LOCUS       sequence-451941-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxKate
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1066..1083
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctcag gaacagacga agaaatagca gaattcgtaa aagcattcta cgaagcactc
       61 gacgcaggag acgcagaaac agcagcagac ctactattcg gagcaggatg caaagaaata
      121 cacctatggg acggaacagt attcacaagc aaagaaggat tcgaaacatg gttcaaaaaa
      181 ctacaaagcg cagtaacagg agcaaaaaga aaaatagaaa gcgtagaaat aaaagacgga
      241 gaagcagtag taaaactaac actaacagca acatacaaag caacaggaaa aaaattcgta
      301 gtagaactag aacacacatt cacattcgac agagaaagaa acgaactagt agaagtgaaa
      361 gtcacagcaa caccatatat taaagaaaat atgcacatga aactgtatat ggaaggcacg
      421 gtgaacaacc accattttaa atgtaccagc gaaggtgaag gcaaaccgta tgaaggtacg
      481 cagaccatgc gcattaaagc ggttgaaggc ggtccgctgc cgtttgcgtt tgatattctg
      541 gcgaccagct ttatgtatgg cagcaaaacc tttattaacc atacccaggg cattccggat
      601 ttctttaaac agagctttcc ggaaggtttt acctgggaac gcgtgaccac gtacgaagat
      661 ggtggcgtgc tgactgcgac tcaagatacc agcctgcagg atggctgcct gatttataac
      721 gtgaaaattc gcggtgtgaa ctttccgagc aacggcccgg tgatgcagaa aaagacctta
      781 ggctgggaag cgagcaccga aaccctgtat ccggcggatg gcggtttaga aggccgtgcg
      841 gatatggcgc tgaaattagt gggcggcggc catctgattt gcaacctgaa aaccacttat
      901 cgttcgaaaa agccggcgaa aaacttaaaa atgccgggcg tgtattatgt ggatcgccgt
      961 ctggaacgca taaaagaagc ggataaagaa acctatgtcg aacagcatga agtggcggtg
     1021 gcgcgctatt gcgatctgcc aagcaaactg ggccatcgcg gttctcatca tcatcatcat
     1081 cattaa
//
