LOCUS       sequence-451898-        5313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Rab1b protein labeled with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Westrate LM, Hoyer MJ, Nash MJ, Voeltz GK
  TITLE     Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER 
            to Golgi transport.
  JOURNAL   J Cell Sci. 2020 Jul 24;133(14). pii: jcs.239814. doi: 
            10.1242/jcs.239814.
  PUBMED    32616562
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-07-24"; volume: "133(14). pii: jcs.239814. doi"; 
            pages: " 10.1242/jcs.239814"
COMMENT     Sequence Label: GFP-Rab1b
FEATURES             Location/Qualifiers
     source          1..5313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(464..1052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(544..563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1381..1428
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1453..1472
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1660..2454)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1660..2454)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1771..1790)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2381..2400
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2489..2846)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2503..2638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2655..2846
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2803..2823
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(2848..2952)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2979..3434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3066..3085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3276..3297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3441..3562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3452..3471)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3506..3525
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4334..5050)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     regulatory      5047..5056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5095..5298)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5124..5144)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(5299..5313,1..289)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
       61 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
      121 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
      181 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
      241 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
      301 atatgggcta tgaactaatg accccgtaat tgattactat taataactaa tgcatggcgg
      361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1201 atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     1261 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     1321 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     1381 gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg
     1441 ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga
     1501 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     1561 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     1621 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     1681 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     1741 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     1801 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     1861 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     1921 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     1981 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     2041 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     2101 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     2161 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     2221 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     2281 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     2341 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     2401 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     2461 cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc
     2521 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     2581 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     2641 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     2701 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     2761 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     2821 tccacagctg gttctttccg cctcaggact cttccttttt caatattatt gaagcattta
     2881 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2941 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     3001 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3061 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3121 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3181 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3241 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3301 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3361 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3421 aacgcttaca atttacgcgt taagatacat tgatgagttt ggacaaacca caactagaat
     3481 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3541 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3601 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     3661 ttatgatcag ttatctagat ccggtggatc ccgggcccgc ggtaccctag caacagccac
     3721 cgccagccgg ctttacaggg gtgctgtcga tcttgagatt gggccgctcg cccccagagg
     3781 ctgctccagg ccccatccgc tttttgattt cagcagccat ggtcatgaac gcctgctcga
     3841 cattggtggc attcttggcg ctcgtctcca agaaggggat gcccagagag tctgcaaact
     3901 ccttggctgt ggtgttgtcc accaccttct tggtggtgag gtcgctcttg ttgcccacca
     3961 ggagcttatt gacgttctcg ctggcatagc ggtcaatctc ctgcagccac tgcttcacgt
     4021 tggcgtagga ttcctggtca gtgacgtcat acaccacgat gatgccatga gccccccggt
     4081 agtagctgga agtgatggtc cggaaccgtt cctggcccgc tgtgtcccag atctgaagtt
     4141 tgatagtttt gccatccagc tcgatggttc ggatcttgaa gtccaccccg atggtgctga
     4201 tgtagctctc tgtgtacgtg tcatcagcaa accgcaggag caggcatgac ttgcccacgc
     4261 ctgagtcgcc aatcaaaagc agcttaaaca ggtagtcata ttcggggttc atagctcgag
     4321 atctgagtcc ggacttgtac agctcatcca tgccgtgggt gatggcggcg gcggtcacga
     4381 agccgaagta gatcatgtga tcgcgcttct cgttggggtc cttggacagg gcgctctggg
     4441 tggacaggta gtggttatcg ggcagcagca cagggccatc gccgatgggg gtattctgct
     4501 ggtagtggtc ggccagctgc acgctgccat cctcgatgtt gtggcggatc ttgaagttca
     4561 ccttgatgcc attcttggcc ttgtcggtca tgatgtacac attgtgggcg ttgtagttgt
     4621 actccatctt attgcccagg atgttgccat cctccttgaa atcggtgccg gtcagctcga
     4681 tgcgattcac cagggtatcg ccctcgaact tcacctcggc gcgcgacttg tagttgccgt
     4741 catcctcgaa gaagatggtg cgctcctgga tgtagccctc aggcatggcg ctcttgaaga
     4801 agtcgtgctg cttcatgtga tcggggtagc gtgagaagca ctgcacgccg tagctcaggg
     4861 tggtcaccag ggtgggccag ggcacaggca gcttgccggt ggtgcagatg aacttcaggg
     4921 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgct cacgctgaac ttgtggccat
     4981 tcacatcgcc attcagctcg atcaggatgg gcacgatgcc ggtgaacagc tcggcgccct
     5041 tgctcaccat ggtggcgacc ggtagcgcta gcggatctga cggttcacta aaccagctct
     5101 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     5161 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     5221 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     5281 ctgccaaaac cgcatcacca tggtaatagc gat
//
