LOCUS       sequence-45218-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Mousseau G, Clementz MA, Bakeman WN, Nagarsheth N, Cameron M, Shi J,
            Baran P, Fromentin R, Chomont N, Valente ST
  TITLE     An Analog of the Natural Steroidal Alkaloid Cortistatin A Potently 
            Suppresses Tat-Dependent HIV Transcription.
  JOURNAL   Cell Host Microbe. 2012 Jul 19;12(1):97-108.
  PUBMED    22817991
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2012-07-19"; volume: "12(1)"; pages: "97-108"
COMMENT     Sequence Label: pcDNA3.1-TAT-1-101-FLAG
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..64)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(96..119)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      425..434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(460..479)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(461..479)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(524..727)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(553..573)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtgaggtgag atgaccggta cgcgtagaat cgagaccgag gagagggtta gggataggct
       61 taccttcgaa ccgcgggccc tctagactgc gtctacttgt catcgtcgtc cttgtagtcg
      121 cctccatcga atggatctgt ctctgtctct ctctccacct tcttcttcga ttccttcggg
      181 cctgtcgggt cccctcggga ttgggaggtg ggttgctttg atagagaaac ttgatgagtc
      241 tgactgcctt gaggaggtct tcgtcgctgt ctccgcttct tcctgccata ggagatgcct
      301 aaggcttttg ttatgaaaca aacttggcaa tgaaagcaac actttttaca atagcaattg
      361 gtacaagcag ttttaggctg acttcctgga tgcttccagg gctctagtct aggatctact
      421 ggctccatgg tggcaagctt aactagccag cttgggtctc cctatagtga gtcgtattaa
      481 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
      541 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      601 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      661 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
      721 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
      781 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
      841 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt tacgtaatac
      901 tcaccatgac tcaaggtcaa tggaaagtcc ctattggcgg tactatggga acatacgtca
//
