcagctgaaa cagtgcagac ttttagtcag
     5461 tgatattcag acaattcagc ccagtctaaa cagtgtcaat gaaggtgggc agaagataaa
     5521 gaatgaagca gagccagagt ttgcttcgag acttgagaca gaactcaaag aacttaacac
     5581 tcagtgggat cacatgtgcc aacaggtcta tgccagaaag gaggccttga agggaggttt
     5641 ggagaaaact gtaagcctcc agaaagatct atcagagatg cacgaatgga tgacacaagc
     5701 tgaagaagag tatcttgaga gagattttga atataaaact ccagatgaat tacagaaagc
     5761 agttgaagag atgaagagag ctaaagaaga ggcccaacaa aaagaagcga aagtgaaact
     5821 ccttactgag tctgtaaata gtgtcatagc tcaagctcca cctgtagcac aagaggcctt
     5881 aaaaaaggaa cttgaaactc taaccaccaa ctaccagtgg ctctgcacta ggctgaatgg
     5941 gaaatgcaag actttggaag aagtttgggc atgttggcat gagttattgt catacttgga
     6001 gaaagcaaac aagtggctaa atgaagtaga atttaaactt aaaaccactg aaaacattcc
     6061 tggcggagct gaggaaatct ctgaggtgct agattcactt gaaaatttga tgcgacattc
     6121 agaggataac ccaaatcaga ttcgcatatt ggcacagacc ctaacagatg gcggagtcat
     6181 ggatgagcta atcaatgagg aacttgagac atttaattct cgttggaggg aactacatga
     6241 agaggctgta aggaggcaaa agttgcttga acagagcatc cagtctgccc aggagactga
     6301 aaaatcctta cacttaatcc aggagtccct cacattcatt gacaagcagt tggcagctta
     6361 tattgcagac aaggtggacg cagctcaaat gcctcaggaa gcccagaaaa tccaatctga
     6421 tttgacaagt catgagatca gtttagaaga aatgaagaaa cataatcagg ggaaggaggc
     6481 tgcccaaaga gtcctgtctc agattgatgt tgcacagaaa aaattacaag atgtctccat
     6541 gaagtttcga ttattccaga aaccagccaa ttttgagcag cgtctacaag aaagtaagat
     6601 gattttagat gaagtgaaga tgcacttgcc tgcattggaa acaaagagtg tggaacagga
     6661 agtagtacag tcacagctaa atcattgtgt gaacttgtat aaaagtctga gtgaagtgaa
     6721 gtctgaagtg gaaatggtga taaagactgg acgtcagatt gtacagaaaa agcagacgga
     6781 aaatcccaaa gaacttgatg aaagagtaac agctttgaaa ttgcattata atgagctggg
     6841 agcaaaggta acagaaagaa agcaacagtt ggagaaatgc ttgaaattgt cccgtaagat
     6901 gcgaaaggaa atgaatgtct tgacagaatg gctggcagct acagatatgg aattgacaaa
     6961 gagatcagca gttgaaggaa tgcctagtaa tttggattct gaagttgcct ggggaaaggc
     7021 tactcaaaaa gagattgaga aacagaaggt gcacctgaag agtatcacag aggtaggaga
     7081 ggccttgaaa acagttttgg gcaagaagga gacgttggtg gaagataaac tcagtcttct
     7141 gaatagtaat tggatagctg tcacctcccg agcagaagag tggttaaatc ttttgttgga
     7201 ataccagaaa cacatggaaa cttttgacca gaatgtggac cacatcacaa agtggatcat
     7261 tcaggctgac acacttttgg atgaatcaga gaaaaagaaa ccccagcaaa aagaagacgt
     7321 gcttaagcgt ttaaaggcag aactgaatga catacgccca aaggtggact ctacacgtga
     7381 ccaagcagca aacttgatgg caaaccacgg tgaccactgc aggaaattag tagagcccca
     7441 aatctcagag ctcaaccatc gatttgcagc catttcacac agaattaaga ctggaaaggc
     7501 ctccattcct ttgaaggaat tggagcagtt taactcagat atacaaaaat tgcttgaacc
     7561 actggaggct gaaattcagc agggggtgaa tctgaaagag gaagacttca ataaagatat
     7621 gaatgaagac aatgagggta ctgtaaaaga attgttgcaa agaggagaca acttacaaca
     7681 aagaatcaca gatgagagaa agcgagagga aataaagata aaacagcagc tgttacagac
     7741 aaaacataat gctctcaagg atttgaggtc tcaaagaaga aaaaaggctc tagaaatttc
     7801 tcatcagtgg tatcagtaca agaggcaggc tgatgatctc ctgaaatgct tggatgacat
     7861 tgaaaaaaaa ttagccagcc tacctgagcc cagagatgaa aggaaaataa aggaaattga
     7921 tcgggaattg cagaagaaga aagaggagct gaatgcagtg cgtaggcaag ctgagggctt
     7981 gtctgaggat ggggccgcaa tggcagtgga gccaactcag atccagctca gcaagcgctg
     8041 gcgggaaatt gagagcaaat ttgctcagtt tcgaagactc aactttgcac aaattcacac
     8101 tgtccgtgaa gaaacgatga tggtgatgac tgaagacatg cctttggaaa tttcttatgt
     8161 gccttctact tatttgactg aaatcactca tgtctcacaa gccctattag aagtggaaca
     8221 acttctcaat gctcctgacc tctgtgctaa ggactttgaa gatctcttta agcaagagga
     8281 gtctctgaag aatataaaag atagtctaca acaaagctca ggtcggattg acattattca
     8341 tagcaagaag acagcagcat tgcaaagtgc aacgcctgtg gaaagggtga agctacagga
     8401 agctctctcc cagcttgatt tccaatggga aaaagttaac aaaatgtaca aggaccgaca
     8461 agggcgattt gacagatctg ttgagaaatg gcggcgtttt cattatgata taaagatatt
     8521 taatcagtgg ctaacagaag ctgaacagtt tctcagaaag acacaaattc ctgagaattg
     8581 ggaacatgct aaatacaaat ggtatcttaa ggaactccag gatggcattg ggcagcggca
     8641 aactgttgtc agaacattga atgcaactgg ggaagaaata attcagcaat cctcaaaaac
     8701 agatgccagt attctacagg aaaaattggg aagcctgaat ctgcggtggc aggaggtctg
     8761 caaacagctg tcagacagaa aaaagaggct agaagaacaa aagaatatct tgtcagaatt
     8821 tcaaagagat ttaaatgaat ttgttttatg gttggaggaa gcagataaca ttgctagtat
     8881 cccacttgaa cctggaaaag agcagcaact aaaagaaaag cttgagcaag tcaagttact
     8941 ggtggaagag ttgcccctgc gccagggccg aattctcaaa caattaaatg aaactggagg
     9001 acccgtgctt gtaagtgctc ccataagccc agaagagcaa gataaacttg aaaataagct
     9061 caagcagaca aatctccagt ggataaaggt ttccagagct ttacctgaga aacaaggaga
     9121 aattgaagct caaataaaag accttgggca gcttgaaaaa aagcttgaag accttgaaga
     9181 gcagttaaat catctgctgc tgtggttatc tcctattagg aatcagttgg aaatttataa
     9241 ccaaccaaac caagaaggac catttgacgt taaggaaact gaaatagcag ttcaagctaa
     9301 acaaccggat gtggaagaga ttttgtctaa agggcagcat ttgtacaagg aaaaaccagc
     9361 cactcagcca gtgaagagga agttagaaga tctgagctct gagtggaagg cggtaaaccg
     9421 tttacttcaa gagctgaggg caaagcagcc tgacctagct cctggactga ccactattgg
     9481 agcctctcct actcagactg ttactctggt gacacaacct gtggttacta aggaaactgc
     9541 catctccaaa ctagaaatgc catcttcctt gatgttggag gtacctgctc tggcagattt
     9601 caaccgggct tggacagaac ttaccgactg gctttctctg cttgatcaag ttataaaatc
     9661 acagagggtg atggtgggtg accttgagga tatcaacgag atgatcatca agcagaaggc
     9721 gacaatgcag gatttggaac agaggcgtcc ccagttggaa gaactcatta ccgctgccca
     9781 aaatttgaaa aacaagacca gcaatcaaga ggctagaaca atcattacgg atcgaattga
     9841 aagaattcag aatcagtggg atgaagtaca agaacacctt cagaaccgga ggcaacagtt
     9901 gaatgaaatg ttaaaggatt caacacaatg gctggaagct aaggaagaag ctgagcaggt
     9961 cttaggacag gccagagcca agcttgagtc atggaaggag ggtccctata cagtagatgc
    10021 aatccaaaag aaaatcacag aaaccaagca gttggccaaa gacctccgcc agtggcagac
    10081 aaatgtagat gtggcaaatg acttggccct gaaacttctc cgggattatt ctgcagatga
    10141 taccagaaaa gtccacatga taacagagaa tatcaatgcc tcttggagaa gcattcataa
    10201 aagggtgagt gagcgagagg ctgctttgga agaaactcat agattactgc aacagttccc
    10261 cctggacctg gaaaagtttc ttgcctggct tacagaagct gaaacaactg ccaatgtcct
    10321 acaggatgct acccgtaagg aaaggctcct agaagactcc aagggagtaa aagagctgat
    10381 gaaacaatgg caagacctcc aaggtgaaat tgaagctcac acagatgttt atcacaacct
    10441 gggtgaaaac agccaaaaaa tcctgagatc cctggaaggt tccgatgatg cagtcctgtt
    10501 acaaagacgt ttggataaca tgaacttcaa gtggagtgaa cttcggaaaa agtctctcaa
    10561 cattaggtcc catttggaag ccagttctga ccagtggaag cgtctgcacc tttctctgca
    10621 ggaacttctg gtgtggctac agctgaaaga tgatgaatta agccggcagg cacctattgg
    10681 aggcgacttt ccagcagttc agaagcagaa cgatgtacat agggccttca agagggaatt
    10741 gaaaactaaa gaacctgtaa tcatgagtat tcttgagact gtacgaatat ttctgacaga
    10801 gcagcctttg gaaggactag agaaacccta ccaggagccc agagagctgc ctcctgagga
    10861 gagagcccag aatgtcactc ggcttctacg aaagcaggct gaggaggtca atactgagtg
    10921 ggaaaaattg aacctgcact ccgctgactg gcagagaaaa atagatgaga cccttgaaag
    10981 actccaggaa cttcaagagg ccacggatga gctggacctc aagctgcgcc aagctgaggt
    11041 gatcaaggga tcctggcagc ccgtgggcga tctcctcatt gactctctcc aagatcacct
    11101 cgagaaagtc aaggcacttc gaggagaaat tgcgcctctg aaagagaacg tgagccacgt
    11161 caatgacctt gctcgccagc ttaccacttt gggcattcag ctctcaccgt ataacctcag
    11221 cactctggaa gacctgaaca ccagatggaa gcttctgcag gtggccgtcg aggaccgagt
    11281 caggcagctg catgaagccc acagggactt tggtccagca tctcagcact ttctttccac
    11341 gtctgtccag ggtccctggg agagagccat ctcgccaaac aaagtgccct actatatcaa
    11401 ccacgagact caaacaactt gctgggacca tcccaaaatg acagagctct accagtcttt
    11461 agctgacctg aataatgtca gattctcagc ttataggact gccatgaaac tccgaagact
    11521 gcagaaggcc ctttgcttgg atctcttgag cctgtcagct gcatgtgatg ccttggacca
    11581 gcacaacctc aagcaaaatg accagcccat ggatatcctg cagattatta attgtttgac
    11641 cactatttat gaccgcctgg agcaagagca caacaatttg gtcaacgtcc ctctctgcgt
    11701 ggatatgtgt ctgaactggc tgctgaatgt ttatgatacg ggacgaacag ggaggatccg
    11761 tgtcctgtct tttaaaactg gcatcatttc cctgtgtaaa gcacatttgg aagacaagta
    11821 cagatacctt ttcaagcaag tggcaagttc aacaggattt tgtgaccagc gcaggctggg
    11881 cctccttctg catgattcta tccaaattcc aagacagttg ggtgaagttg catcctttgg
    11941 gggcagtaac attgagccaa gtgtccggag ctgcttccaa tttgctaata ataagccaga
    12001 gatcgaagcg gccctcttcc tagactggat gagactggaa ccccagtcca tggtgtggct
    12061 gcccgtcctg cacagagtgg ctgctgcaga aactgccaag catcaggcca aatgtaacat
    12121 ctgcaaagag tgtccaatca ttggattcag gtacaggagt ctaaagcact ttaattatga
    12181 catctgccaa agctgctttt tttctggtcg agttgcaaaa ggccataaaa tgcactatcc
    12241 catggtggaa tattgcactc cgactacatc aggagaagat gttcgagact ttgccaaggt
    12301 actaaaaaac aaatttcgaa ccaaaaggta ttttgcgaag catccccgaa tgggctacct
    12361 gccagtgcag actgtcttag agggggacaa catggaaact cccgttactc tgatcaactt
    12421 ctggccagta gattctgcgc ctgcctcgtc ccctcagctt tcacacgatg atactcattc
    12481 acgcattgaa cattatgcta gcaggctagc agaaatggaa aacagcaatg gatcttatct
    12541 aaatgatagc atctctccta atgagagcat agatgatgaa catttgttaa tccagcatta
    12601 ctgccaaagt ttgaaccagg actcccccct gagccagcct cgtagtcctg cccagatctt
    12661 gatttcctta gagagtgagg aaagagggga gctagagaga atcctagcag atcttgagga
    12721 agaaaacagg aatctgcaag cagaatatga ccgtctaaag cagcagcacg aacataaagg
    12781 cctgtcccca ctgccgtccc ctcctgaaat gatgcccacc tctccccaga gtccccggga
    12841 tgctgagctc attgctgagg ccaagctact gcgtcaacac aaaggccgcc tggaagccag
    12901 gatgcaaatc ctggaagacc acaataaaca gctggagtca cagttacaca ggctaaggca
    12961 gctgctggag caaccccagg cagaggccaa agtgaatggc acaacggtgt cctctccttc
    13021 tacctctcta cagaggtccg acagcagtca gcctatgctg ctccgagtgg ttggcagtca
    13081 aacttcggac tccatgggta agtagagggc tgggctgggc tgtggggggt gtggggtgcg
    13141 ggactgggca gtctgggagt ccctctcacc acttttctta cctttctagg tgaggaagat
    13201 cttctcagtc ctccccagga cacaagcaca gggttagagg aggtgatgga gcaactcaac
    13261 aactccttcc ctagttcaag aggaagaaat acccctggaa agccaatgag agaggacaca
    13321 atgggatccg gtgccacgaa cttctctctg ttaaagcaag caggagatgt ggaagaaaac
    13381 cccggtccta tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
    13441 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
    13501 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
    13561 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
    13621 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
    13681 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
    13741 atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg
    13801 aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat
    13861 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
    13921 gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac
    13981 tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg
    14041 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
    14101 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
    14161 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt
    14221 caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa
    14281 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct
    14341 cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg
    14401 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
    14461 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
    14521 accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt
    14581 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
    14641 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc
    14701 ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc
    14761 ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc
    14821 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
    14881 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
    14941 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
    15001 atcctcataa aggccaagaa gggcggaaag atcgccgtgg gatccggtgc cacgaacttc
    15061 tctctgttaa agcaagcagg agatgtggaa gaaaaccccg gtcctatgac tgaatacaaa
    15121 ccaactgttc gcctggcaac tcgtgatgat gttccacgtg cagttcgcac cctggctgct
    15181 gcatttgctg actaccctgc aacccgtcac actgtggacc cagaccgcca cattgaacgt
    15241 gtgactgaac tgcaggagct gttcctgacc cgtgtgggcc tggacattgg caaagtgtgg
    15301 gtggcagatg atggtgctgc tgtggcagtg tggaccaccc ctgaatctgt tgaagctggt
    15361 gcagtgtttg ctgagattgg cccacgcatg gcagaactgt ctggcagccg cctggcagca
    15421 caacagcaga tggaaggtct gctggcacca caccgcccaa aagaacctgc ttggttcctg
    15481 gcaactgtgg gtgtgagccc tgaccaccag ggtaagggcc tgggctctgc agtggtgctg
    15541 cctggtgtgg aagcagctga acgtgcaggt gtgcctgctt tcctggagac ctcagctcca
    15601 cgcaacctgc ctttctatga acgcctgggc ttcactgtga ctgctgatgt ggaagtgcca
    15661 gaaggcccac gcacttggtg catgactcgc aaaccaggtg ctggatccgg tgccacgaac
    15721 ttctctctgt taaagcaagc aggagatgtg gaagaaaacc ccggtcctat ggtgagcaag
    15781 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
    15841 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
    15901 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
    15961 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
    16021 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
    16081 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
    16141 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
    16201 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
    16261 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
    16321 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
    16381 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
    16441 cgccactcca ccggcggcat ggacgagctg tacaagtaaa tcgataatca acctctggat
    16501 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    16561 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    16621 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    16681 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    16741 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    16801 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    16861 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    16921 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    16981 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    17041 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat actcgagccc tcgactgtgc
    17101 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
    17161 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
    17221 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
    17281 acaatagcag gcatgctggg gatgcggtgg gctctatggg gcgcgcgaaa ccggtgaaaa
    17341 accgctcagc ggcgcccgca cccaaacgaa aacacccccg gcccaagcgg accgggggtg
    17401 tcgcgtgaaa tggatcagtg agcgtggccg tggtggtggc cgtggccgtg ctcgtcctcg
    17461 tcggccggct tgtcgacgac ggcggtctcc gtcgtcagga tcatccgggc caccgaggcg
    17521 gcgttgagaa cagccgagcg ggtcaccttg gccggatcga cgacgccggc ggagaccaga
    17581 tcaccgaact ccagcgtggc ggcgttgaag ccctgcccct tgggaagttc ggagattaat
    17641 taaccgttta aattgggcgc gccaaggaag ctagcaggac agcaagcgaa ccggaattgc
    17701 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
    17761 tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
    17821 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
    17881 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
    17941 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
    18001 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
    18061 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
    18121 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
    18181 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
    18241 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
    18301 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
    18361 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
    18421 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
    18481 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
    18541 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
    18601 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
    18661 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
    18721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    18781 caataaccct gataaatgct tcaataatag cacgtgctaa aacttcattt ttaatttaaa
    18841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    18901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    18961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    19021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    19081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    19141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    19201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    19261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    19321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    19381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    19441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    19501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    19561 cggttcctgg gcttttgctg gc
//
