LOCUS       sequence-451897-        4727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mNeonGreen with KDEL ER lumenal target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Sawyer EM, Jensen LE, Meehl JB, Larsen KP, Petito DA, Hurley JH, 
            Voeltz GK
  TITLE     SigmaR1 shapes rough endoplasmic reticulum membrane sheets.
  JOURNAL   Dev Cell. 2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005.
  PUBMED    38971154
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2024 Jul 2:S1534-5807(24)00382-4. doi: 
            10.1016/j.devcel.2024.06.005."
COMMENT     Sequence Label: mNeonGreen-KDEL
FEATURES             Location/Qualifiers
     source          1..4727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(151..855)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      594..603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(938..1141)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(967..987)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1142..1445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1620..2208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1700..1719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2537..2584
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2609..2628
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2816..3610)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2816..3610)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2927..2946)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3537..3556
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3645..4002)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3659..3794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3811..4002
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     3959..3979
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(4004..4108)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4135..4590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4222..4241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4432..4453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4597..4718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4608..4627)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4662..4681
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
       61 taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagatc cggtggatcc
      121 cgggcccgcg gccgctcata gctcgtcttt cttgtacagc tcgtccatgc ccatcacatc
      181 ggtaaaggcc ttttgccact ccttgaagtt gagctcggtc ttggagtgct tgagctccgt
      241 cttacggaac acgtacatcg gctggttctt cagatagtta gccgccattg gcttggcaaa
      301 ggtgtaggtg gtccgcgcag tgctccggta gcgcttgcca tttccagtgg tgtaactcca
      361 cttaaaggta ctgatgatgg ttttgtcgtt ggggtaagtc ttcttcgacc tgcaccagtc
      421 cgcagcggtc agcgagttgg tcatcacagg accgtcagca gggaaaccag tccccttcac
      481 ctgggcctct cctttgatgt ggcttccctc gtaggtgtag cggtagttaa cagtaaggga
      541 ggcaccatct tcaaactgca ttgtgcgatg gacttggtag ccggagccat ctaccatggc
      601 ggcctggaaa ggcgacatcc cgtcagggta gggcaggtac tgatggaagc catacccgat
      661 atgagggacc agaatccagg gggagaactg gaggtcaccc ttggtggact tcaggtttaa
      721 ctcctcataa ccatcatttg gattgccggt gccctgaccc accatgtcaa agtccacacc
      781 gttgatggag ccaaagatgt gtaactcatg tgtcgctggg agagaggcca tgttatcctc
      841 ctcgcccttg ctcacggccc gcgccgcgct gagcagcagc agcatcgcgg ccaccaggga
      901 gagcttcatg ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc
      961 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     1021 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     1081 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     1141 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     1201 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     1261 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     1321 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     1381 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     1441 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     1501 aactaatgca tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2341 tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg
     2401 gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg
     2461 cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac
     2521 cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt
     2581 gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc
     2641 ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     2701 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     2761 cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga
     2821 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     2881 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     2941 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     3001 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     3061 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     3121 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     3181 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     3241 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     3301 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     3361 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     3421 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     3481 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     3541 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     3601 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca aaagcctagg
     3661 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     3721 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     3781 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     3841 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     3901 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     3961 cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc ctttttcaat
     4021 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4081 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4561 attttaacaa aatattaacg cttacaattt acgcgttaag atacattgat gagtttggac
     4621 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4681 ctttatttgt aaccattata agctgcaata aacaagttaa caacaac
//
