LOCUS       sequence-45212-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..62
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     44..61
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    198..332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(222..241)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     276..295
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(445..465)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(445..463)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(484..500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(484..500)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(497..519)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(532..538,539..556,557..562))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    577..598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(715..732)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata
       61 gaatacaagc tacttgttct ttttgcagga tccactagtg gcgcgccatt aattaaaggc
      121 cggccagcga tcgccggaca tcgattggaa ttcaaggcct ctcgagcctc tagaactata
      181 gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca
      241 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      301 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      361 ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt
      421 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      541 agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      601 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      661 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatg
//
