LOCUS       sequence-451907-        9771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of 
            human full-length GSDMD fusion with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Fontana P, Du G, Zhang Y, Zhang H, Vora SM, Hu JJ, Shi M, Tufan AB, 
            Healy LB, Xia S, Lee DJ, Li Z, Baldominos P, Ru H, Luo HR, Agudo J, 
            Lieberman J, Wu H
  TITLE     Small-molecule GSDMD agonism in tumors stimulates antitumor immunity
            without toxicity.
  JOURNAL   Cell. 2024 Sep 2:S0092-8674(24)00898-5. doi: 
            10.1016/j.cell.2024.08.007.
  PUBMED    39243763
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Sep 2:S0092-8674(24)00898-5. doi: 10.1016/j.cell.2024.08.007."
COMMENT     Sequence Label: pLenti-CMV-GSDMD-eGFP-SV40p-BlasR
FEATURES             Location/Qualifiers
     source          1..9771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(164..169,170..187,188..194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     226..242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     261..281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        263..281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        309..535
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             536..716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    763..888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1381..1614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1799..1843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2110..2227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2250..2553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2554..2757
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2708..2728
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2754..2778
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2783..2792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4241..4243,4244..4954)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4241..4243,4244..4954)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4283..4304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4544..4563)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4891..4912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5015..5056
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5080..5668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5133..5153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5551..5562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5683..5999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5850..5985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6047..6094
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6113..6511
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6598..6831
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6903..7024
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6940..6959)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6994..7013
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7064..7199
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7219..7238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7220..7238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7248..7265)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7248..7264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7257..7279)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7406..7861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7493..7512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7703..7724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7887..7991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7992..8060,8061..8852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8210..8229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9023..9611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9512..9531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9765..9771,1..11)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
       61 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
      301 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
      361 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      421 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      481 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      721 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      781 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      841 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      901 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      961 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1021 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1081 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1141 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1201 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1261 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1321 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1381 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1441 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1501 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1561 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1621 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1681 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1741 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1801 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1861 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1921 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1981 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2041 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2101 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2161 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2221 aaattttatc gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2281 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2341 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2401 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2461 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2521 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2581 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2641 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2701 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     2761 ttagtgaacc gtcagatcgc ctgccaccat ggggtcggcc tttgagcggg tagtccggag
     2821 agtggtccag gagctggacc atggtgggga gttcatccct gtgaccagcc tgcagagctc
     2881 cactggcttc cagccctact gcctggtggt taggaagccc tcaagctcat ggttctggaa
     2941 accccgttat aagtgtgtca acctgtctat caaggacatc ctggagccgg atgccgcgga
     3001 accagacgtg cagcgtggca ggagcttcca cttctacgat gccatggatg ggcagataca
     3061 gggcagcgtg gagctggcag ccccaggaca ggcaaagatc gcaggcgggg ccgcggtgtc
     3121 tgacagctcc agcacctcaa tgaatgtgta ctcgctgagt gtggacccta acacctggca
     3181 gactctgctc catgagaggc acctgcggca gccagaacac aaagtcctgc agcagctgcg
     3241 cagccgcggg gacaacgtgt acgtggtgac tgaggtgctg cagacacaga aggaggtgga
     3301 agtcacgcgc acccacaagc gggagggctc gggccggttt tccctgcccg gagccacgtg
     3361 cttgcagggt gagggccagg gccatctgag ccagaagaag acggtcacca tcccctcagg
     3421 cagcaccctc gcattccggg tggcccagct ggttattgac tctgacttgg acgtccttct
     3481 cttcccggat aagaagcaga ggaccttcca gccacccgcg acaggccaca agcgttccac
     3541 gagcgaaggc gcctggccac agctgccctc tggcctctcc atgatgaggt gcctccacaa
     3601 cttcctgaca gatggggtcc ctgcggaggg ggcgttcact gaagacttcc agggcctacg
     3661 ggcagaggtg gagaccatct ccaaggaact ggagcttttg gacagagagc tgtgccagct
     3721 gctgctggag ggcctggagg gggtgctgcg ggaccagctg gccctgcgag ccttggagga
     3781 ggcgctggag cagggccaga gccttgggcc ggtggagccc ctggacggtc cagcaggtgc
     3841 tgtcctggag tgcctggtgt tgtcctccgg aatgctggtg ccggaactcg ctatccctgt
     3901 tgtctacctg ctgggggcac tgaccatgct gagtgaaacg cagcacaagc tgctggcgga
     3961 ggcgctggag tcgcagaccc tgttggggcc gctcgagctg gtgggcagcc tcttggagca
     4021 gagtgccccg tggcaggagc gcagcaccat gtccctgccc cccgggctcc tggggaacag
     4081 ctggggcgaa ggagcaccgg cctgggtctt gctggacgag tgtggcctag agctggggga
     4141 ggacactccc cacgtgtgct gggagccgca ggcccagggc cgcatgtgtg cactctacgc
     4201 ctccctggca ctgctatcag gactgagcca ggagccccac gtgagcaagg gcgaggagct
     4261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     4441 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4681 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4921 cgggatcact ctcggcatgg acgagctgta caaggctagc gtgtgatcag aattcgttcc
     4981 ggagtcgtcg actctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     5041 cggtctcgat tctacgcgta ccggttagta atgatcgaca atcaacctct ggattacaaa
     5101 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5161 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5221 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5281 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5341 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5401 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5461 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     5521 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5581 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5641 cggatctccc tttgggccgc ctccccgcct ggcgatggta ccggtgtgga aagtccccag
     5701 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5761 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5821 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5881 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     5941 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6001 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     6061 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     6121 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     6181 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     6241 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     6301 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     6361 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     6421 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     6481 gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac
     6541 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6601 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6661 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6721 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6781 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6841 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6901 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7021 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7081 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7141 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7201 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     7261 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7321 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7381 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7621 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7681 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7741 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7801 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7861 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7921 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7981 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8041 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8101 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8161 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8221 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8281 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8341 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8401 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8461 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8521 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8581 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8641 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8701 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8761 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8821 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8881 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8941 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9001 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9061 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag t
//
