LOCUS       sequence-45198-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pET28a-SpyTagMBP
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..106
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             116..154
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 tgcgaattcc ctctgaatat tttgtttaac tttaagaagg agatatacca tgggcagcag
       61 ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgggagccca
      121 catcgtgatg gtggacgcct acaagccgac gaagggtagt ggtgaaagtg gtaaaatcga
      181 agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt
      241 cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact
      301 ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc
      361 acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa
      421 agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct
      481 gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc
      541 gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg
      601 taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc
      661 tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt
      721 ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca
      781 catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc
      841 gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaatatgtg
      901 tacgtctgcg actcaggtca ctctacgt
//
