LOCUS       sequence-452216-       18551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for 
            monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was 
            driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant 
            select..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18551)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 18551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT29
FEATURES             Location/Qualifiers
     source          1..18551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2628..2693
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2700..2720
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2745..2768,2769..2771,2772..6845)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6758..6766
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6846..6893
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7110..7175
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7809..7829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7836..8087
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8088..8141
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8316..8369
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8376..8396
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8424..8810
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8859..9107
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9120..9140
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9147..9194
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      9216..9468
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9513..9533
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        9570..9947
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             complement(9918..10250)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLCHG
                     SSAQLF"
     primer_bind     10115..10131
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10141..10160
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10141..10159
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10207..10227)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10207..10225)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10246..10262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10270..10286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10294..10300,10301..10318,10319..10324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10339..10360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        10419..10437
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        10489..10507
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     protein_bind    complement(10626..10650)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10718..10734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10742..10758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10766..10772,10773..10790,10791..10796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10811..10832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11023..11699
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11636..11656
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11744..12295
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    12302..12476
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12554..12578
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13003..13797
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13884..14472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14373..14392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14626..14643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14658..14798
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14724..14743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14884..14906
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15142..15336
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15402..16475)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16904..17533)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     1381 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag caggctccga attcgccctt caccatggat tacaaggacc
     2641 acgacgggga ttacaaggac cacgacattg attacaagga tgatgatgac aagatggctc
     2701 cgaagaagaa gaggaaggtt ggcatccacg gggtgccagc tgctgacaag aagtactcga
     2761 tcggcctcgc tattgggact aactctgttg gctgggccgt gatcaccgac gagtacaagg
     2821 tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc aagaagaatc
     2881 tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg ctcaagcgca
     2941 ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag gagattttct
     3001 ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag tcattcctcg
     3061 tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc gacgaggttg
     3121 cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg gactccacag
     3181 ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag ttcaggggcc
     3241 atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag ctgttcatcc
     3301 agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg
     3361 acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag aacctgatcg
     3421 cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg
     3481 ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag ctgcagctct
     3541 ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc gatcagtacg
     3601 cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct gatattctca
     3661 gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag cgctacgacg
     3721 agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc cccgagaagt
     3781 acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt gacggcgggg
     3841 cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg gacggcacgg
     3901 aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg acattcgata
     3961 acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg aggaggcagg
     4021 aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt ctgactttca
     4081 ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct tggatgaccc
     4141 gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac aagggcgcta
     4201 gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca aatgagaagg
     4261 tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag ctgactaagg
     4321 tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag cagaagaagg
     4381 ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg
     4441 actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct
     4501 tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat aaggacttcc
     4561 tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc actctgttcg
     4621 aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc gatgacaagg
     4681 tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc aggaagctca
     4741 tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg aagagcgatg
     4801 gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca ttcaaggagg
     4861 atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat atcgcgaacc
     4921 tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt gtggacgagc
     4981 tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg gcccgggaga
     5041 atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg atcgaggagg
     5101 gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac acgcagctgc
     5161 agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat gtggaccagg
     5221 agctggatat taacaggctc agcgattacg acgtcgatca tatcgttcca cagtcattcc
     5281 tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt
     5341 ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg cgccagctcc
     5401 tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct gagaggggcg
     5461 ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag acacggcaga
     5521 tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac gatgagaatg
     5581 acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc tccgacttca
     5641 ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat gcccatgacg
     5701 cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg
     5761 agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc
     5821 aggagattgg caaggctacc gccaagtact tcttctactc taacattatg aatttcttca
     5881 agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc gagacgaacg
     5941 gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc aggaaggttc
     6001 tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc gggttctcta
     6061 aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag aaggactggg
     6121 atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg
     6181 cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg ctggggatca
     6241 cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag gcgaagggct
     6301 acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc ttcgagctgg
     6361 agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg aacgagctgg
     6421 ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag aagctcaagg
     6481 gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag cattacctcg
     6541 acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc
     6601 tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg gagcaggctg
     6661 agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct ttcaagtact
     6721 tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg gacgcgaccc
     6781 tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct cagctcgggg
     6841 gcgacaagcg gccagcggcg acgaagaagg cggggcaggc gaagaagaag aagggagacg
     6901 acggaggtgg cggtacagga gggggtgggt ccgctgagta tgtcagggcg ttgttcgact
     6961 tcaatggaaa cgacgaggaa gatctgcctt ttaaaaaggg agatattctc aggatcagag
     7021 ataagccgga agaacaatgg tggaacgctg aagactctga aggtaagaga ggtatgattc
     7081 ttgtccccta cgtcgagaag tattcgggtg actataaaga ccacgatgga gattataagg
     7141 accacgatat agattataag gatgatgatg ataagagcgg aatgaccgat gcagagtacg
     7201 tcaggattca tgagaaactt gacatctaca cgtttaagaa acagtttttc aacaacaaaa
     7261 aatctgttag tcaccgctgt tacgtgctgt tcgaattgaa acgcagaggt gagaggagag
     7321 cctgcttttg gggctatgcc gtcaacaagc cgcaaagcgg cacagaaagg ggcattcacg
     7381 cggagatatt tagcattaga aaggtcgagg aataccttcg ggataatccc gggcaattca
     7441 ctatcaattg gtactcttca tggtccccgt gtgcagattg cgctgaaaag atactggagt
     7501 ggtataatca agaactcaga ggaaacggtc acaccctcaa gatttgggct tgcaagcttt
     7561 actacgagaa aaatgcaagg aaccagatcg gcctctggaa cttgcgcgac aacggcgtgg
     7621 ggttgaatgt gatggtgtcg gagcattacc agtgctgccg gaagatattc attcagtcgt
     7681 cacataatca attgaacgag aataggtggc tcgaaaaaac cctgaagcgg gccgagaagt
     7741 ggaggagtga actctcgata atgatccagg ttaaaatact gcatactacc aaatctccgg
     7801 cggtgggacc gaagaagaag cgcaaggtgg ggaccatgac taatctctca gatataatcg
     7861 agaaggaaac aggaaagcaa ctggtcatcc aagaatcgat tttgatgctt cccgaagaag
     7921 tcgaagaagt tataggaaat aagcccgagt ctgacatact ggttcacaca gcgtacgatg
     7981 aaagtacgga cgagaatgtc atgttgctga catcggacgc acctgaatac aagccttggg
     8041 ctctggtcat acaagatagt aacggagaaa ataagattaa aatgcttgaa ggaaggggct
     8101 ccctgctgac atgtggagat gtggaagaga atccaggacc atccaggatg atagagcaag
     8161 atggttgtac ccaagtgctg cggcccctcg gatggcgggg gtacagcgcc atgactggcc
     8221 ataatagaca gtccgctgct ctcatgcctc catgcagcgg atgtcagagg aggggagcta
     8281 ggttcttcct gtctagacct acctgccccg tgcccgaggg cagaggaagt cttctaacat
     8341 gcggtgacgt ggaggagaat cccggcccta tggctcctaa gaagaagcgg aaggttggta
     8401 ttcacggggt gcctgcggct atggcgtcaa atttcacgca gtttgttttg gttgataacg
     8461 gcgggactgg cgacgttaca gtagctccat caaattttgc gaacggagtc gctgagtgga
     8521 ttagctcaaa ttcaaggtcc caggcctaca aggttacctg ttctgttagg cagagttctg
     8581 cgcaaaaaag aaaatatacc atcaaggttg aagtccctaa agttgcaaca caaacagtcg
     8641 gtggtgttga gctccctgtg gcagcctgga gatcttactt aaacatggag ctaacaattc
     8701 caatattcgc tacaaactct gattgtgaac tgattgttaa ggcgatgcaa ggactcttga
     8761 aagatggaaa ccctataccg tccgctatcg cagctaacag cggtatctat ggctctggat
     8821 cggggtcggg ttctggctca actagttcag gcgggtcaac aaacctcagt gacatcatag
     8881 agaaggaaac tggtaagcaa ctggttattc aagagagcat tctgatgctc cctgaggaag
     8941 tcgaggaagt tataggaaac aagcctgaga gcgatatact cgtgcatacg gcgtatgacg
     9001 agagcacgga tgaaaatgtg atgctcttga ccagcgatgc gccagaatat aaaccctggg
     9061 cactggttat tcaggactct aacggggaaa ataaaatcaa gatgctctca ggtggctccc
     9121 caaagaagaa acgcaaggtt ggtaccaagc gtcctgctgc caccaaaaag gccggacagg
     9181 ctaagaaaaa gaagtagtgg cggccgccga cgtccgatcg ttcaaacatt tggcaataaa
     9241 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     9301 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     9361 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     9421 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc
     9481 cccctcgaca gcttccggaa agggcgaatt cgcaactttg tatacaaaag ttgccccatg
     9541 gcgttccctc tagataacgc aggatcctaa agggatcttt aaacatacga acagatcact
     9601 taaagttctt ctgaagcaac ttaaagttat caggcatgca tggatcttgg aggaatcaga
     9661 tgtgcagtca gggaccatag cacaggacag gcgtcttcta ctggtgctac cagcaaatgc
     9721 tggaagccgg gaacactggg tacgttggaa accacgtgat gtggagtaag ataaactgta
     9781 ggagaaaagc atttcgtagt gggccatgaa gcctttcagg acatgtattg cagtatgggc
     9841 cggcccatta cgcaattgga cgacaacaaa gactagtatt agtaccacct cggctatcca
     9901 catagatcaa agctggttta aaagagttgt gcagatgatc cgtggcagag accggcgccg
     9961 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    10021 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    10081 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
    10141 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt
    10201 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
    10261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10381 cgctttccac cggtggtctc tgttttagag ctaggccaac atgaggatca cccatgtctg
    10441 cagggcctag caagttaaaa taaggctagt ccgttatcaa cttggccaac atgaggatca
    10501 cccatgtctg cagggccaag tggcaccgag tcggtgcttt ttttgtccct tcgaagggca
    10561 attctgcaga tatccatcac actggcggcc gctcgaggtc gagggtatcg ataagcttga
    10621 attcgaccca gctttcttgt acaaagtggt tcgataattc ttaattaact agttctagag
    10681 cggccgccac cgcggtggag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa
    10741 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10801 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10861 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10921 gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc
    10981 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
    11041 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
    11101 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
    11161 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
    11221 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg
    11281 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
    11341 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
    11401 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
    11461 atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag
    11521 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
    11581 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
    11641 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
    11701 atcaccagtc tctctctaca aatctatctc tctcgagtct accatgagcc cagaacgacg
    11761 cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa
    11821 ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg
    11881 gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg
    11941 cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct acgactggac
    12001 ggccgagtcg accgtgtacg tctccccccg ccaccagcgg acgggactgg gctccacgct
    12061 ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg tcgctgtcat
    12121 cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg
    12181 catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt tctggcagct
    12241 ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga tttgactcga
    12301 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
    12361 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
    12421 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc
    12481 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
    12541 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
    12601 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
    12661 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
    12721 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
    12781 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
    12841 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
    12901 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
    12961 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
    13021 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
    13081 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
    13141 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
    13201 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
    13261 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
    13321 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
    13381 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
    13441 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
    13501 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
    13561 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
    13621 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
    13681 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
    13741 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
    13801 ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    13861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    13921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    13981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    14041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    14101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    14161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    14221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    14281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    14341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    14401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    14461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    14521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    14581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    14641 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    14701 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    14761 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
    14821 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    14881 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
    14941 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
    15001 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
    15061 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
    15121 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
    15181 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
    15241 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    15301 tacggctttg ggttcccaat gtacgtgcta tccacaggaa acagaccttt tcgacctttt
    15361 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    15421 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    15481 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    15541 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    15601 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    15661 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    15721 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    15781 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    15841 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    15901 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    15961 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    16021 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    16081 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    16141 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    16201 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    16261 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    16321 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    16381 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    16441 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    16501 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
    16561 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    16621 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    16681 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    16741 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    16801 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    16861 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    16921 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    16981 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    17041 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    17101 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    17161 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    17221 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    17281 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    17341 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    17401 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    17461 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    17521 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    17581 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    17641 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    17701 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    17761 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    17821 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    17881 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    17941 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    18001 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    18061 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    18121 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    18181 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    18241 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    18301 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    18361 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    18421 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    18481 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    18541 tcgcaagtat t
//
