LOCUS       sequence-451893-        5638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Lrrc59 protein labeled with mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, 
            Voeltz GK
  TITLE     SigmaR1 shapes rough endoplasmic reticulum membrane sheets.
  JOURNAL   Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005.
  PUBMED    38971154
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005."
COMMENT     Sequence Label: mNeonGreen-Lrrc59
FEATURES             Location/Qualifiers
     source          1..5638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(641..1348)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1084..1093
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1345..1354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1393..1596)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1422..1442)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1597..1900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2075..2663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2155..2174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2992..3039
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3064..3083
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3271..4065)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3271..4065)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3382..3401)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3992..4011
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4100..4457)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4114..4249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4266..4457
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4414..4434
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(4459..4563)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4590..5045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4677..4696)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4887..4908
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5052..5173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5063..5082)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5117..5136
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgagggcatc atactccttt ctccggcgct ccttctcttc cgccttctcc cgcttccgca
       61 gttcccgctc ctgagcttcc ttagctcgct gcttagcctc acgcttcttc tctgcctcac
      121 gttctacttc cagccgccgc tgcctctccc gctcctgatc tgcctgcacg gccttcatgt
      181 gctgtaacac cttgtttgca cactgcttac actgcttctc atccaagcag tcacctgcca
      241 ccttggccag gacaggatcc agggggttat ccttcaggtc caaccacttc aggttcttga
      301 gctgagcaaa gctgacaggc aaggtgacca gcttgttgtt gaggagatcc aggtgctgga
      361 ggttgaccag acggccaaag tctgctggca gctgctgcag cttgttctta ctcaggtcta
      421 gcttcaccag gtgtgtgagg ccacagaaat ccgacggtag agtagtcagt ttattacaag
      481 acagatccag gatggtggcc tttggaaggg cagccagctc cttcaccggg acctcattca
      541 ggtcgctgag gctcaggtcc agttcgttgc cgtccagctt gtcgcggagg ttcccgccct
      601 tgctaccggc cttggtcata gctcgagatc tgagtccgga cttgtacagc tcgtccatgc
      661 ccatcacatc ggtaaaggcc ttttgccact ccttgaagtt gagctcggtc ttggagtgct
      721 tgagctccgt cttacggaac acgtacatcg gctggttctt cagatagtta gccgccattg
      781 gcttggcaaa ggtgtaggtg gtccgcgcag tgctccggta gcgcttgcca tttccagtgg
      841 tgtaactcca cttaaaggta ctgatgatgg ttttgtcgtt ggggtaagtc ttcttcgacc
      901 tgcaccagtc cgcagcggtc agcgagttgg tcatcacagg accgtcagca gggaaaccag
      961 tccccttcac ctgggcctct cctttgatgt ggcttccctc gtaggtgtag cggtagttaa
     1021 cagtaaggga ggcaccatct tcaaactgca ttgtgcgatg gacttggtag ccggagccat
     1081 ctaccatggc ggcctggaaa ggcgacatcc cgtcagggta gggcaggtac tgatggaagc
     1141 catacccgat atgagggacc agaatccagg gggagaactg gaggtcaccc ttggtggact
     1201 tcaggtttaa ctcctcataa ccatcatttg gattgccggt gccctgaccc accatgtcaa
     1261 agtccacacc gttgatggag ccaaagatgt gtaactcatg tgtcgctggg agagaggcca
     1321 tgttatcctc ctcgcccttg ctcaccatgg tggcgaccgg tagcgctagc ggatctgacg
     1381 gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     1441 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     1501 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     1561 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     1621 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     1681 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     1741 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     1801 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     1861 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     1921 atgaactaat gaccccgtaa ttgattacta ttaataacta atgcatggcg gtaatacggt
     1981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac
     2821 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     2881 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     2941 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     3001 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     3061 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca
     3121 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     3181 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     3241 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3301 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3361 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3421 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3481 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc
     3541 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     3601 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     3661 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     3721 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3781 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3841 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc
     3901 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     3961 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     4021 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     4081 tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     4141 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     4201 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4261 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4321 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4381 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     4441 ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4561 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     4621 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4681 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4741 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4801 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4861 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4921 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4981 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5041 aatttacgcg ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     5101 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     5161 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     5221 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     5281 gttatctaga tccggtggat cccgggcccg cggtaccgtc gactgcagaa ttctcactgc
     5341 tgagagtcgg tctggaggac ccactggagg atctcatggc ggcgtagacc ctggaccgca
     5401 ttgtcataga tggtgttcac gctggtgcag aggggctgct gctgcagctc tgtcacccga
     5461 caagcaacca gccctcccgc cacaccaaat agcagcagca gcagcagcag cttcagcaca
     5521 gcccaggaac gagtgtgctt ccggggtggt ggcttgcggg gacgggagcc agacttagat
     5581 ttcggggcct gatttgcttc cttcttaggt ttcttctcct gctcccgctt ggctgctt
//
