LOCUS       sequence-45189-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-B3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             373..396
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    403..436
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 catcctctag actgccggat ctagctagtt aattaaggat ccgttaacaa gctttacttc
       61 ttaccttgca tttgaaaagc atatcttgag aaatgatttg aacggcacaa gtttcaaagg
      121 ctctttcagt gtttcgccga gaaacagatg gcggagactg tagtatacag ttgtgtagaa
      181 gaacaagctc ggatcctagt agtgccaaag tgctcatagt gcaggtagtt tttataccac
      241 tctactgcag tgtgagcact tctagtactc ctggatcaac caaggtcttg ttgaagcaac
      301 tacagcgcaa ggacattgct acttacgatt agttttgcag atttgcagtt cagcgaattc
      361 gtatacacta gtgactacaa ggacgacgat gacaagtgat aaataacttc gtataatgta
      421 tgctatacga agttattcta gtatttaaat gccctcgagc tgactgcggc cgccagcaca
      481 gtggtcgacg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
      541 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
      601 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
      661 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
      721 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      781 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaaacc
      841 atcag
//
