LOCUS       sequence-45205-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Mediouni S, Jablonski J, Paris JJ, Clementz MA, Thenin-Houssier S, 
            McLaughlin JP, Valente ST
  TITLE     Didehydro-cortistatin A inhibits HIV-1 Tat mediated 
            neuroinflammation and prevents potentiation of cocaine reward in Tat
            transgenic mice.
  JOURNAL   Curr HIV Res. 2015;13(1):64-79.
  PUBMED    25613133
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr HIV 
            Res."; date: "2015"; volume: "13(1)"; pages: "64-79"
COMMENT     Sequence Label: MDH1-PGK-FLAG-TAT-86-D
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..106
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(503..593)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(563..582)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tctctcttct catctccggg cctttcgacc tggcaccatg gactacaaag accatgacgg
       61 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa
      121 ttcagatcca gtagatccta acctagagcc ctggaaccat ccaggaagtc agcctaggac
      181 tccttgtaac aagtgtcatt gtaaaaagtg ttgctatcat tgcccagttt gcttcttaaa
      241 caaaggctta ggcatctcct atggcaggaa gaagcggaga cagcgacgag gacctcctca
      301 aggcggtcag gctcatcaag ttcctatacc aaagcaaccc tcctcccagc cccgagggga
      361 cccgacaggc ccgaaggaac agtaggtcga cctgcagcca agcttatcga taaaataaaa
      421 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
      481 gaattcgttt aaacgggctc gagtggtctc atacagaact tataagattc ccaaatccaa
      541 agacatttca cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgggatc
      601 cgctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
      661 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
      721 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
      781 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaatgct
      841 ggtatgatac act
//
