LOCUS       sequence-451922-       12407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative plasmid to express U2AF2-TurboID in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Gomes-Junior R, Moreira CMDN, Dallagiovanna B
  TITLE     Construction of a proximity labeling vector to identify 
            protein-protein interactions in human stem cells.
  JOURNAL   PLoS One. 2025 May 30;20(5):e0324779. doi: 
            10.1371/journal.pone.0324779. eCollection 2025.
  PUBMED    40445938
REFERENCE   2  (bases 1 to 12407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2025-05-30"; volume: "20(5):e0324779. doi"; pages: " 
            10.1371/journal.pone.0324779. eCollection 2025"
COMMENT     Sequence Label: AAVS1-TRE-U2AF2-TurboID-HA-rtTA
FEATURES             Location/Qualifiers
     source          1..12407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1105..1130
                     /label=SA
                     /note="splice acceptor site"
     CDS             1154..1207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1217..1816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1217..1236)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1713..1733
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1848..1865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1854..2078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2153..2171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2190..2208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2227..2245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2264..2282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2301..2319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2338..2356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2375..2393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2415..2439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4890..4916
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4943..5064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5225..5528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5802..6818
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6742..6764
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6826..6845
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(6880..7497,7498..7503,7504..7539,7540..7542,7543..7578,
                     7579..7581,7582..7617,7618..7626)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    7653..7774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7909..8745
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8779..8798)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8780..8798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8805..8822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8805..8821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8814..8836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8959..9261
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9140..9159
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             9610..10404
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9610..10404
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9664..9683)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10274..10293
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10654..11445,11446..11514))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11277..11296
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11638..12226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12127..12146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12380..12397
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
     1141 atctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2041 caatagcagg catgctgggg atgcggtggg ctctatgggt cgacgacgcg tccccgaaaa
     2101 gtgccacctg acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc
     2161 agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc
     2221 tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga
     2281 gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc
     2341 ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacc
     2401 ccctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2461 tgttttgacc tccatagaag acaccgggac cgatccagcc taccggtatg tcggacttcg
     2521 acgagttcga gcggcagctc aacgagaata aacaagagcg ggacaaggag aaccggcatc
     2581 ggaagcgcag ccacagccgc tctcggagcc gggaccgcaa acgccggagc cggagccgcg
     2641 accggcgcaa ccgggaccag cggagcgcct cccgggacag gcgacgacgc agcaaacctt
     2701 tgaccagagg cgctaaagag gagcacggtg gactgattcg ttccccccgc cacgagaaga
     2761 agaagaaggt ccgtaaatac tgggacgtgc cacccccagg ctttgagcac atcaccccaa
     2821 tgcagtacaa ggccatgcaa gctgcgggtc agattccagc cactgctctt ctccccacca
     2881 tgacccctga cggtctggct gtgaccccaa cgccggtgcc cgtggtcggg agccagatga
     2941 ccagacaagc ccggcgcctc tacgtgggca acatcccctt tggcatcact gaggaggcca
     3001 tgatggattt cttcaacgcc cagatgcgcc tgggggggct gacccaggcc cctggcaacc
     3061 cagtgttggc tgtgcagatt aaccaggaca agaattttgc ctttttggag ttccgctcag
     3121 tggacgagac tacccaggct atggcctttg atggcatcat cttccagggc cagtcactaa
     3181 agatccgcag gcctcacgac taccagccgc ttcctggcat gtcagagaac ccctccgtct
     3241 atgtgcctgg ggttgtgtcc actgtggtcc ccgactctgc ccacaagctg ttcatcgggg
     3301 gcttacccaa ctacctgaac gatgaccagg tcaaagagct gctgacatcc tttgggcccc
     3361 tcaaggcctt caacctggtc aaggacagtg ccacggggct ctccaagggc tacgccttct
     3421 gtgagtacgt ggacatcaac gtcacggatc aggccattgc ggggctgaac ggcatgcagc
     3481 tgggggataa gaagctgctg gtccagaggg cgagtgtggg agccaagaat gccacgctga
     3541 gcaccatcaa tcagacgcct gtgaccctgc aagtgccggg cttgatgagc tcccaggtgc
     3601 agatgggcgg ccacccgact gaggtcctgt gcctcatgaa catggtgctg cctgaggagc
     3661 tgctggacga cgaggagtat gaggagatcg tggaggatgt gcgggtcgag tgcagcaagt
     3721 acgggcttgt caagtccatc gagatccccc ggcctgtgga cggcgtcgag gtgcccggct
     3781 gcggaaagat ctttgtggag ttcacctctg tgtttgactg ccagaaagcc atgcagggcc
     3841 tgacgggccg caagttcgcc aacagagtgg ttgtcacaaa atactgtgac cccgactctt
     3901 atcaccgccg ggacttctgg ttcgaaaagg acaacacggt gcccctcaag ctcattgcac
     3961 tcctcgcaaa cggagagttc cacagcggag agcaactcgg agagacgctc ggaatgagcc
     4021 gcgcagcaat taacaagcac attcaaacgc tccgcgactg gggagtggac gtgttcacgg
     4081 tgcccggaaa gggatacagc ctccccgagc ccattcccct cctcaacgca aagcaaattc
     4141 tcggacaact cgacggagga agcgtggcag tgctccccgt ggtggacagc acgaaccaat
     4201 acctcctcga ccgcattgga gagctcaaga gcggagacgc atgcattgca gagtaccaac
     4261 aagcaggacg cggaagccgc ggacgcaagt ggttcagccc cttcggagca aacctctacc
     4321 tcagcatgtt ctggcgcctc aagcgcggac ccgcagcaat tggactcgga cccgtgattg
     4381 gaattgtgat ggcagaggca ctccgcaagc tcggagcaga caaggtgcgc gtgaagtggc
     4441 ccaacgacct ctacctccaa gaccgcaagc tcgcaggtat tctcgtggag ctcgcaggaa
     4501 ttacgggaga cgcagcacaa attgtgattg gagcaggaat taacgtggca atgcgccgcg
     4561 tggaggagag cgtggtgaac caaggatgga ttacgctcca agaggcagga attaacctcg
     4621 accgcaacac gctcgcagca acgctcattc gcgagctccg cgcagcactc gagctcttcg
     4681 agcaagaggg actcgcaccc tacctccccc gctgggagaa gctcgacaac ttcattaacc
     4741 gccccgtgaa gctcattatt ggagacaagg agattttcgg aattagccgc ggaattgaca
     4801 agcaaggagc actcctcctc gagcaagacg gagtgattaa gccctggatg ggaggagaga
     4861 ttagcctccg cagcgcagag aaggcaagct acccctacga cgtgcccgac tacgcataat
     4921 taattaattc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4981 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     5041 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     5101 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaggcgc
     5161 gccctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     5221 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     5281 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     5341 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     5401 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     5461 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     5521 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     5581 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     5641 gggggggggg gggcgcgccg gggggggggg gggggggggg gggggggggg ggggsgrggs
     5701 ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     5761 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     5821 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     5881 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     5941 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     6001 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     6061 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     6121 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     6181 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     6241 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     6301 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     6361 cgggcggggg gtggcggcag gkgggggtgc cgggcggggc ggggccgccy cgggccgggg
     6421 ggggckcggg ggrggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     6481 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     6541 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     6601 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     6661 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     6721 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     6781 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     6841 ttgtgctgtc tcatcatttt ggcaaagaat tccgccacca tgtctagact ggacaagagc
     6901 aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca
     6961 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     7021 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     7081 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc
     7141 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     7201 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     7261 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     7321 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     7381 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     7441 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     7501 ccggccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     7561 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     7621 gggtaagata tcgccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     7681 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7741 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7801 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     7861 tggtaaaatc gcgataagga tccgggctgg tcgacagtac taagctttac tagggacagg
     7921 attggtgaca gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt
     7981 ctaaccccca cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga
     8041 gccatctctc tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc
     8101 tgggagggag agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt
     8161 ctcagtggcc accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct
     8221 ggtgcgtttc actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt
     8281 ttggaaaaac aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc
     8341 tagtccccaa tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta
     8401 gccagccccg tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct
     8461 ttgtgagaat ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc
     8521 tttctccatc cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc
     8581 cagagcaggg tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc
     8641 aagcccagga gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc
     8701 ctggctctcc tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg
     8761 gccgctaaat tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     8821 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8881 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8941 cgcagcctat acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     9001 ctgtttgtgg atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc
     9061 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     9121 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     9181 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     9241 ctgatgttct ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag
     9301 atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
     9361 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
     9421 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
     9481 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
     9541 ttgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga
     9601 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     9661 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     9721 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     9781 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     9841 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     9901 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     9961 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
    10021 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
    10081 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
    10141 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
    10201 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
    10261 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
    10321 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
    10381 cgccttcttg acgagttctt ctgaattatt aacgcttaca atttcctgat gcggtatttt
    10441 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
    10501 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    10561 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10621 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10681 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10741 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10801 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10861 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10921 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10981 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11041 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11101 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11161 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgaatact caaccaagtc
    11221 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11281 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11341 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11401 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11461 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11521 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgacca aaatccctta
    11581 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    11641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    11701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    11761 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    11821 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    11881 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    11941 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    12001 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    12061 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    12121 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    12181 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    12241 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    12301 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    12361 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
