LOCUS       sequence-451890-        6430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Lyric protein labeled with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, 
            Voeltz GK
  TITLE     SigmaR1 shapes rough endoplasmic reticulum membrane sheets.
  JOURNAL   Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005.
  PUBMED    38971154
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005."
COMMENT     Sequence Label: Lyric-mCherry
FEATURES             Location/Qualifiers
     source          1..6430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1663..1672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1669..2379
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1814..1832)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2082..2101
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2501..2622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2538..2557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2592..2611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2629..3084)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2766..2787)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2978..2997
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3111..3215
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3217..3574
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3217..3408
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3240..3260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3425..3560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3609..4403
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3609..4403
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3663..3682)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4273..4292
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4591..4610)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4635..4682
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5011..5599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5500..5519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5774..6077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6078..6281
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6232..6252
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 accgagctgg gcctcgacct ggggctggag ccgaaacggt accccggctg ggtgatcctg
       61 gtgggcactg gcgcgctcgg gctgctgctg ctgtttctgc tgggctacgg ctgggccgcg
      121 gcttgcgccg gcgcccgcaa aaagcggagg agcccgcccc gcaagcggga ggaggcggcg
      181 gccgtgccgg ccgcggcccc cgacgacctg gccttgctga agaatctccg gagcgaggaa
      241 cagaagaaga agaaccggaa gaaactgtcc gagaagccca aaccaaatgg gcggactgtt
      301 gaagtggctg agggtgaagc tgttcgaaca cctcaaagtg taacagcaaa gcagccacca
      361 gagattgaca agaaaaatga aaagtcaaag aaaaataaga agaaatcaaa gtcagatgct
      421 aaagcagtgc aaaacagttc acgccatgat ggaaaggaag ttgatgaagg agcctgggaa
      481 actaaaatta gtcacagaga gaaacgacag cagcgtaaac gtgataaggt gctgactgat
      541 tctggttcat tggattcaac tatccctggg atagaaaata ccatcacagt taccaccgag
      601 caacttacaa ccgcatcatt tcctgttggt tccaagaaga ataaaggtga ttctcatcta
      661 aatgttcaag ttagcaactt taaatctgga aaaggagatt ctacacttca ggtttcttca
      721 ggattgaatg aaaacctcac tgtcaatgga ggaggctgga atgaaaagtc tgtaaaactc
      781 tcctcacaga tcagtgcagg tgaggagaag tggaactccg tttcacctgc ttctgcagga
      841 aagaggaaaa ctgagccatc tgcctggagt caagacactg gagatgctaa tacaaatgga
      901 aaagactggg gaaggagttg gagtgaccgt tcaatatttt ctggcattgg gtctactgct
      961 gagccagttt ctcagtctac cacttctgat tatcagtggg atgttagccg taatcaaccc
     1021 tatatcgatg atgaatggtc tgggttaaat ggtctgtctt ctgctgatcc caactctgat
     1081 tggaatgcac cagcagaaga gtggggcaat tgggtagacg aagaaagagc ttcacttcta
     1141 aagtcccagg aaccaattcc tgatgatcaa aaggtctcag atgatgataa agaaaaggga
     1201 gagggagctc ttccaactgg gaaatccaaa aagaaaaaaa agaaaaagaa gaagcaaggt
     1261 gaagataact ctactgcaca ggacacagaa gaattagaaa aagagattag agaagacctt
     1321 ccagtgaata cctctaaaac ccgtccaaaa caggaaaaag ctttttcctt gaagaccata
     1381 agcactagtg atccagccga agtactcgtc aaaaatagcc agcctatcaa gactcttcca
     1441 cctgctactt ctaccgagcc atctgtaatc ttatcaaaaa gtgattctga caagagctct
     1501 tcccaagtgc cgccaatact acaagagaca gataaatcca agtcaaatac caagcaaaat
     1561 agtgtgcctc cttcacagac caagtctgaa actagctggg aatctcccaa acaaataaaa
     1621 aagaagaaaa aagccagacg agaaacgcgg gatccaccgg tcgccaccat ggtgagcaag
     1681 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1741 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1801 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1861 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1921 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1981 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2041 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2101 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2161 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2221 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     2281 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2341 cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     2401 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2461 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2521 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2581 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     2641 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2701 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     2761 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2821 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     2881 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2941 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3001 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3061 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3121 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3181 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     3241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3481 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3541 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     3601 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3661 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3721 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3781 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3841 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3901 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3961 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4021 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4081 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4141 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4201 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4261 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4321 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4381 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     4441 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4501 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4561 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     4621 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     4681 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     4741 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     4801 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     4861 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4921 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5701 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     5761 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5821 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5881 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     5941 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6001 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6061 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     6121 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     6181 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     6241 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     6301 tccgctagcg ctaccggact cagatctcga gatggctgca cggagctggc aggacgagct
     6361 ggcccagcag gccgaggagg gctcggcccg gctgcgggaa atgctctcgg tcggcctagg
     6421 ctttctgcgc
//
