LOCUS       sequence-451931-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The 3rd generation of rabies vector expressing Flpo recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou 
            Y, Feng G, Wickersham IR
  TITLE     Third-generation rabies viral vectors allow nontoxic retrograde 
            targeting of projection neurons with greatly increased efficiency.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 
            10.1016/j.crmeth.2023.100644.
  PUBMED    37989085
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100644. doi"; pages: "
            10.1016/j.crmeth.2023.100644"
COMMENT     Sequence Label: pRV∆L-5Flpo
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(985..1004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(986..1004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1049..1252)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1078..1098)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1253..1632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1674..1690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1683..1705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(1831..2287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1969..1990)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2181..2200
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2314..2418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2419..2487,2488..3279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2637..2656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3450..4038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3939..3958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4192..4209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4326..4347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4362..4367,4368..4385,4386..4392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4400..4416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4405..4427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4424..4440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4424..4440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4459..4479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4461..4479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4516..4532
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4516..4532
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(4555..4602)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4598..4616
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(join(4896..6167,6168..6188,6189..6194))
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     regulatory      6191..6200
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaggaaca gctgtctcct gccctggctc aaacattctt cttatctctt cctcaaagtt
       61 cttgtggaag aaatatagta ttgcctctct agcggtgaga ttgatttgtt ttatgaaccc
      121 agtaaatgat accagtcctg aacagtcttc ataagcagtg acaactgtgc ccactctgat
      181 tgctgaatat agatgctcaa tccgggagaa aaacatgtca taggttccgg ccaaaaatct
      241 gaagtttggt atagtactcc aattagcaca cattttgtga gttgtcatta gagtatggtg
      301 ttccacgatt ttaacaaaag gggctgtctc aaaaatctgc tctatcctgt ctgcaatgtt
      361 tgtcttatag ttaccagtgt tttgcccgga tattttgctc aacctataca gactcaagag
      421 aagaccgact aaggacgcat gctcagggac agtggggtct cttgtcagtt ccatgcctcc
      481 tgtcagagcc caattccctt ctacatcagt acgttttatc tccaccagag aacctggggt
      541 gatcttatct ccttttcgtg caatcacaat tccatagctg gtccagtctt ccggacatgt
      601 cccctcaaaa aactgcattg ccgctgccaa ataggaacat acatcgtcag gattaagttt
      661 ggcggcgctc atgcctgaca aaactgactt gtatgcttta tttaaatcgg gagcctttcc
      721 tagggttata cagggctttt tcaaatcttt gatggcaggg tacttgtact catattgatc
      781 cacgataatc tcaggcttca aagagaccac ctgattattg actttgaata caatcttgtc
      841 ggcatccatt gtaggggtgt tacatttttg ctttgcaatt gacaatgtct gttttttctt
      901 tgatctggtt gttaagcgtg acccggtacg ccgggtttcg tcctcacgga ctcatcagac
      961 gcttaatcta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
     1021 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
     1081 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     1141 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     1201 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     1261 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     1321 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     1381 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     1441 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     1501 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     1561 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     1621 ataatcaatg tcaacgcgct gcaggaattc gatatcaagc ttatcgcgcg ctcactggcc
     1681 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1741 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1801 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
     1861 tcgcgttaaa tttttgttaa atcagctcat ttttttaacc aataggccga aatcggcaaa
     1921 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1981 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2041 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2101 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2161 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2221 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2281 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2341 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2401 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2461 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2521 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2581 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2641 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2701 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2761 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2821 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2881 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2941 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3001 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3061 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3121 gcctccaagg gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3181 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3241 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3301 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3361 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3421 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3481 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3541 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3601 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3661 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3721 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3781 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3841 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3901 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3961 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4021 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4081 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4141 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4201 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4261 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4321 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4381 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4441 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
     4501 cgcggtggcg gccgctctag aactagtgga tccggatata gttcctcctt tcagcaaaaa
     4561 acccctcaag acccgtttag aggccccaag gggttatgct agttattgct cagcggtggc
     4621 agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatccccgc cctcccttag
     4681 ccatccgagt ggacgtgcgt cctccttcgg atgcccaggt cggaccgcga ggaggtggag
     4741 atgccatgcc gacccacgct taacaaataa acaacaaaaa tgagaaaaac aatcaaacaa
     4801 ccaaaggttc agatttagga tcttgttttt ttcaagatac atcacacaag agtcttagca
     4861 tgggcaggct tccaggagta tccggtcgta cgtcatcaga tccgcctgtt gatgtagctg
     4921 ctcaggtagt ccagcacctc ctggctgatg atgccgttcc aggcggggta tctgatgctg
     4981 ccctcggcgc tgcccttcag ctgctcgatg tgctgccact cctcgatggg gttggtctcg
     5041 tccttcaggg cgatcatctc cttgctgatg gggtcgtagg cgtagtacct ggacaccagg
     5101 gcgaagtagt ggtcggggat ggcggtgatc tggtgggtgt aggtggtcct ggccacggcg
     5161 gaggccctct tgtcgctcca gttgcccacc acgtttgtca gctcggtcag gcccttcatg
     5221 ctcagaaagc tggtcatcag gtgcctgccg atgtggctct tagggccgtt cttgatagcg
     5281 aagatggggt agggggcgtt cttcttcagg gccttgttgt agctgcgcac caggttgtcc
     5341 ttcagcagct ggtactcctg cttgttgctg ctgctgttgc cggtcctgtt cactctcttc
     5401 agcacgggct cgctgttcct caggaactcg tccaggtaca ccagggggtc gatcctgcct
     5461 ctggcgctga aaaagtagat gtgcctggac acgcttgtct tggtctcggt caccaggcac
     5521 tgaatgatca cgcccaggta cttgttctgc accagcttga agctcttggg gtccacgttc
     5581 ttgatgtcgc tgaacctgcc gcagttgatg aatgtggcca ggaacaggaa ctggtacagg
     5641 gtcttggtct tggtgaacct gctggtgtac tcgaagctgt tcaggatctt ctcggtgatc
     5701 tcccagatgc tctcgccctc ggacagcagg gccttcagca tcttcttgct gtggctgttg
     5761 cccttgtcgg cctcctcgct gctctcgaac tgcagctgca ggctggacac gatgtcggtg
     5821 atgtcgctct ggtgcttctg gccgttgtaa gggatgatgg tgaactccca ggcggggatc
     5881 agcttcttca ggctggcctc caggatggtg gccttctggg tcttgtactt gaactgcagg
     5941 ctcttgttca cgatgtcgaa gctcaggctg ttgctgatga tggtgttgta gctcatgaag
     6001 gtggccctct tgatggcggt gccgttgtgg gtgatcatcc agcacaggta ggtcagctcg
     6061 gcggcacagc tggcgatctt ctcgccgctg ggcctctcga atctctccac gaactgccgc
     6121 accagcacct tggggggggt cttgcacagg atgtcgaact ggctcatcac cttcctcttc
     6181 ttcttaggag ccatggtggc ggctttgcta gcggaggggt gttagttttt ttcatggact
     6241 tggatcgttg aaaggacggc cagtccggta ccttacagtc tggtctcacc cccactcttg
     6301 tgtgattccc atgaagatat gatcttcccg ctttggggag tgactgacac ctccctccct
     6361 gtccctctga gattgtgttg cgtaggttct gatcgattga ctcttctaca acatgtcatc
     6421 aggaaaatta tcaacatcaa ggcagtcagg gcccctgcac tcagtaatac atacttcccc
     6481 cagttcggga gacccaagtc aactcctgag acctgattgt gcacatcggg aaggtgaact
     6541 tcaacaaaat cctcagcctc gtcaccgtcc ttgaaaacgg tagacgggtc tgccaggggg
     6601 tgcacaaggg ggataaccga ggattccaac aactccatat gttgctggag gagggatgat
     6661 tgcatctctg ggattaagac attgccgtca ggtcctaata ttataccatt gaaaaacacc
     6721 ccgttcacat gaggatgaca cctcccccca actcttaaac acccttttga agggaggatc
     6781 tcattccaag ttctgactga cttgtagtga gcatcggctt ccatcaaggt cttgttgaat
     6841 atggtatatg cttttccaaa cccagggaca agttttctta aatgactgag acgtctgaaa
     6901 ctcactgact tggttgtcat gatggactct agtgcatcca gacactcctc tctcttcctg
     6961 accaactcct ctacaacaag gtgctcaatt tcgtctgagc gaaagtcgtg caggttcacc
     7021 aacttatcgg gagggcacca tttggtttca tttgatgttt gcatcgagac ccatgttcca
     7081 tccataagtc taagtcctag aactccacat aacttgagtt tgcatgctcc ttttaaagac
     7141 ttatataggc ctctttcatc tacaaagccg caagtctcac tccctttgga tgctctcttc
     7201 cctctactat tggtaaaaat gtcacaagac atccctagtc tcggattctc gggcatccaa
     7261 atggtgtaat cgtggttagt ggagcagtag gtagaagaca ccgctactcc tgagcacttc
     7321 ccgctaggga agaccctcga gtgaagggat ctgtcatatg ggtccaaatc tgccacactt
     7381 ggagatatga taacgagaga ctccttggtg gtttttacag ttcgaagcca gcggtagtca
     7441 gggtacggat tgtgtagaga ctcttcatat ctggggtcac cggccatctt ccagttgtac
     7501 gcggctctac atgcatctgg tgttgggcgg aaatgctttc ttttgaacgt ggttgtgaca
     7561 taaccaacga agttagtgta ggtttcagcc tccgtcacaa cgcctgtgca agtgaacccg
     7621 ttcactttta tggctaagat gtatccaact ttaagttcca tgtaggagaa ccctgacagg
     7681 ttggtgcatc cttcgtcctc cactaccaaa ttgtttgggc agctgaggtg atgtatgtca
     7741 atcggactcc agggaccaag cttgtctggt atcgtgtaaa tagggaattt cccaaaacac
     7801 aatggaaaaa ccagaagggg tacaaacagg agagcctgag gaaccatctt tccttggcgc
     7861 gcctcttttg agggatgtta atagtttttt tcacatccaa gaggatcaaa atgtaaaggt
     7921 aatcaacttg actttgatga aatgcagcgg tttaatggca ccataacatg ttttttgtta
     7981 tattgctccc aagggttgga gttgagccca tatgttctct cctccagagg taaacaagtg
     8041 ataaatttgc gggatataat ctgattattc tagaagcaga gaggaatctt tgtcctcttc
     8101 ggacctttgt gtctgaagag acatgtcaga ccatagttga catgctctcg ggttcatgtt
     8161 gatacaccag actctgccct ggatatgaca ctgttttgca atcactctta tttgcaatcc
     8221 gacgaactca gtatcatcat cccaagtgat ctcctgagag tattccaact cctccccttc
     8281 aagagggccc ctggaatcag cccactggaa gataaaggtt ctcctcaatt tgtataccca
     8341 gttcaggccc tcagggactg gagatcctga caaagccagt ccaataacca ctttgactaa
     8401 cccgatcatc ctatgattcc cagaatatat ctcgtcgaat gatttcagaa tgtgccgcag
     8461 gatcctgaac gagtaaccat tcgggctaca cactttaacc cttccgttga tacaaaagtt
     8521 cctcatgttc ttcttgcctg taagttcttt cagcgggacg tattcagggg gtggaagcca
     8581 caagtcatcg tcatccagag gggctgacgc gggagaggat ttttgagtgt cctcgtccct
     8641 gcggtttttc actatcttac gtaggaggtt cattttatca gtggtgttgc ctgttttttt
     8701 catgttgact ttgggacatc tcggatttta ttgtctagag ggactgaggg gagaggttcg
     8761 gttagcaaga tgtatagcga ttcaagtcat cttgcatgat tttactcagc ttgtcggatt
     8821 cgactaacaa ctggaatttc ttagagttgg ccaaagcgac ccatcccagt acacatctta
     8881 gggggagttt ggacccgtca tgggctaaac gggtcacacc tggtacattt tttgcctctt
     8941 taactatatc atcaaggttc attttcaatt gctcaaaatt atacaagagt atccctgagg
     9001 atcgagaggg aaacttatat tttttggaga aactttctgc aatctggtga gcgatctcag
     9061 cctccactga tagatcatcc tcttcattgg tagccgacca ttcaagggct ggagggccag
     9121 aagcaatttg agccgccatc ctggctttcg atgattggct ttctctctga gaaggagtgg
     9181 gtgttgtctc cttcttgagc tctcggccag tagtctgggt tgatttatcc tctgaagact
     9241 ttcctggagg gttgggaaag ttgaccgcga cataggatat aatctcttct acggtctgtg
     9301 accatatctt gagaaatctc tctcctgacc tcatttgtct gactatttgg actccaacat
     9361 tttccaggta tgactggaac aggaagctag gatcctctcc ttcatccatc tgaaagtcct
     9421 ctcgatactt gccttctccc accttggcta tctcaccatg gttgggcgat tttccatcat
     9481 ccaggtgaag tcgccccata tcctcaggga gattgtccac ctctatgggt tccccttgga
     9541 gatgagcctg attgtcttcg atatttctat tgatcagatc aacagtttct tcagccatct
     9601 caagatcggc cagaccggct ctaatagcac taggattgac aaagatcttg ctcatgtttg
     9661 ggatggttcg aaaggagggg tgttagtttt tttcatgatg gatatacaca atccgtagat
     9721 ttccggcatt ttgttattca acttcttatg agtcactcga atatgtcttg tttagaaact
     9781 cggcgaatga gtttggacgg gcttgatgat tggaactgac tgagacatat ctccgtatgt
     9841 gagatctctt tagtcgacct ccattcatca tgattcgagt ataaacagcc tccggacttc
     9901 tggtttcacc tgaaaagtag tcctcgtcgt cagagttgac agttccatca tctgccagtg
     9961 ctacgtcagt ctttgtcagt tcagccgcct cgtattcttg aagttctttc tcatctctga
    10021 agaatcttct ttcaaatgtc cctttcccga agaattcctc tcccagatag ccccctagaa
    10081 cagacatttc atgaggagca catgcagcaa taaccgttgc atttagggat ctgacttgac
    10141 ccatatagca tcctacaaag tgaatgagat tgaacacgtg accaacagca tttgatgaat
    10201 aaggagattt cccactcaag cctagtgaac ggaagtggat gaaataaga
//
