LOCUS       sequence-451920-       11020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone plasmid to express TurboID fusion proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Gomes-Junior R, Moreira CMDN, Dallagiovanna B
  TITLE     Construction of a proximity labeling vector to identify 
            protein-protein interactions in human stem cells.
  JOURNAL   PLoS One. 2025 May 30;20(5):e0324779. doi: 
            10.1371/journal.pone.0324779. eCollection 2025.
  PUBMED    40445938
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2025-05-30"; volume: "20(5):e0324779. doi"; pages: " 
            10.1371/journal.pone.0324779. eCollection 2025"
COMMENT     Sequence Label: AAVS1-TRE-TurboID-HA-rtTA
FEATURES             Location/Qualifiers
     source          1..11020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1105..1130
                     /label=SA
                     /note="splice acceptor site"
     CDS             1154..1207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1217..1816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1217..1236)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1713..1733
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1848..1865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1854..2078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2153..2171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2190..2208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2227..2245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2264..2282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2301..2319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2338..2356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2375..2393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2415..2439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3503..3529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3556..3677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        3838..4141
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4415..5431
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5355..5377
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5439..5458
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(5493..6110,6111..6116,6117..6152,6153..6155,6156..6191,
                     6192..6194,6195..6230,6231..6239)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    6266..6387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6522..7358
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7392..7411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7393..7411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7418..7435)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7418..7434)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7427..7449)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7572..7874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7753..7772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8223..9017
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8223..9017
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8277..8296)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8887..8906
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9267..10058,10059..10127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9890..9909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10251..10839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10740..10759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10993..11010
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
     1141 atctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2041 caatagcagg catgctgggg atgcggtggg ctctatgggt cgacgacgcg tccccgaaaa
     2101 gtgccacctg acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc
     2161 agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc
     2221 tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga
     2281 gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc
     2341 ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacc
     2401 ccctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2461 tgttttgacc tccatagaag acaccgggac cgatccagcc taccggtcct cagcggccca
     2521 catggccctt aagttcgaaa aggacaacac ggtgcccctc aagctcattg cactcctcgc
     2581 aaacggagag ttccacagcg gagagcaact cggagagacg ctcggaatga gccgcgcagc
     2641 aattaacaag cacattcaaa cgctccgcga ctggggagtg gacgtgttca cggtgcccgg
     2701 aaagggatac agcctccccg agcccattcc cctcctcaac gcaaagcaaa ttctcggaca
     2761 actcgacgga ggaagcgtgg cagtgctccc cgtggtggac agcacgaacc aatacctcct
     2821 cgaccgcatt ggagagctca agagcggaga cgcatgcatt gcagagtacc aacaagcagg
     2881 acgcggaagc cgcggacgca agtggttcag ccccttcgga gcaaacctct acctcagcat
     2941 gttctggcgc ctcaagcgcg gacccgcagc aattggactc ggacccgtga ttggaattgt
     3001 gatggcagag gcactccgca agctcggagc agacaaggtg cgcgtgaagt ggcccaacga
     3061 cctctacctc caagaccgca agctcgcagg tattctcgtg gagctcgcag gaattacggg
     3121 agacgcagca caaattgtga ttggagcagg aattaacgtg gcaatgcgcc gcgtggagga
     3181 gagcgtggtg aaccaaggat ggattacgct ccaagaggca ggaattaacc tcgaccgcaa
     3241 cacgctcgca gcaacgctca ttcgcgagct ccgcgcagca ctcgagctct tcgagcaaga
     3301 gggactcgca ccctacctcc cccgctggga gaagctcgac aacttcatta accgccccgt
     3361 gaagctcatt attggagaca aggagatttt cggaattagc cgcggaattg acaagcaagg
     3421 agcactcctc ctcgagcaag acggagtgat taagccctgg atgggaggag agattagcct
     3481 ccgcagcgca gagaaggcaa gctaccccta cgacgtgccc gactacgcat aattaattaa
     3541 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3601 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3661 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3721 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtagg cgcgccctag
     3781 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3841 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3901 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3961 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     4021 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     4081 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4141 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     4201 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     4261 ggggsgcgcg ccaggggggg gggggggggg ggaggggggg gggggggsga ggsggagagg
     4321 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     4381 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
     4441 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     4501 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     4561 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     4621 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     4681 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     4741 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     4801 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     4861 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     4921 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     4981 ggggtggcgg caggkggggg tgccgggcgg ggcggggccg ccycgggccg gggggggckc
     5041 gggggrgggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     5101 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     5161 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     5221 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     5281 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     5341 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     5401 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     5461 gtctcatcat tttggcaaag aattccgcca ccatgtctag actggacaag agcaaagtca
     5521 taaacggcgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
     5581 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     5641 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     5701 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc cgctgtgctc
     5761 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     5821 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     5881 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     5941 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     6001 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     6061 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccggccg
     6121 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     6181 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaag
     6241 atatcgccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     6301 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6361 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6421 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     6481 atcgcgataa ggatccgggc tggtcgacag tactaagctt tactagggac aggattggtg
     6541 acagaaaagc cccatcctta ggcctcctcc ttcctagtct cctgatattg ggtctaaccc
     6601 ccacctcctg ttaggcagat tccttatctg gtgacacacc cccatttcct ggagccatct
     6661 ctctccttgc cagaacctct aaggtttgct tacgatggag ccagagagga tcctgggagg
     6721 gagagcttgg cagggggtgg gagggaaggg ggggatgcgt gacctgcccg gttctcagtg
     6781 gccaccctgc gctaccctct cccagaacct gagctgctct gacgcggctg tctggtgcgt
     6841 ttcactgatc ctggtgctgc agcttcctta cacttcccaa gaggagaagc agtttggaaa
     6901 aacaaaatca gaataagttg gtcctgagtt ctaactttgg ctcttcacct ttctagtccc
     6961 caatttatat tgttcctccg tgcgtcagtt ttacctgtga gataaggcca gtagccagcc
     7021 ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag
     7081 aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc tactcccttt ctctttctcc
     7141 atccttcttt ccttaaagag tccccagtgc tatctgggac atattcctcc gcccagagca
     7201 gggtcccgct tccctaaggc cctgctctgg gcttctgggt ttgagtcctt ggcaagccca
     7261 ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg cccctggctc
     7321 tcctgcccct tccctacagg ggttcctggc tctgctctaa gggcgaattc gcggccgcta
     7381 aattcaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     7441 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7501 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7561 tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     7621 tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc cccctggcca
     7681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     7741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     7801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     7861 tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc tagatccttt
     7921 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     7981 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     8041 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     8101 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     8161 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
     8221 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     8281 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     8341 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     8401 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8461 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     8521 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     8581 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8641 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8701 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     8761 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8821 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8881 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8941 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     9001 ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta
     9061 cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga
     9121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagattatca
     9181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9781 gtcatgccat ccgtaagatg cttttctgtg actggtgaat actcaaccaa gtcattctga
     9841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10141 caatattatt gaagcattta tcagggttat tgtctcatga ccaaaatccc ttaacgtgag
    10201 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10261 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10321 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10381 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10441 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10501 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10561 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10621 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    10681 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10741 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10801 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10861 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    10921 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    10981 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
