LOCUS       sequence-451937-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxKOk
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1024..1041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctcag gaacagacga agaaatagca gaattcgtaa aagcattcta cgaagcactc
       61 gacgcaggag acgcagaaac agcagcagac ctactattcg gagcaggatg caaagaaata
      121 cacctatggg acggaacagt attcacaagc aaagaaggat tcgaaacatg gttcaaaaaa
      181 ctacaaagcg cagtaacagg agcaaaaaga aaaatagaaa gcgtagaaat aaaagacgga
      241 gaagcagtag taaaactaac actaacagca acatacaaag caacaggaaa aaaattcgta
      301 gtagaactag aacacacatt cacattcgac agagaaagaa acgaactagt agaagtgaaa
      361 gtcacagcaa caccaattct gccggaaatg aaaatgcgct attatatgga tggtagcgtg
      421 aacggccatg agtttaccat tgaaggcgaa ggcaccggcc gcccgtatga aggccatcaa
      481 gaaatgaccc tgcgtgtgac gatggcggaa ggcggcccaa tgccatttgc gtttgatctg
      541 gtgagccatg tgttttgcta tggccatcgc gtgtttacga aatatccgga agagattccg
      601 gattatttta aacaggcgtt tcctgaaggt ctgagctggg aaagaagcct ggagttcgaa
      661 gatggtggca gcgcgagcgt gagcgcacat attagcctgc ggggtaatac cttctatcat
      721 aaaagcaaat ttaccggcgt taactttccg gcggatggtc cgattatgca gaaccagagc
      781 gttgattggg aaccgagcac ggagaaaatt accgccagcg atggtgtgct gaaaggtgat
      841 gtgaccatgt atctgaaact ggagggcggc ggcaaccata aatgccaatt taaaaccacg
      901 tacaaagccg cgaaagaaat cttagaaatg ccgggcgacc attatattgg ccatagactg
      961 gttcgcaaaa ccgaaggtaa cattaccgaa caggttgaag atgcggtggc gcatagcggt
     1021 tctcatcatc atcatcatca ttaa
//
