LOCUS       sequence-45215-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Mousseau G, Clementz MA, Bakeman WN, Nagarsheth N, Cameron M, Shi J,
            Baran P, Fromentin R, Chomont N, Valente ST
  TITLE     An Analog of the Natural Steroidal Alkaloid Cortistatin A Potently 
            Suppresses Tat-Dependent HIV Transcription.
  JOURNAL   Cell Host Microbe. 2012 Jul 19;12(1):97-108.
  PUBMED    22817991
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2012-07-19"; volume: "12(1)"; pages: "97-108"
COMMENT     Sequence Label: pcDNA3.1-TAT-2-84-FLAG
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(93..116)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(397..416)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(398..416)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(461..664)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(490..510)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtgagatga ccggtacgcg tagaatcgag accgaggaga gggttaggga taggcntttt
       61 attcgaaccg cgggccctct agactgcgtc tacttgtcat cgtcgtcctt gtagtccctt
      121 cttcgtctgc cctttcgttc ataacatatc ccgagcccct tgtttagaaa acacatctga
      181 caatggtagc agcatcgctt acaatagcat gagttattgc atgtttctag gggtcggtat
      241 agctgagaga ggatttcctc cccttgtctg gccaattctt gagtggccac atcctgctct
      301 gaagtgcgtg aaaagggctc gttgcaggac tttaatgagc tctctggcgc cttcaagggt
      361 gtctccatgg taagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      421 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      481 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      541 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      601 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      661 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
      721 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
      781 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
      841 ccaccccatt gacgtcaatg gaaagtccct atttggcgtt actatgggaa c
//
