LOCUS       sequence-45221-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Mousseau G, Clementz MA, Bakeman WN, Nagarsheth N, Cameron M, Shi J,
            Baran P, Fromentin R, Chomont N, Valente ST
  TITLE     An Analog of the Natural Steroidal Alkaloid Cortistatin A Potently 
            Suppresses Tat-Dependent HIV Transcription.
  JOURNAL   Cell Host Microbe. 2012 Jul 19;12(1):97-108.
  PUBMED    22817991
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2012-07-19"; volume: "12(1)"; pages: "97-108"
COMMENT     Sequence Label: pcDNA3.1-TAT-1-delta2-26-FLAG
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..127)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(390..409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(391..409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(454..657)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(483..503)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tatcatggtg aggtgatgat gaccggtacg cgtagaatcg agaccgagga gagggttagg
       61 gataggctta tcttcgaacc gcgggccctc tagactgcgt ctacttgtca tcgtcgtcct
      121 tgtagtcgcc tccatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttcgatt
      181 ccttcgggcc tgtcgggtcc cctcgggatt gggaggtggg ttgctttgat agagaaactt
      241 gatgagtctg actgccttga ggaggtcttc gtcgctgtct ccgcttcttc ctgccatagg
      301 agatgcctaa ggcttttgtt atgaaacaaa cttggcaatg aaagcaacac tttttacaca
      361 tggtaagctt aactagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag
      421 ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg
      481 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      541 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      601 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      661 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      721 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      781 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      841 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
      901 caatgggcgg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgt
//
