LOCUS       sequence-451853-       18887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  37(o)C full-length DMD luc-red plasmid. Co-expresses human 
            dystrophin, firefly luciferase and mCherry in mammalian cells. 
            Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 
            37(o)C..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18887)
  AUTHORS   Farruggio AP, Bhakta MS, du Bois H, Ma J, P Calos M
  TITLE     Genomic integration of the full-length dystrophin coding sequence in
            Duchenne muscular dystrophy induced pluripotent stem cells.
  JOURNAL   Biotechnol J. 2017 Jan 31. doi: 10.1002/biot.201600477.
  PUBMED    28139886
REFERENCE   2  (bases 1 to 18887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2017 Jan 31. doi: 10.1002/biot.201600477."
COMMENT     Sequence Label: p37-2iDMD-LR
FEATURES             Location/Qualifiers
     source          1..18887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    43..112
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        402..705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        707..987
                     /label=chicken beta-actin promoter
     intron          988..2002
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1926..1948
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2010..2029
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5248..5259
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             13335..13391
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13392..15041
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(13458..13476)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             15051..15107
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             15108..15818
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(15253..15271)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     15521..15540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    15825..16413
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(15878..15898)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16296..16307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(16428..16445)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    16434..16658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    16800..16849
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             17168..17962
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             17168..17962
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(17222..17241)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     17832..17851
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      18251..18839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18740..18759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
       61 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
      121 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
      181 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
      241 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
      301 gtcgacggta tcgatccata gagcccaccg catccccagc atgcttcata atagtaatca
      361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
      721 acgttctgct tcactctccc catctccccc cccctcccca cccccaattt tgtatttatt
      781 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggg gcgcgcgcca
      841 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
      901 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      961 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     1021 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1081 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1141 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1201 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     1261 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1321 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     1381 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgggcgcgtc
     1441 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     1501 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     1561 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     1621 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     1681 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     1741 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     1801 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     1861 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     1921 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     1981 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2041 ttttggcaaa gaattccgcc accatgcttt ggtgggaaga agtagaggac tgttatgaaa
     2101 gagaagatgt tcaaaagaaa acattcacaa aatgggtaaa tgcacaattt tctaagtttg
     2161 ggaagcagca tattgagaac ctcttcagtg acctacagga tgggaggcgc ctcctagacc
     2221 tcctcgaagg cctgacaggg caaaaactgg tatgtatcaa gcttacaaga cagctttaag
     2281 gagaccaata gaaactgggg catgtggaga cagagaagac tcttgggcct cgaggcttca
     2341 ttttttcttt ttctttttca gccaaaagaa aaaggatcca caagagttca tgccctgaac
     2401 aatgtcaaca aggcactgcg ggttttgcag aacaataatg ttgatttagt gaatattgga
     2461 agtactgaca tcgtagatgg aaatcataaa ctgactcttg gtttgatttg gaatataatc
     2521 ctccactggc aggtcaaaaa tgtaatgaaa aatatcatgg ctggattgca acaaaccaac
     2581 agtgaaaaga ttctcctgag ctgggtccga caatcaactc gtaattatcc acaggttaat
     2641 gtaatcaact tcaccaccag ctggtctgat ggcctggctt tgaatgctct catccatagt
     2701 cataggccag acctatttga ctggaatagt gtggtttgcc agcagtcagc cacacaacga
     2761 ctggaacatg cattcaacat cgccagatat caattaggca tagagaaact actcgatcct
     2821 gaagatgttg ataccaccta tccagataag aagtccatct taatgtacat cacatcactc
     2881 ttccaagttt tgcctcaaca agtgagcatc gaagccatcc aggaagtgga aatgttgcca
     2941 aggccaccta aagtgactaa agaagaacat tttcagttac atcatcaaat gcactattct
     3001 caacagatca cggtcagtct agcacaggga tatgagagaa cttcttcccc taagcctcga
     3061 ttcaagagct atgcctacac acaggctgct tatgtcacca cctctgaccc tacacggagc
     3121 ccatttcctt cacagcattt ggaagctcct gaagacaagt catttggcag ttcattgatg
     3181 gagagtgaag taaacctgga ccgttatcaa acagctttag aagaagtatt atcgtggctt
     3241 ctttctgctg aggacacatt gcaagcacaa ggagagattt ctaatgatgt ggaagtggtg
     3301 aaagaccagt ttcatactca tgaggggtac atgatggatt tgacagccca tcagggccgg
     3361 gttggtaata ttctacaatt gggaagtaag ctgattggaa caggaaaatt atcagaagat
     3421 gaagaaactg aagtacaaga gcagatgaat ctcctaaatt caagatggga atgcctcagg
     3481 gtagctagca tggaaaaaca aagcaattta catagagttt taatggatct ccagaatcag
     3541 aaactgaaag agttgaatga ctggctaaca aaaacagaag aaagaacaag gaaaatggag
     3601 gaagagcctc ttggacctga tcttgaagac ctaaaacgcc aagtacaaca acataaggtg
     3661 cttcaagaag atctagaaca agaacaagtc agggtcaatt ctctcactca catggtggtg
     3721 gtagttgatg aatctagtgg agatcacgca actgctgctt tggaagaaca acttaaggta
     3781 ttgggagatc gatgggcaaa catctgtaga tggacagaag accgctgggt tcttttacaa
     3841 gacatccttc tcaaatggca acgtcttact gaagaacagt gcctttttag tgcatggctt
     3901 tcagaaaaag aagatgcagt gaacaagatt cacacaactg gctttaaaga tcaaaatgaa
     3961 atgttatcaa gtcttcaaaa actggccgtt ttaaaagcgg atctagaaaa gaaaaagcaa
     4021 tccatgggca aactgtattc actcaaacaa gatcttcttt caacactgaa gaataagtca
     4081 gtgacccaga agacggaagc atggctggat aactttgccc ggtgttggga taatttagtc
     4141 caaaaacttg aaaagagtac agcacagatt tcacaggctg tcaccaccac tcagccatca
     4201 ctaacacaga caactgtaat ggaaacagta actacggtga ccacaaggga acagatcctg
     4261 gtaaagcatg ctcaagagga acttccacca ccacctcccc aaaagaagag gcagattact
     4321 gtggattctg aaattaggaa aaggttggat gttgatataa ctgaacttca cagctggatt
     4381 actcgctcag aagctgtgtt gcagagtcct gaatttgcaa tctttcggaa ggaaggcaac
     4441 ttctcagact taaaagaaaa agtcaatgcc atagagcgag aaaaagctga gaagttcaga
     4501 aaactgcaag atgccagcag atcagctcag gccctggtgg aacagatggt gaatgagggt
     4561 gttaatgcag atagcatcaa acaagcctca gaacaactga acagccggtg gatcgaattc
     4621 tgccagttgc taagtgagag acttaactgg ctggagtatc agaacaacat catcgctttc
     4681 tataatcagc tacaacaatt ggagcagatg acaactactg ctgaaaactg gttgaaaatc
     4741 caacccacca ccccatcaga gccaacagca attaaaagtc agttaaaaat ttgtaaggat
     4801 gaagtcaacc ggctatcagg tcttcaacct caaattgaac gattaaaaat tcaaagcata
     4861 gccctgaaag agaaaggaca aggacccatg ttcctggatg cagactttgt ggcctttaca
     4921 aatcatttta agcaagtctt ttctgatgtg caggccagag agaaagagct acagacaatt
     4981 tttgacactt tgccaccaat gcgctatcag gagaccatga gtgccatcag gacatgggtc
     5041 cagcagtcag aaaccaaact ctccatacct caacttagtg tcaccgacta tgaaatcatg
     5101 gagcagagac tcggggaatt gcaggcttta caaagttctc tgcaagagca acaaagtggc
     5161 ctatactatc tcagcaccac tgtgaaagag atgtcgaaga aagcgccctc tgaaattagc
     5221 cggaaatatc aatcagaatt tgaagaaatt gagggacgct ggaagaagct ctcctcccag
     5281 ctggttgagc attgtcaaaa gctagaggag caaatgaata aactccgaaa aattcagaat
     5341 cacatacaaa ccctgaagaa atggatggct gaagttgatg tttttctgaa ggaggaatgg
     5401 cctgcccttg gggattcaga aattctaaaa aagcagctga aacagtgcag acttttagtc
     5461 agtgatattc agacaattca gcccagtcta aacagtgtca atgaaggtgg gcagaagata
     5521 aagaatgaag cagagccaga gtttgcttcg agacttgaga cagaactcaa agaacttaac
     5581 actcagtggg atcacatgtg ccaacaggtc tatgccagaa aggaggcctt gaagggaggt
     5641 ttggagaaaa ctgtaagcct ccagaaagat ctatcagaga tgcacgaatg gatgacacaa
     5701 gctgaagaag agtatcttga gagagatttt gaatataaaa ctccagatga attacagaaa
     5761 gcagttgaag agatgaagag agctaaagaa gaggcccaac aaaaagaagc gaaagtgaaa
     5821 ctccttactg agtctgtaaa tagtgtcata gctcaagctc cacctgtagc acaagaggcc
     5881 ttaaaaaagg aacttgaaac tctaaccacc aactaccagt ggctctgcac taggctgaat
     5941 gggaaatgca agactttgga agaagtttgg gcatgttggc atgagttatt gtcatacttg
     6001 gagaaagcaa acaagtggct aaatgaagta gaatttaaac ttaaaaccac tgaaaacatt
     6061 cctggcggag ctgaggaaat ctctgaggtg ctagattcac ttgaaaattt gatgcgacat
     6121 tcagaggata acccaaatca gattcgcata ttggcacaga ccctaacaga tggcggagtc
     6181 atggatgagc taatcaatga ggaacttgag acatttaatt ctcgttggag ggaactacat
     6241 gaagaggctg taaggaggca aaagttgctt gaacagagca tccagtctgc ccaggagact
     6301 gaaaaatcct tacacttaat ccaggagtcc ctcacattca ttgacaagca gttggcagct
     6361 tatattgcag acaaggtgga cgcagctcaa atgcctcagg aagcccagaa aatccaatct
     6421 gatttgacaa gtcatgagat cagtttagaa gaaatgaaga aacataatca ggggaaggag
     6481 gctgcccaaa gagtcctgtc tcagattgat gttgcacaga aaaaattaca agatgtctcc
     6541 atgaagtttc gattattcca gaaaccagcc aattttgagc agcgtctaca agaaagtaag
     6601 atgattttag atgaagtgaa gatgcacttg cctgcattgg aaacaaagag tgtggaacag
     6661 gaagtagtac agtcacagct aaatcattgt gtgaacttgt ataaaagtct gagtgaagtg
     6721 aagtctgaag tggaaatggt gataaagact ggacgtcaga ttgtacagaa aaagcagacg
     6781 gaaaatccca aagaacttga tgaaagagta acagctttga aattgcatta taatgagctg
     6841 ggagcaaagg taacagaaag aaagcaacag ttggagaaat gcttgaaatt gtcccgtaag
     6901 atgcgaaagg aaatgaatgt cttgacagaa tggctggcag ctacagatat ggaattgaca
     6961 aagagatcag cagttgaagg aatgcctagt aatttggatt ctgaagttgc ctggggaaag
     7021 gctactcaaa aagagattga gaaacagaag gtgcacctga agagtatcac agaggtagga
     7081 gaggccttga aaacagtttt gggcaagaag gagacgttgg tggaagataa actcagtctt
     7141 ctgaatagta attggatagc tgtcacctcc cgagcagaag agtggttaaa tcttttgttg
     7201 gaataccaga aacacatgga aacttttgac cagaatgtgg accacatcac aaagtggatc
     7261 attcaggctg acacactttt ggatgaatca gagaaaaaga aaccccagca aaaagaagac
     7321 gtgcttaagc gtttaaaggc agaactgaat gacatacgcc caaaggtgga ctctacacgt
     7381 gaccaagcag caaacttgat ggcaaaccac ggtgaccact gcaggaaatt agtagagccc
     7441 caaatctcag agctcaacca tcgatttgca gccatttcac acagaattaa gactggaaag
     7501 gcctccattc ctttgaagga attggagcag tttaactcag atatacaaaa attgcttgaa
     7561 ccactggagg ctgaaattca gcagggggtg aatctgaaag aggaagactt caataaagat
     7621 atgaatgaag acaatgaggg tactgtaaaa gaattgttgc aaagaggaga caacttacaa
     7681 caaagaatca cagatgagag aaagcgagag gaaataaaga taaaacagca gctgttacag
     7741 acaaaacata atgctctcaa ggatttgagg tctcaaagaa gaaaaaaggc tctagaaatt
     7801 tctcatcagt ggtatcagta caagaggcag gctgatgatc tcctgaaatg cttggatgac
     7861 attgaaaaaa aattagccag cctacctgag cccagagatg aaaggaaaat aaaggaaatt
     7921 gatcgggaat tgcagaagaa gaaagaggag ctgaatgcag tgcgtaggca agctgagggc
     7981 ttgtctgagg atggggccgc aatggcagtg gagccaactc agatccagct cagcaagcgc
     8041 tggcgggaaa ttgagagcaa atttgctcag tttcgaagac tcaactttgc acaaattcac
     8101 actgtccgtg aagaaacgat gatggtgatg actgaagaca tgcctttgga aatttcttat
     8161 gtgccttcta cttatttgac tgaaatcact catgtctcac aagccctatt agaagtggaa
     8221 caacttctca atgctcctga cctctgtgct aaggactttg aagatctctt taagcaagag
     8281 gagtctctga agaatataaa agatagtcta caacaaagct caggtcggat tgacattatt
     8341 catagcaaga agacagcagc attgcaaagt gcaacgcctg tggaaagggt gaagctacag
     8401 gaagctctct cccagcttga tttccaatgg gaaaaagtta acaaaatgta caaggaccga
     8461 caagggcgat ttgacagatc tgttgagaaa tggcggcgtt ttcattatga tataaagata
     8521 tttaatcagt ggctaacaga agctgaacag tttctcagaa agacacaaat tcctgagaat
     8581 tgggaacatg ctaaatacaa atggtatctt aaggaactcc aggatggcat tgggcagcgg
     8641 caaactgttg tcagaacatt gaatgcaact ggggaagaaa taattcagca atcctcaaaa
     8701 acagatgcca gtattctaca ggaaaaattg ggaagcctga atctgcggtg gcaggaggtc
     8761 tgcaaacagc tgtcagacag aaaaaagagg ctagaagaac aaaagaatat cttgtcagaa
     8821 tttcaaagag atttaaatga atttgtttta tggttggagg aagcagataa cattgctagt
     8881 atcccacttg aacctggaaa agagcagcaa ctaaaagaaa agcttgagca agtcaagtta
     8941 ctggtggaag agttgcccct gcgccagggc cgaattctca aacaattaaa tgaaactgga
     9001 ggacccgtgc ttgtaagtgc tcccataagc ccagaagagc aagataaact tgaaaataag
     9061 ctcaagcaga caaatctcca gtggataaag gtttccagag ctttacctga gaaacaagga
     9121 gaaattgaag ctcaaataaa agaccttggg cagcttgaaa aaaagcttga agaccttgaa
     9181 gagcagttaa atcatctgct gctgtggtta tctcctatta ggaatcagtt ggaaatttat
     9241 aaccaaccaa accaagaagg accatttgac gttaaggaaa ctgaaatagc agttcaagct
     9301 aaacaaccgg atgtggaaga gattttgtct aaagggcagc atttgtacaa ggaaaaacca
     9361 gccactcagc cagtgaagag gaagttagaa gatctgagct ctgagtggaa ggcggtaaac
     9421 cgtttacttc aagagctgag ggcaaagcag cctgacctag ctcctggact gaccactatt
     9481 ggagcctctc ctactcagac tgttactctg gtgacacaac ctgtggttac taaggaaact
     9541 gccatctcca aactagaaat gccatcttcc ttgatgttgg aggtacctgc tctggcagat
     9601 ttcaaccggg cttggacaga acttaccgac tggctttctc tgcttgatca agttataaaa
     9661 tcacagaggg tgatggtggg tgaccttgag gatatcaacg agatgatcat caagcagaag
     9721 gcgacaatgc aggatttgga acagaggcgt ccccagttgg aagaactcat taccgctgcc
     9781 caaaatttga aaaacaagac cagcaatcaa gaggctagaa caatcattac ggatcgaatt
     9841 gaaagaattc agaatcagtg ggatgaagta caagaacacc ttcagaaccg gaggcaacag
     9901 ttgaatgaaa tgttaaagga ttcaacacaa tggctggaag ctaaggaaga agctgagcag
     9961 gtcttaggac aggccagagc caagcttgag tcatggaagg agggtcccta tacagtagat
    10021 gcaatccaaa agaaaatcac agaaaccaag cagttggcca aagacctccg ccagtggcag
    10081 acaaatgtag atgtggcaaa tgacttggcc ctgaaacttc tccgggatta ttctgcagat
    10141 gataccagaa aagtccacat gataacagag aatatcaatg cctcttggag aagcattcat
    10201 aaaagggtga gtgagcgaga ggctgctttg gaagaaactc atagattact gcaacagttc
    10261 cccctggacc tggaaaagtt tcttgcctgg cttacagaag ctgaaacaac tgccaatgtc
    10321 ctacaggatg ctacccgtaa ggaaaggctc ctagaagact ccaagggagt aaaagagctg
    10381 atgaaacaat ggcaagacct ccaaggtgaa attgaagctc acacagatgt ttatcacaac
    10441 ctgggtgaaa acagccaaaa aatcctgaga tccctggaag gttccgatga tgcagtcctg
    10501 ttacaaagac gtttggataa catgaacttc aagtggagtg aacttcggaa aaagtctctc
    10561 aacattaggt cccatttgga agccagttct gaccagtgga agcgtctgca cctttctctg
    10621 caggaacttc tggtgtggct acagctgaaa gatgatgaat taagccggca ggcacctatt
    10681 ggaggcgact ttccagcagt tcagaagcag aacgatgtac atagggcctt caagagggaa
    10741 ttgaaaacta aagaacctgt aatcatgagt attcttgaga ctgtacgaat atttctgaca
    10801 gagcagcctt tggaaggact agagaaaccc taccaggagc ccagagagct gcctcctgag
    10861 gagagagccc agaatgtcac tcggcttcta cgaaagcagg ctgaggaggt caatactgag
    10921 tgggaaaaat tgaacctgca ctccgctgac tggcagagaa aaatagatga gacccttgaa
    10981 agactccagg aacttcaaga ggccacggat gagctggacc tcaagctgcg ccaagctgag
    11041 gtgatcaagg gatcctggca gcccgtgggc gatctcctca ttgactctct ccaagatcac
    11101 ctcgagaaag tcaaggcact tcgaggagaa attgcgcctc tgaaagagaa cgtgagccac
    11161 gtcaatgacc ttgctcgcca gcttaccact ttgggcattc agctctcacc gtataacctc
    11221 agcactctgg aagacctgaa caccagatgg aagcttctgc aggtggccgt cgaggaccga
    11281 gtcaggcagc tgcatgaagc ccacagggac tttggtccag catctcagca ctttctttcc
    11341 acgtctgtcc agggtccctg ggagagagcc atctcgccaa acaaagtgcc ctactatatc
    11401 aaccacgaga ctcaaacaac ttgctgggac catcccaaaa tgacagagct ctaccagtct
    11461 ttagctgacc tgaataatgt cagattctca gcttatagga ctgccatgaa actccgaaga
    11521 ctgcagaagg ccctttgctt ggatctcttg agcctgtcag ctgcatgtga tgccttggac
    11581 cagcacaacc tcaagcaaaa tgaccagccc atggatatcc tgcagattat taattgtttg
    11641 accactattt atgaccgcct ggagcaagag cacaacaatt tggtcaacgt ccctctctgc
    11701 gtggatatgt gtctgaactg gctgctgaat gtttatgata cgggacgaac agggaggatc
    11761 cgtgtcctgt cttttaaaac tggcatcatt tccctgtgta aagcacattt ggaagacaag
    11821 tacagatacc ttttcaagca agtggcaagt tcaacaggat tttgtgacca gcgcaggctg
    11881 ggcctccttc tgcatgattc tatccaaatt ccaagacagt tgggtgaagt tgcatccttt
    11941 gggggcagta acattgagcc aagtgtccgg agctgcttcc aatttgctaa taataagcca
    12001 gagatcgaag cggccctctt cctagactgg atgagactgg aaccccagtc catggtgtgg
    12061 ctgcccgtcc tgcacagagt ggctgctgca gaaactgcca agcatcaggc caaatgtaac
    12121 atctgcaaag agtgtccaat cattggattc aggtacagga gtctaaagca ctttaattat
    12181 gacatctgcc aaagctgctt tttttctggt cgagttgcaa aaggccataa aatgcactat
    12241 cccatggtgg aatattgcac tccgactaca tcaggagaag atgttcgaga ctttgccaag
    12301 gtactaaaaa acaaatttcg aaccaaaagg tattttgcga agcatccccg aatgggctac
    12361 ctgccagtgc agactgtctt agagggggac aacatggaaa ctcccgttac tctgatcaac
    12421 ttctggccag tagattctgc gcctgcctcg tcccctcagc tttcacacga tgatactcat
    12481 tcacgcattg aacattatgc tagcaggcta gcagaaatgg aaaacagcaa tggatcttat
    12541 ctaaatgata gcatctctcc taatgagagc atagatgatg aacatttgtt aatccagcat
    12601 tactgccaaa gtttgaacca ggactccccc ctgagccagc ctcgtagtcc tgcccagatc
    12661 ttgatttcct tagagagtga ggaaagaggg gagctagaga gaatcctagc agatcttgag
    12721 gaagaaaaca ggaatctgca agcagaatat gaccgtctaa agcagcagca cgaacataaa
    12781 ggcctgtccc cactgccgtc ccctcctgaa atgatgccca cctctcccca gagtccccgg
    12841 gatgctgagc tcattgctga ggccaagcta ctgcgtcaac acaaaggccg cctggaagcc
    12901 aggatgcaaa tcctggaaga ccacaataaa cagctggagt cacagttaca caggctaagg
    12961 cagctgctgg agcaacccca ggcagaggcc aaagtgaatg gcacaacggt gtcctctcct
    13021 tctacctctc tacagaggtc cgacagcagt cagcctatgc tgctccgagt ggttggcagt
    13081 caaacttcgg actccatggg taagtagagg gctgggctgg gctgtggggg gtgtggggtg
    13141 cgggactggg cagtctggga gtccctctca ccacttttct tacctttcta ggtgaggaag
    13201 atcttctcag tcctccccag gacacaagca cagggttaga ggaggtgatg gagcaactca
    13261 acaactcctt ccctagttca agaggaagaa atacccctgg aaagccaatg agagaggaca
    13321 caatgggatc cggtgccacg aacttctctc tgttaaagca agcaggagat gtggaagaaa
    13381 accccggtcc tatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
    13441 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
    13501 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact
    13561 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
    13621 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
    13681 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
    13741 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt
    13801 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg
    13861 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
    13921 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga
    13981 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg
    14041 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
    14101 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
    14161 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc
    14221 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca
    14281 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg
    14341 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca
    14401 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
    14461 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
    14521 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga
    14581 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
    14641 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc
    14701 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca
    14761 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg
    14821 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
    14881 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
    14941 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
    15001 agatcctcat aaaggccaag aagggcggaa agatcgccgt gggatccggt gccacgaact
    15061 tctctctgtt aaagcaagca ggagatgtgg aagaaaaccc cggtcctatg gtgagcaagg
    15121 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
    15181 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
    15241 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
    15301 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
    15361 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
    15421 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
    15481 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
    15541 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
    15601 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
    15661 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
    15721 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
    15781 gccactccac cggcggcatg gacgagctgt acaagtaaat cgataatcaa cctctggatt
    15841 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
    15901 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
    15961 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
    16021 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
    16081 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    16141 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    16201 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
    16261 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    16321 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    16381 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ctcgagccct cgactgtgcc
    16441 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    16501 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    16561 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    16621 caatagcagg catgctgggg atgcggtggg ctctatgggg cgcgcgaaac cggtgaaaaa
    16681 ccgctcagcg gcgcccgcac ccaaacgaaa acacccccgg cccaagcgga ccgggggtgt
    16741 cgcgtgaaat ggatcagtga gcgtggccgt ggtggtggcc gtggccgtgc tcgtcctcgt
    16801 cggccggctt gtcgacgacg gcggtctccg tcgtcaggat catccgggcc accgaggcgg
    16861 cgttgagaac agccgagcgg gtcaccttgg ccggatcgac gacgccggcg gagaccagat
    16921 caccgaactc cagcgtggcg gcgttgaagc cctgcccctt gggaagttcg gagattaatt
    16981 aaccgtttaa attgggcgcg ccaaggaagc tagcaggaca gcaagcgaac cggaattgcc
    17041 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
    17101 gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc
    17161 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    17221 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    17281 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    17341 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    17401 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    17461 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    17521 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    17581 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    17641 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
    17701 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    17761 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    17821 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    17881 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    17941 ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct
    18001 ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc
    18061 gcggaacccc tatttgttta tttttctgcg atcgcataaa tgcttcaata tagcacgtgc
    18121 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    18181 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    18241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    18301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    18361 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    18421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    18481 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    18541 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    18601 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    18661 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    18721 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    18781 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    18841 acgccagcaa cgcggccgct ttttacggtt cctgggcttt tgctggc
//
