LOCUS       sequence-45209-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Mediouni S, Jablonski J, Paris JJ, Clementz MA, Thenin-Houssier S, 
            McLaughlin JP, Valente ST
  TITLE     Didehydro-cortistatin A inhibits HIV-1 Tat mediated 
            neuroinflammation and prevents potentiation of cocaine reward in Tat
            transgenic mice.
  JOURNAL   Curr HIV Res. 2015;13(1):64-79.
  PUBMED    25613133
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr HIV 
            Res."; date: "2015"; volume: "13(1)"; pages: "64-79"
COMMENT     Sequence Label: MDH1-PGK-FLAG-TAT-86-E
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..93
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(490..580)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(550..569)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tctccgggcc tttcgacctg caccatggac tacaaagacc atgacggtga ttataaagat
       61 catgacatcg attacaagga tgacgatgac aagcttgcgg ccgcgaattc agagccggta
      121 gatcctaacc tagagccctg gaatcatccg ggaagtcagc ctacaactgc ttgtagcaag
      181 tgttactgta aaaaatgttg ctggcattgc caactatgct ttctgaaaaa aggcttaggc
      241 atctcacacg gcaggaagaa gcggaagcac cgacgaggaa ctcctcagag cagtaaggat
      301 catcaaactc ctataccaga gcaacccctt cccatcatca gagggaaccc gacagacccg
      361 aaagaacagt aggtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt
      421 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagaa ttcgtttaaa
      481 cgggctcgag tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
      541 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgggatccgc tagcttaagt
      601 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
      661 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
      721 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
      781 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
      841 ttgaactaac caatcagttc
//
