LOCUS       sequence-451886-        9814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing a tdMCP-protein standard. Used in 
            LABEL-seq abundance assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Simon JJ, Fowler DM, Maly DJ
  TITLE     Multiplexed profiling of intracellular protein abundance, activity, 
            interactions and druggability with LABEL-seq.
  JOURNAL   Nat Methods. 2024 Nov;21(11):2094-2106. doi: 
            10.1038/s41592-024-02456-7. Epub 2024 Oct 21.
  PUBMED    39433876
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-024-02456-7"; journalName: "Nat Methods"; date: 
            "2024-11"; volume: "21"; issue: "11"; pages: "2094-2106"
COMMENT     Sequence Label: 2xMyc-tdMCP-SNAP (tdMCP standard)
FEATURES             Location/Qualifiers
     source          1..9814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      540..549
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             549..578
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             588..617
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1374..1919
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     misc_feature    1987..2104
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2153..2663
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2582..2600
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2678..2687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2684..2686,2687..2689,2690..3403)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2684..2686,2687..2689,2690..3400)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2729..2750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2990..3009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3337..3358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3424..3998,3999..4001,4002..4010)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3591..3608)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3818..3837
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4011..4409
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4481..4714
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4786..4907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4823..4842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4877..4896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4947..5082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5102..5121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5103..5121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5131..5148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5131..5147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5140..5162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5289..5744
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5376..5395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5586..5607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5770..5874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5875..5943,5944..6735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6093..6112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6906..7494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7395..7414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7648..7665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7782..7803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7818..7823,7824..7841,7842..7848)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7856..7872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7861..7883
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7880..7896
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7880..7896
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7915..7935
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7917..7935
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7963..8189
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8190..8370
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8417..8542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9035..9268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9453..9497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(9795..9814,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt aagcttgccg
      541 ccaccatgga gcaaaaactc atcagcgagg aagatcttgg gtcaggagag cagaaattga
      601 tttcagagga agacttgggt tcaggaagcg gttctggagc ctccaatttt actcagttcg
      661 tcttggtgga taacggcggt acgggggacg ttacggtcgc tccctccaat tttgccaacg
      721 gaatagccga gtggatttcc agtaattcca ggtcacaagc gtataaagta acgtgttccg
      781 tcagacaaag ctccgcccaa aatcgaaagt ataccatcaa agtagaagtt ccaaagggtg
      841 cgtggagatc ttatctgaac atggaactca ctattcccat cttcgccacc aactccgact
      901 gtgaacttat cgttaaggca atgcaaggtc tgctgaaaga tggaaaccct atccctagcg
      961 ccatcgccgc aaactccggc atctatgcca tggcttcaaa cttcacccag tttgtgcttg
     1021 tagacaatgg tggcacgggc gacgtgacag tggcaccttc caactttgct aacggcattg
     1081 cagaatggat tagttctaat tcccgctccc aggcttataa agttacgtgt agtgttcgcc
     1141 agtcaagcgc acaaaaccga aagtacacca taaaggttga agtccccaag ggagcctggc
     1201 gatcatacct taacatggag cttactattc cgatcttcgc taccaattca gattgtgaat
     1261 tgattgtcaa agctatgcaa ggcctgctca aggatggtaa cccgatacct agcgctatcg
     1321 ccgccaattc aggcatttat ggcagtggag gttccggatc cggatctggt ggcatggaca
     1381 aagactgcga aatgaagcgc accaccctgg atagccctct gggcaagctg gaactgtctg
     1441 ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa aggaacatct gccgccgacg
     1501 ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc agagccactg atgcaggcca
     1561 ccgcctggct caacgcctac tttcaccagc ctgaggccat cgaggagttc cctgtgccag
     1621 ccctgcacca cccagtgttc cagcaggaga gctttacccg ccaggtgctg tggaaactgc
     1681 tgaaagtggt gaagttcgga gaggtcatca gctacagcca cctggccgcc ctggccggca
     1741 atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg aaatcccgtg cccattctga
     1801 tcccctgcca ccgggtggtg cagggcgacc tggacgtggg gggctacgag ggcgggctcg
     1861 ccgtgaaaga gtggctgctg gcccacgagg gccacagact gggcaagcct gggctgggtt
     1921 gactcgagtc tagagggccc gtttagaatt ctcgacctcg agacaaatgg cagtattcat
     1981 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2041 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2101 ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg
     2161 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     2221 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     2281 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     2341 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     2401 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     2461 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     2521 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     2581 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     2641 gaatcaccga cctctctccc cagggggatc caccggagcc accatggtga gcaagggcga
     2701 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2761 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2821 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2881 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2941 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3001 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3061 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3121 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3181 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3241 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3301 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3361 cgccgccggg atcactctcg gcatggacga gctgtacaag tgactgtgga atcagagaga
     3421 ctgcccctct ccctctcacc cccctaacgt tactggccga agccgcttgg aataaggccg
     3481 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3541 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3601 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3661 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     3721 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     3781 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3841 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     3901 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     3961 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggccaagc
     4021 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     4081 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     4141 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     4201 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     4261 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     4321 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     4381 tgccctctgg ttatgtgtgg gagggctagg gtacctttaa gaccaatgac ttacaaggca
     4441 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4501 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4561 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4681 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     4741 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     4801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     4921 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4981 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     5041 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     5101 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     5161 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5221 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5281 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5341 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5401 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5461 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5521 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5581 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5641 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5701 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     5761 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5821 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5881 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5941 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6001 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6061 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6121 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6181 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6241 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6301 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6361 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6421 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6481 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6541 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6601 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6661 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6721 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6781 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6841 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6901 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7021 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7081 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7141 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7201 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7261 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7321 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7381 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7441 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7501 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7561 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7621 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7681 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7741 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7801 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7861 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     7921 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     7981 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     8041 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     8101 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     8161 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     8221 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8281 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8341 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8401 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8461 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8521 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8581 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8641 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8701 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8761 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8821 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8881 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8941 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9001 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9061 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9121 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9181 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9241 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9301 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9361 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9421 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9481 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9541 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9601 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9661 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9721 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag cctcgagcgg
     9781 ccgccccctt caccgagggc ctatttccca tgat
//
