LOCUS       sequence-451953-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for de novo luciferase, neoLux1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pET29-neoLux1.2
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             382..399
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtcaggaa cagacgaaga aatagcagaa ttcgtaaaag cattctacga agcactcgac
       61 gcaggagacg cagaaacagc agcagaccta ctattcggag caggatgcaa agaaatacac
      121 ctatgggacg gaacagtatt cacaagcaaa gaaggattcg aaacatggtt caaaaaacta
      181 caaagcgcag taacaggagc aaaaagaaaa atagaaagcg tagaaataaa agacggagaa
      241 gcagtagtaa aactaacact aacagcaaca tacaaagcaa caggaaaaaa attcgtagta
      301 gaactagaac acacattcac attcgacaga gaaagaaacg aactagtaga agtgaaagtc
      361 acagcaacac cactaggttc ccaccaccac caccaccact aa
//
