LOCUS       sequence-45214-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmCherry
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..61
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     43..60
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             147..857
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(292..310)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     560..579
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt gacactatag
       61 aatacaagct acttgttctt tttgcaggat ccactagtgg cgcgccatta attaaaggcc
      121 ggccagcgat cgccggacat cgattgatgg tgagcaaggg cgaggaggat aacatggcca
      181 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      241 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      301 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      361 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      421 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      481 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      541 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      601 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      661 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      721 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      781 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      841 acgagctgta caagtaagaa ttcaaggcct
//
