LOCUS       sequence-45193-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-B5
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             314..337
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    344..377
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggaccatcct ctagactgcc ggatctagct agttaattaa ggatccgtta acaagcttga
       61 gggtttttaa tcgtctttta gtttctgagt attgtaagtt atgttagact gtaattatga
      121 aatgaactgt tttgctgtta tcaggtggaa cacgatgcaa ttttggttgg tgtaatagga
      181 ggaaaattgc acggtatcca tctgtaaacc gcaggaccta tgtggacagc cgtctatcag
      241 tgctatagta agattgtaaa atcctttagc tagtcattgc tttgaacaca tttgggaatt
      301 cgtatacact agtgactaca aggacgacga tgacaagtga taaataactt cgtataatgt
      361 atgctatacg aagttattct agtatttaaa tgccctcgag ctgactgcgg ccgccagcac
      421 agtggtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
      481 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
      541 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
      601 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
      661 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      721 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
      781 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcct
//
