LOCUS       sequence-451923-        9179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic tool for blue-light inhibition and red-light excitation 
            of neurons fused to mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Adesnik H
  TITLE     Miscellaneous Optogenetic Tools
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-BiPOLES-mRuby3
FEATURES             Location/Qualifiers
     source          1..9179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          700..1716
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1640..1662
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1724..1743
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2740..3447
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     CDS             3808..4851
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     intron          5075..5207
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5190..5209
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5216..5804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5269..5289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5687..5698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5831..5848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5837..5948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6044..6064)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6044..6062)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6083..6099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6201..6220)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6266..6321
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6320..6339)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6682..6698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6706..6722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6730..6736,6737..6754,6755..6760))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6775..6796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6855..7050
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6901..7036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7056..7190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7093..7112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7147..7166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7258..7275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7429..8017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7509..7528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8188..8979,8980..9048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8811..8830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9049..9153)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggggg ggccccgggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg ggggggggcg gggggggggg gcggggcggg gcggagaggt
      601 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      661 cggcggcggc cctataaaag gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      721 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      781 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      841 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      901 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      961 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     1021 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     1081 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     1141 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     1201 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     1261 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     1321 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     1381 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     1441 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     1501 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1561 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1621 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1681 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1741 gtgctgtctc atcattttgg caaagaattc ggtaccggat ccatggcatc acaggtcgtc
     1801 tatggggaat gggcatcaac tcacaccgag tgctacaata tgtccagaat cgactccacc
     1861 ttcgtctcac tgctgcagct ggtgtgggcc gtggtctcag gctgccagac aatcttcatg
     1921 attagcaggg ctcccaaggt cccttgggag tccgtgtacc tgccctttgt cgaatctatc
     1981 acttatgcac tggccagtac tggcaacgga accctgcaga tgagggacgg cagattcttt
     2041 ccttggagcc gaatggcctc ctggctgtgc acctgtccaa tcatgctggg acagatttct
     2101 aacatggctc tggtgaagta caaaagtatc ccactgaatc ccattgcaca ggccgcttcc
     2161 atcattagag tggtcatggg aatcaccgct acaatttctc ccgcagagta tatgaagtgg
     2221 ctgttctttt tctttggggc cacctgcctg gtgttcgagt acagtgtggt cttcacaatc
     2281 tttcaagtgg ggctgtatgg ctttgaatct gtcgggacac ctctggcaca gaaggtggtg
     2341 gtgaggatca agatgctgcg cctgatcttc tttattgctt ggactatgtt cccaatcgtg
     2401 tggctgattt cacccaccgg agtctgcgtg atccacgaga acgtgagcgc cattctgtac
     2461 ctgctggctg acggcctgtg caaaaataca tatggagtga tcctgtggag cactgcctgg
     2521 ggagtgctgg aagggaagtg ggaccccgca tgtctgccag ggcaggagaa acccgaagcc
     2581 gacgatcctt ttggcctgaa tcacgagaag aatgcccctc ctaacgacga agtcaatatc
     2641 agaatgtttg gaaggggacg tacggcggcc aagagcagga tcaccagcga gggcgagtac
     2701 atccccctgg accagatcga catcaacgtg gcggtacccg tgtctaaggg cgaagagctg
     2761 atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc
     2821 aaatgcacag gtgaaggaga aggcagaccg tacgagggag tgcaaaccat gaggatcaaa
     2881 gtcatcgagg gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat
     2941 ggcagccgta cctttatcaa gtacccggcc gacatccctg atttctttaa acagtccttt
     3001 cctgagggtt ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc
     3061 acgcaggaca ccagccttga ggatggcgag ctcgtctaca acgtcaaggt cagaggggta
     3121 aactttccct ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca
     3181 gagatgatgt atccagcaga tggtggtctg agaggataca ctgacatcgc actgaaagtt
     3241 gatggtggtg gccatctgca ctgcaacttc gtgacaactt acaggtcaaa aaagaccgtc
     3301 gggaacatca agatgcccgg tgtccatgcc gttgatcacc gcctggaaag gatcgaggag
     3361 agtgacaatg aaacctacgt agtgcaaaga gaagtggcag ttgccaaata cagcaacctt
     3421 ggtggtggca tggacgagct gtacaagacc ggtgcagccc tgcaggagaa gaagtcatgc
     3481 agccagcgca tggccgaatt ccggcaatac tgttggaacc cggacactgg gcagatgctg
     3541 ggccgcaccc cagcccggtg ggtgtggatc agcctgtact atgcagcttt ctacgtggtc
     3601 atgactgggc tctttgcctt gtgcatctat gtgctgatgc agaccattga tccctacacc
     3661 cccgactacc aggaccagtt aaagtcaccg ggggtaacct tgagaccgga tgtgtatggg
     3721 gaaagagggc tgcagatttc ctacaacatc tctgaaaaca gctctagaca ggcccagatc
     3781 accggacgtc cggagggagc tgtacaagag ctgatcagca gcgccaccag atctctgttt
     3841 gccgccggag gcatcaaccc ttggcctaac ccctaccacc acgaggacat gggctgtgga
     3901 ggaatgacac ctacaggcga gtgcttcagc accgagtggt ggtgtgaccc ttcttacgga
     3961 ctgagcgacg ccggatacgg atattgcttc gtggaggcca caggcggcta cctggtcgtg
     4021 ggagtggaga agaagcaggc ttggctgcac agcagaggca caccaggaga aaagatcggc
     4081 gcccaggtct gccagtggat tgctttcagc atcgccatcg ccctgctgac attctacggc
     4141 ttcagcgctt ggaaggccac ttgcggttgg gaggaggtct acgtctgttg cgtcgaggtg
     4201 ctgttcgtga ccctggagat cttcaaggag ttcagcagcc ccgccacagt gtacctgtct
     4261 accggcaacc acgcctattg cctgcgctac ttcgagtggc tgctgtcttg ccccgtgatc
     4321 ctgatcaagc tgagcaacct gagcggcctg aagaacgact acagcaagcg gaccatgggc
     4381 ctgatcgtgt cttgcgtggg aatgatcgtg ttcggcatgg ccgcaggact ggctaccgat
     4441 tggctcaagt ggctgctgta tatcgtgtct tgcatctacg gcggctacat gtacttccag
     4501 gccgccaagt gctacgtgga agccaaccac agcgtgccta aaggccattg ccgcatggtc
     4561 gtgaagctga tggcctacgc ttacttcgcc tcttggggca gctacccaat cctctgggca
     4621 gtgggaccag aaggactgct gaagctgagc ccttacgcca acagcatcgg ccacagcatc
     4681 tgcgacatca tcgccaagga gttttggacc ttcctggccc accacctgag gatcaagatc
     4741 cacgagcaca tcctgatcca cggcgacatc cggaagacca ccaagatgga gatcggaggc
     4801 gaggaggtgg aagtggaaga gttcgtggag gaggaggacg aggacacagt gctcgagcag
     4861 agccagccca tcctgaacac caaggagatg gccccccaga gcaagccccc cgaggagctg
     4921 gagatgagca gcatgcccag ccccgtggcc cccctgcccg cccgcaccga gggcgtgatc
     4981 gacatgcgca gcatgagcag catcgacagc ttcatcagct gcgccaccga cttccccgag
     5041 gccacccgct tctgatctag actcgaggcc gcaggtaagt atcaaggtta caagacaggt
     5101 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
     5161 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tcgacaatca
     5221 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5281 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5341 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5401 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5461 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5521 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5581 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     5641 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5701 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5761 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg
     5821 gtacgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5881 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5941 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6001 agcaaggggg aggattggga agacaatagc ccagcttttg ttccctttag tgagggttaa
     6061 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctaa
     6121 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     6181 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     6241 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     6301 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     6361 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     6421 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     6481 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     6541 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     6601 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     6661 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6721 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6781 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6841 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7021 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     7081 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     7201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
