LOCUS       sequence-451894-        5638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Lrrc59 protein laebeled with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, 
            Voeltz GK
  TITLE     SigmaR1 shapes rough endoplasmic reticulum membrane sheets.
  JOURNAL   Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005.
  PUBMED    38971154
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005."
COMMENT     Sequence Label: mCherry-Lrrc59
FEATURES             Location/Qualifiers
     source          1..5638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..83
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(172..529)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      186..321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        338..529
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     486..506
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(531..635)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      662..1117
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(749..768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     959..980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1124..1245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1135..1154)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1189..1208
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2351..3058)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2626..2645)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2895..2913
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3055..3064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3103..3306)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3132..3152)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3307..3610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3785..4373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3865..3884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4702..4749
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4774..4793
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(4981..5638,1..137))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(join(4981..5638,1..137))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5092..5111)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
       61 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
      121 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
      181 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
      241 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      301 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      361 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
      421 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
      481 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
      541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      661 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      841 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      961 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     1021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     1081 aacgcgaatt ttaacaaaat attaacgctt acaatttacg cgttaagata cattgatgag
     1141 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     1201 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1261 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     1321 ctctacaaat gtggtatggc tgattatgat cagttatcta gatccggtgg atcccgggcc
     1381 cgcggtaccg tcgactgcag aattctcact gctgagagtc ggtctggagg acccactgga
     1441 ggatctcatg gcggcgtaga ccctggaccg cattgtcata gatggtgttc acgctggtgc
     1501 agaggggctg ctgctgcagc tctgtcaccc gacaagcaac cagccctccc gccacaccaa
     1561 atagcagcag cagcagcagc agcttcagca cagcccagga acgagtgtgc ttccggggtg
     1621 gtggcttgcg gggacgggag ccagacttag atttcggggc ctgatttgct tccttcttag
     1681 gtttcttctc ctgctcccgc ttggctgctt tgagggcatc atactccttt ctccggcgct
     1741 ccttctcttc cgccttctcc cgcttccgca gttcccgctc ctgagcttcc ttagctcgct
     1801 gcttagcctc acgcttcttc tctgcctcac gttctacttc cagccgccgc tgcctctccc
     1861 gctcctgatc tgcctgcacg gccttcatgt gctgtaacac cttgtttgca cactgcttac
     1921 actgcttctc atccaagcag tcacctgcca ccttggccag gacaggatcc agggggttat
     1981 ccttcaggtc caaccacttc aggttcttga gctgagcaaa gctgacaggc aaggtgacca
     2041 gcttgttgtt gaggagatcc aggtgctgga ggttgaccag acggccaaag tctgctggca
     2101 gctgctgcag cttgttctta ctcaggtcta gcttcaccag gtgtgtgagg ccacagaaat
     2161 ccgacggtag agtagtcagt ttattacaag acagatccag gatggtggcc tttggaaggg
     2221 cagccagctc cttcaccggg acctcattca ggtcgctgag gctcaggtcc agttcgttgc
     2281 cgtccagctt gtcgcggagg ttcccgccct tgctaccggc cttggtcata gctcgagatc
     2341 tgagtccgga cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt
     2401 cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga
     2461 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag
     2521 cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc
     2581 cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta
     2641 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
     2701 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc
     2761 gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt
     2821 gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg
     2881 gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
     2941 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
     3001 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatgg
     3061 tggcgaccgg tagcgctagc ggatctgacg gttcactaaa ccagctctgc ttatatagac
     3121 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     3181 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     3241 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     3301 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     3361 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     3421 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     3481 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     3541 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     3601 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     3661 ttaataacta atgcatggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4201 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4501 ttaaatcaat ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga
     4561 cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag
     4621 aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct
     4681 gggaccgaac cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt
     4741 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4801 tccccctagg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     4861 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     4921 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     4981 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     5041 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     5101 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     5161 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     5221 gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc
     5281 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     5341 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     5401 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     5461 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     5521 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     5581 cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaaga
//
