LOCUS       sequence-45190-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-B4
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..380
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    387..420
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggaccatcct ctagactgcc ggatctagct agttaattaa ggatccgtta acaagcttgt
       61 gcaaatccat gcaaaactga ttgtgggaat gtgtaccttt ctgcatcgta atggacacct
      121 ttatgcacat cttcagcatc catgcccatt catccacagg tggggattgg tggcattact
      181 tgtgttagat ataaagtatt gcacttgtcc cggcctgagg aagaaagagg gtttttaatc
      241 gtcttttagt ttctgagtat tgtaagttat gttagactgt aattatgaaa tgaactgttt
      301 tgctgttatc aggtggaaca cgatgcaatt ttggttggga attcgtatac actagtgact
      361 acaaggacga cgatgacaag tgataaataa cttcgtataa tgtatgctat acgaagttat
      421 tctagtattt aaatgccctc gagctgactg cggccgccag cacagtggtc gacgataaaa
      481 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
      541 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
      601 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
      661 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      721 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      781 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      841 tgccccaagg acctgaaatg accctgtgcc ttattt
//
