LOCUS       sequence-451925-       11711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative plasmid to express GFP-TurboID in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Gomes-Junior R, Moreira CMDN, Dallagiovanna B
  TITLE     Construction of a proximity labeling vector to identify 
            protein-protein interactions in human stem cells.
  JOURNAL   PLoS One. 2025 May 30;20(5):e0324779. doi: 
            10.1371/journal.pone.0324779. eCollection 2025.
  PUBMED    40445938
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2025-05-30"; volume: "20(5):e0324779. doi"; pages: " 
            10.1371/journal.pone.0324779. eCollection 2025"
COMMENT     Sequence Label: AAVS1-TRE-GFP-TurboID-HA-rtTA
FEATURES             Location/Qualifiers
     source          1..11711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1105..1130
                     /label=SA
                     /note="splice acceptor site"
     CDS             1154..1207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1217..1816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1217..1236)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1713..1733
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1848..1865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1854..2078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2153..2171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2190..2208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2227..2245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2264..2282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2301..2319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2338..2356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2375..2393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2415..2439
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2508..2510,2511..2513,2514..3224)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2508..2510,2511..2513,2514..3224)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2553..2574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2814..2833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3161..3182
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4194..4220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4247..4368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        4529..4832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5106..6122
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6046..6068
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6130..6149
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(6184..6801,6802..6807,6808..6843,6844..6846,6847..6882,
                     6883..6885,6886..6921,6922..6930)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    6957..7078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7213..8049
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8083..8102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8084..8102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8109..8126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8109..8125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8118..8140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8263..8565
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8444..8463
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8914..9708
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8914..9708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8968..8987)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9578..9597
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9958..10749,10750..10818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10581..10600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10942..11530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11431..11450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11684..11701
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
     1141 atctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2041 caatagcagg catgctgggg atgcggtggg ctctatgggt cgacgacgcg tccccgaaaa
     2101 gtgccacctg acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc
     2161 agtgatagag aaggtacgtc tagaacgtct ccctatcagt gatagagaag tcgacacgtc
     2221 tcgagctccc tatcagtgat agagaaggta cgtctagaac gtctccctat cagtgataga
     2281 gaagtcgaca cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc
     2341 ctatcagtga tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacc
     2401 ccctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2461 tgttttgacc tccatagaag acaccgggac cgatccagcc taccggtatg gtgagcaagg
     2521 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2581 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2641 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2701 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2761 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2821 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2881 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2941 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3001 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3061 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3121 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3181 tgaccgccgc cgggatcact ctcggtatgg acgagctgta caagttcgaa aaggacaaca
     3241 cggtgcccct caagctcatt gcactcctcg caaacggaga gttccacagc ggagagcaac
     3301 tcggagagac gctcggaatg agccgcgcag caattaacaa gcacattcaa acgctccgcg
     3361 actggggagt ggacgtgttc acggtgcccg gaaagggata cagcctcccc gagcccattc
     3421 ccctcctcaa cgcaaagcaa attctcggac aactcgacgg aggaagcgtg gcagtgctcc
     3481 ccgtggtgga cagcacgaac caatacctcc tcgaccgcat tggagagctc aagagcggag
     3541 acgcatgcat tgcagagtac caacaagcag gacgcggaag ccgcggacgc aagtggttca
     3601 gccccttcgg agcaaacctc tacctcagca tgttctggcg cctcaagcgc ggacccgcag
     3661 caattggact cggacccgtg attggaattg tgatggcaga ggcactccgc aagctcggag
     3721 cagacaaggt gcgcgtgaag tggcccaacg acctctacct ccaagaccgc aagctcgcag
     3781 gtattctcgt ggagctcgca ggaattacgg gagacgcagc acaaattgtg attggagcag
     3841 gaattaacgt ggcaatgcgc cgcgtggagg agagcgtggt gaaccaagga tggattacgc
     3901 tccaagaggc aggaattaac ctcgaccgca acacgctcgc agcaacgctc attcgcgagc
     3961 tccgcgcagc actcgagctc ttcgagcaag agggactcgc accctacctc ccccgctggg
     4021 agaagctcga caacttcatt aaccgccccg tgaagctcat tattggagac aaggagattt
     4081 tcggaattag ccgcggaatt gacaagcaag gagcactcct cctcgagcaa gacggagtga
     4141 ttaagccctg gatgggagga gagattagcc tccgcagcgc agagaaggca agctacccct
     4201 acgacgtgcc cgactacgca taattaatta attcgagcag acatgataag atacattgat
     4261 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4321 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4381 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     4441 aacctctaca aatgtggtag gcgcgcccta gttattaata gtaatcaatt acggggtcat
     4501 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     4561 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4621 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4681 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4741 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     4801 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
     4861 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
     4921 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccggggggg gggggggggg
     4981 gggggggggg gggsggggsg rggsggrgrg gkgcggcggc agccaatcag agcggcgcgc
     5041 tccgaaagtt tccttttatg gcgaggcggc ggcggcccta taaaaagcga agcgcgcggc
     5101 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     5161 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     5221 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     5281 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     5341 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     5401 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     5461 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     5521 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     5581 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     5641 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggkgggg gtgccgggcg
     5701 gggcggggcc gccycgggcc ggggggggck cgggggrggg gcgcggcggc ccccggagcg
     5761 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     5821 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     5881 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     5941 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     6001 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     6061 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     6121 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattccgcc
     6181 accatgtcta gactggacaa gagcaaagtc ataaacggcg ctctggaatt actcaatgga
     6241 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     6301 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     6361 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     6421 cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     6481 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     6541 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     6601 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     6661 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     6721 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     6781 cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg
     6841 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     6901 gattttgacc ttgacatgct ccccgggtaa gatatcgccg cttcgagcag acatgataag
     6961 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7021 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     7081 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     7141 aagcaagtaa aacctctaca aatgtggtaa aatcgcgata aggatccggg ctggtcgaca
     7201 gtactaagct ttactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc
     7261 cttcctagtc tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct
     7321 ggtgacacac ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc
     7381 ttacgatgga gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg
     7441 gggggatgcg tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc
     7501 tgagctgctc tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt
     7561 acacttccca agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt
     7621 tctaactttg gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt
     7681 tttacctgtg agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg
     7741 gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc
     7801 gtggccgcct ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg
     7861 ctatctggga catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg
     7921 ggcttctggg tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc
     7981 ttcctcgtcc accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg
     8041 ctctgctcta agggcgaatt cgcggccgct aaattcaatt cgccctatag tgagtcgtat
     8101 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8161 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8221 accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa ggtttacacc
     8281 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     8341 ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     8401 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     8461 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     8521 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggcatgaga
     8581 ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg
     8641 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     8701 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     8761 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     8821 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc
     8881 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     8941 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     9001 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     9061 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     9121 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     9181 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     9241 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     9301 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     9361 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     9421 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     9481 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     9541 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     9601 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     9661 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tattaacgct
     9721 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc
     9781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9841 ttcaaatatg tatccgctca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10501 gactggtgaa tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10861 ttgtctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    11101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    11281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    11341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    11401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    11461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    11521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    11581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11641 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11701 ggaagcggaa g
//
