LOCUS       sequence-451939-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxOFP
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1063..1080
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctcag gaacagacga agaaatagca gaattcgtaa aagcattcta cgaagcactc
       61 gacgcaggag acgcagaaac agcagcagac ctactattcg gagcaggatg caaagaaata
      121 cacctatggg acggaacagt attcacaagc aaagaaggat tcgaaacatg gttcaaaaaa
      181 ctacaaagcg cagtaacagg agcaaaaaga aaaatagaaa gcgtagaaat aaaagacgga
      241 gaagcagtag taaaactaac actaacagca acatacaaag caacaggaaa aaaattcgta
      301 gtagaactag aacacacatt cacattcgac agagaaagaa acgaactagt agaagtgaaa
      361 gtcacagcaa caccgtatat taaagaaaac atgcgtagca aactgtatct ggagggctct
      421 gtgaacggtc atcagtttaa atgcacccat gaaggtgaag gcaaaccgta tgaagggaaa
      481 cagactaacc gcattaaagt ggttgaaggc ggtccgctgc cgtttgcgtt tgatattctg
      541 gcgacccatt ttatgtatgg cagcaaagtg tttattaaat atccggcgga tctgccggat
      601 tattttaaac agtcgtttcc ggaaggtttt acctgggaac gcgtgatggt gtttgaagat
      661 ggtggcgtgc tgacggcgac tcaggatacc agcctgcagg atggtgaact gatttataac
      721 gtcaaagtgc gcggggttaa ctttccagcg aacggcccgg tgatgcagaa aaagacctta
      781 ggttgggaac cgagcaccga aaccatgtat ccagcggatg gcggtctgga aggccgctgc
      841 gataaagccc tgaaattagt gggcggcggc catctgcatg tgaactttaa aaccacgtac
      901 aaaagcaaaa agccggtgaa aatgccgggc gtgcattacg tggatcgccg tttagaacgc
      961 ataaaagaag cggataacga aacctatgtt gaacagtatg aacatgcagt ggcgcgctat
     1021 agcaacttag ggggcggcat ggatgaactg tacaaaggtt ctcatcatca tcatcatcat
     1081 taa
//
