LOCUS       sequence-451889-        6430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Lyric protein labeled with mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, 
            Voeltz GK
  TITLE     SigmaR1 shapes rough endoplasmic reticulum membrane sheets.
  JOURNAL   Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005.
  PUBMED    38971154
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005."
COMMENT     Sequence Label: Lyric-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..6430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1789..1992)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1818..1838)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1993..2296
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2471..3059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2551..2570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3388..3435
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3460..3479
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3667..4461)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3667..4461)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3778..3797)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4388..4407
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4496..4853)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4510..4645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4662..4853
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4810..4830
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(4855..4959)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4986..5441
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5073..5092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5283..5304
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5448..5569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5459..5478)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5513..5532
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5691..6401)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      6137..6146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6398..6407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtctggcttt tttcttcttt tttatttgtt tgggagattc ccagctagtt tcagacttgg
       61 tctgtgaagg aggcacacta ttttgcttgg tatttgactt ggatttatct gtctcttgta
      121 gtattggcgg cacttgggaa gagctcttgt cagaatcact ttttgataag attacagatg
      181 gctcggtaga agtagcaggt ggaagagtct tgataggctg gctatttttg acgagtactt
      241 cggctggatc actagtgctt atggtcttca aggaaaaagc tttttcctgt tttggacggg
      301 ttttagaggt attcactgga aggtcttctc taatctcttt ttctaattct tctgtgtcct
      361 gtgcagtaga gttatcttca ccttgcttct tctttttctt ttttttcttt ttggatttcc
      421 cagttggaag agctccctct cccttttctt tatcatcatc tgagaccttt tgatcatcag
      481 gaattggttc ctgggacttt agaagtgaag ctctttcttc gtctacccaa ttgccccact
      541 cttctgctgg tgcattccaa tcagagttgg gatcagcaga agacagacca tttaacccag
      601 accattcatc atcgatatag ggttgattac ggctaacatc ccactgataa tcagaagtgg
      661 tagactgaga aactggctca gcagtagacc caatgccaga aaatattgaa cggtcactcc
      721 aactccttcc ccagtctttt ccatttgtat tagcatctcc agtgtcttga ctccaggcag
      781 atggctcagt tttcctcttt cctgcagaag caggtgaaac ggagttccac ttctcctcac
      841 ctgcactgat ctgtgaggag agttttacag acttttcatt ccagcctcct ccattgacag
      901 tgaggttttc attcaatcct gaagaaacct gaagtgtaga atctcctttt ccagatttaa
      961 agttgctaac ttgaacattt agatgagaat cacctttatt cttcttggaa ccaacaggaa
     1021 atgatgcggt tgtaagttgc tcggtggtaa ctgtgatggt attttctatc ccagggatag
     1081 ttgaatccaa tgaaccagaa tcagtcagca ccttatcacg tttacgctgc tgtcgtttct
     1141 ctctgtgact aattttagtt tcccaggctc cttcatcaac ttcctttcca tcatggcgtg
     1201 aactgttttg cactgcttta gcatctgact ttgatttctt cttatttttc tttgactttt
     1261 catttttctt gtcaatctct ggtggctgct ttgctgttac actttgaggt gttcgaacag
     1321 cttcaccctc agccacttca acagtccgcc catttggttt gggcttctcg gacagtttct
     1381 tccggttctt cttcttctgt tcctcgctcc ggagattctt cagcaaggcc aggtcgtcgg
     1441 gggccgcggc cggcacggcc gccgcctcct cccgcttgcg gggcgggctc ctccgctttt
     1501 tgcgggcgcc ggcgcaagcc gcggcccagc cgtagcccag cagaaacagc agcagcagcc
     1561 cgagcgcgcc agtgcccacc aggatcaccc agccggggta ccgtttcggc tccagcccca
     1621 ggtcgaggcc cagctcggtg cgcagaaagc ctaggccgac cgagagcatt tcccgcagcc
     1681 gggccgagcc ctcctcggcc tgctgggcca gctcgtcctg ccagctccgt gcagccatct
     1741 cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag ctctgcttat
     1801 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     1861 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     1921 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     1981 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     2041 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     2101 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     2161 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     2221 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     2281 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     2341 ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca ggggataacg
     2401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3181 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     3241 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     3301 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     3361 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     3421 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     3481 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     3541 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     3601 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     3661 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     3721 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     3781 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     3841 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     3901 ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     3961 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     4021 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     4081 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     4141 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     4201 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     4261 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     4321 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     4381 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     4441 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc
     4501 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     4561 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     4621 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     4681 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     4741 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     4801 actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt
     4861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4981 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5041 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5101 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5161 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5221 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5281 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5341 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5401 atttaacgcg aattttaaca aaatattaac gcttacaatt tacgccttaa gatacattga
     5461 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     5521 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     5581 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     5641 aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc ttacttgtac
     5701 agctcgtcca tgcccatcac atcggtaaag gccttttgcc actccttgaa gttgagctcg
     5761 gtcttggagt gcttgagctc cgtcttacgg aacacgtaca tcggctggtt cttcagatag
     5821 ttagccgcca ttggcttggc aaaggtgtag gtggtccgcg cagtgctccg gtagcgcttg
     5881 ccatttccag tggtgtaact ccacttaaag gtactgatga tggttttgtc gttggggtaa
     5941 gtcttcttcg acctgcacca gtccgcagcg gtcagcgagt tggtcatcac aggaccgtca
     6001 gcagggaaac cagtcccctt cacctgggcc tctcctttga tgtggcttcc ctcgtaggtg
     6061 tagcggtagt taacagtaag ggaggcacca tcttcaaact gcattgtgcg atggacttgg
     6121 tagccggagc catctaccat ggcggcctgg aaaggcgaca tcccgtcagg gtagggcagg
     6181 tactgatgga agccataccc gatatgaggg accagaatcc agggggagaa ctggaggtca
     6241 cccttggtgg acttcaggtt taactcctca taaccatcat ttggattgcc ggtgccctga
     6301 cccaccatgt caaagtccac accgttgatg gagccaaaga tgtgtaactc atgtgtcgct
     6361 gggagagagg ccatgttatc ctcctcgccc ttgctcacca tggtggcgac cggtggatcc
     6421 cgcgtttctc
//
