LOCUS       sequence-451935-        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxGold
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1078..1095
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctcag gaacagacga agaaatagca gaattcgtaa aagcattcta cgaagcactc
       61 gacgcaggag acgcagaaac agcagcagac ctactattcg gagcaggatg caaagaaata
      121 cacctatggg acggaacagt attcacaagc aaagaaggat tcgaaacatg gttcaaaaaa
      181 ctacaaagcg cagtaacagg agcaaaaaga aaaatagaaa gcgtagaaat aaaagacgga
      241 gaagcagtag taaaactaac actaacagca acatacaaag caacaggaaa aaaattcgta
      301 gtagaactag aacacacatt cacattcgac agagaaagaa acgaactagt agaagtgaaa
      361 gtcacagcaa caccatattt taccggcgtg gtgccgattc tggttgaatt ggatggtgat
      421 gtgaacggcc ataaattttc tgtgagcggt gaaggggaag gcgacgcgac ctatggtaaa
      481 ctgaccttga aatttatttg caccaccgga aaactgccag tgccgtggcc gaccctggtg
      541 actagcttgg gttatggtct gcagtgcttt gcgcgctatc cggaccacat gaaacagcat
      601 gatttcttta aaagcgctat gccggaaggc tatgtgcagg aacgcaccat tttcttcaaa
      661 gatgatggta actataaaac ccgcgccgaa gtaaaatttg aaggtgatac cttagtgaac
      721 cgcattgaac tgaaaggcat tgattttaaa gaagatggaa acattctggg ccacaaatta
      781 gaatataact acaacagcca taatgtgtat attaccgccg ataaacagaa aaatggcatt
      841 aaagccaatt ttaaaatccg ccataacatt gaagacggcg gcgtgcagct ggccgatcat
      901 tatcagcaga acaccccgat tggcgatggc ccggtgctgc tgccggataa ccattatctg
      961 agctatcaga gcaaactgag taaagatccg aacgaaaaac gcgatcacat ggtgttactg
     1021 gagtttgtga ccgcggcggg cattaccctg ggcatggatg aactgtataa aggttctcat
     1081 catcatcatc atcattaa
//
