LOCUS       sequence-45187-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-B2
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             393..416
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    423..456
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 catcctctag actgccggat ctagctagtt aattaaggat ccgttaacaa gcttcttctt
       61 acattaccat ggtgatttaa tcagaggccg ctgagtcccc tggtttctgc atagttagat
      121 tgtggatgtt tttgagctgc tccttgtaat gtgtctcttg tgttaaggtg catctagtgc
      181 tgttagtgaa gcagcttaca atctactgcc ctaaatgccc cttctcgcac aggctacttc
      241 ttaccttgca tttgaaaagc atatcttgag aaatgatttg aacggcacaa gtttcaaagg
      301 ctctttcagt gtttcgccga gaaacagatg gcggagactg tagtatacag ttgtgtagaa
      361 gaacaagctc ggatgaattc gtatacacta gtgactacaa ggacgacgat gacaagtgat
      421 aaataacttc gtataatgta tgctatacga agttattcta gtatttaaat gccctcgagc
      481 tgactgcggc cgccagcaca gtggtcgacg ataaaataaa agattttatt tagtctccag
      541 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
      601 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
      661 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
      721 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
      781 agcagttcct gccccggctc agggccaaga aca
//
