LOCUS       sequence-45213-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Ohba T, Schirmer EC, Nishimoto T, Gerace L
  TITLE     Energy- and temperature-dependent transport of integral proteins to 
            the inner nuclear membrane via the nuclear pore.
  JOURNAL   J Cell Biol. 2004 Dec 20;167(6):1051-62.
  PUBMED    15611332
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2004-12-20"; volume: "167(6)"; pages: "1051-62"
COMMENT     Sequence Label: Reporter
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(226..492)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     promoter        complement(597..800)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(626..646)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgatgga tctgttggat attttagtat cttgaagaaa
       61 attagtgaag gggtttcctt ggttggtttc catagcttga tagaccaaaa acaaaaaacc
      121 cgccacgaga gcaaacagca gcatttttat ccacatggga acggagcgtc ccttctttgt
      181 cttttccgac ttgacggatc tgagctcgcc accgccaccc tcgacctgct ttgagattcg
      241 tcggaacaca tgataataga ggtcccaggc ttgggtgagg tccttgacat tccctgattt
      301 catgtacttc ctgcaccact cttgggcctc cattaaatct cgaccatagg cctgattaaa
      361 ggatgtttcc ttcagagtct gggggccccg ttccatcata gcatgcaagg gctccagcac
      421 ctcaaacatg cctttcacgt tcctttcccc aaagtacaaa cgagatgcct cttccaggcc
      481 ttcatgccac atggatctgg atctggatct ccttctatgc atagtctgca gaattcgaag
      541 cttgagctcg agatctgagt ccggtagcgc tagcggatct gacggttcac taaaccagct
      601 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      661 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      721 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      781 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccag
      841 t
//
