LOCUS       sequence-452223-       16201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for dLbCas12a-D156R mediated A to G base editing for 
            monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were 
            driven by separate 2x35s promoter; Kanamycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16201)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 16201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT54
FEATURES             Location/Qualifiers
     source          1..16201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3897)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4326..4955)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6256..6280
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6483..6499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6926..7270
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7330..7354
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7394..7414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8042..8062
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11768..11815
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11846..12098
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    12143..12163
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        12602..12946
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     ncRNA           13005..13041
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(13051..13365)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLIYT
                     "
     primer_bind     13230..13246
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13256..13275
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13256..13274
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13322..13342)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13322..13340)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13361..13377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13385..13401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13409..13415,13416..13433,13434..13439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13454..13475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     ncRNA           13517..13584
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(13604..13628)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    13962..14018
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(14027..14043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    14051..14067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14075..14081,14082..14099,14100..14105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14120..14141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        14332..15008
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             15071..15868
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15128..15147)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15738..15757
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15925..16099
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    16177..16201
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccaa gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     3901 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     3961 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     4021 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     4081 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     4141 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     4201 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     4261 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     4321 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     4381 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     4441 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     4501 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     4561 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     4621 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     4681 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     4741 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     4801 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     4861 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     4921 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     4981 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     5041 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     5101 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     5161 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     5221 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     5281 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     5341 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     5401 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     5461 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     5521 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     5581 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     5641 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     5701 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     5761 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     5821 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     5881 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     5941 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     6001 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     6061 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     6121 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     6181 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     6241 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     6301 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     6361 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6421 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6481 ttgtaaaacg acggccagtg ccaagcttgg cgtgcctgca ggtcaacatg gtggagcacg
     6541 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     6601 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     6661 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     6721 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     6781 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     6841 attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     6901 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     6961 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     7021 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     7081 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     7141 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     7201 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     7261 tggagaggac ctcgactcta gaggatcccc gggtaccggg ccccccctcg aggcgcgcca
     7321 agctatcaaa caagtttgta caaaaaagca ggctccgatg aaacggacag ccgacggaag
     7381 cgagttcgag tcaccaaaga agaagcggaa agtctctgag gtggagtttt cccacgagta
     7441 ctggatgaga catgccctga ccctggccaa gagggcacgg gatgagaggg aggtgcctgt
     7501 gggagccgtg ctggtgctga acaatagagt gatcggcgag ggctggaaca gagccatcgg
     7561 cctgcacgac ccaacagccc atgccgaaat tatggccctg agacagggcg gcctggtcat
     7621 gcagaactac agactgattg acgccaccct gtacgtgaca ttcgagcctt gcgtgatgtg
     7681 cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg tttggcgtga ggaactcaaa
     7741 aagaggcgcc gcaggctccc tgatgaacgt gctgaactac cccggcatga atcaccgcgt
     7801 cgaaattacc gagggaatcc tggcagatga atgtgccgcc ctgctgtgcg atttctatcg
     7861 gatgcctaga caggtgttca atgctcagaa gaaggcccag agctccatca acggaggggg
     7921 agggtcggga ggtggtggca gcggaggagg cggtagcgga ggtggaggat caggtggcgg
     7981 agggtcggga gggggcggtt ctggtggtgg gggctcgggt ggaggaggga gcaccatggc
     8041 tcctaagaag aagcggaagg ttggtattca cggggtgcct gcggcttcaa agctcgagaa
     8101 attcaccaac tgttattcgt tgagcaaaac actgcggttt aaagcgattc cagtcggcaa
     8161 gactcaagag aatatagaca ataagcggct gttggtggaa gatgaaaagc gcgcggaaga
     8221 ctacaaaggg gtgaagaagt tgttggacag atactacctc tcttttatca atgatgtctt
     8281 gcactcaatc aaattgaaga atctgaacaa ctacatctcc ctcttcagaa agaaaacaag
     8341 gacagaaaag gagaataagg aacttgaaaa tttggagatc aatctgagga aagagatcgc
     8401 gaaagccttt aaaggcaacg aaggatacaa aagtctgttc aagaaggata taattgagac
     8461 aattttgcca gagttcctcg atgacaagga cgagattgcg ctggtcaatt cgttcaacgg
     8521 attcacaaca gcattcacag gcttctttcg caatcgggaa aatatgttct ctgaggaggc
     8581 aaagtccact tctattgcgt tcaggtgtat caatgagaat ctcactaggt acatttccaa
     8641 catggatatc tttgagaagg ttgacgcaat ttttgacaag cacgaagttc aggagattaa
     8701 ggagaagatc ctcaattccg attatgacgt tgaggacttc ttcgaaggtg agttttttaa
     8761 tttcgtgctc actcaagagg gtatcgacgt gtataatgcg atcatcggtg ggttcgtgac
     8821 tgagtccggt gaaaagatta agggattgaa cgagtatatc aacctttaca accaaaagac
     8881 gaaacagaag ctgccaaagt tcaagcctct ttacaaacag gttctttcag accgcgagtc
     8941 actctcgttc tatggggagg gctacacttc ggatgaggaa gtcctggagg tgttcaggaa
     9001 tactctcaat aagaattcgg agattttctc ttctataaaa aaactggaaa agttgtttaa
     9061 gaattttgac gaatactcta gcgccggcat atttgtgaaa aacggcccgg ccatatcaac
     9121 gataagtaaa gatatcttcg gcgaatggaa cgtgatcaga gacaaatgga acgcggagta
     9181 tgacgatatt cacctgaaga agaaggctgt cgtaacggag aagtacgagg atgatcgcag
     9241 gaaaagcttc aaaaagatcg gaagtttcag cctggaacag ttgcaggagt atgctgacgc
     9301 cgatcttagc gtcgtcgaga agttgaagga gataatcatc caaaaggtcg acgagatata
     9361 taaagtctat ggatcaagtg aaaaactgtt cgacgccgac ttcgttttgg agaagtccct
     9421 gaagaagaac gacgctgttg ttgccattat gaaggatctg ctcgacagcg tgaagagttt
     9481 cgagaactat attaaggctt ttttcgggga ggggaaggag actaacagag atgagtcctt
     9541 ctacggagac ttcgtcctcg cgtacgatat actccttaag gtagaccaca tctacgacgc
     9601 aatcagaaat tacgtgacac aaaagccgta cagcaaggac aagttcaaac tctacttcca
     9661 gaacccccag ttcatgggcg gctgggacaa ggacaaggaa acggattaca gggctacgat
     9721 cctgaggtat ggttcaaaat actacttggc gattatggac aagaagtacg ccaagtgtct
     9781 ccagaagatt gacaaagacg atgtcaatgg caattatgag aagatcaact acaagctgct
     9841 tccgggtccg aacaagatgc tcccaaaggt tttcttcagc aagaaatgga tggcctacta
     9901 taacccaagc gaggacatcc agaagattta taagaacggt acgttcaaga agggcgacat
     9961 gttcaatctt aacgactgtc acaagctgat cgacttcttc aaagactcaa ttagccggta
    10021 cccaaagtgg tctaacgcct atgacttcaa cttttcggaa accgagaagt acaaggatat
    10081 agccggattt tatagagagg tggaagagca gggctacaag gtgtcattcg agtccgccag
    10141 caagaaggaa gtggacaagc tcgtggaaga gggtaagctc tacatgttcc agatttataa
    10201 taaagacttt agcgataaga gccacgggac acctaatctc cacacaatgt atttcaagct
    10261 gctcttcgac gagaataacc acggccaaat caggttgtca ggaggggctg aactcttcat
    10321 gcggcgcgct agccttaaga aggaggagct tgtagtccac cctgcgaata gtccaattgc
    10381 gaataagaac ccggacaatc ctaaaaagac tacaacattg agctacgacg tgtacaagga
    10441 taagaggttt tccgaggatc agtacgagct ccacatcccg attgcgatca acaagtgccc
    10501 aaagaatatt ttcaagataa acacagaggt gcgtgtactc ctgaagcatg acgacaatcc
    10561 ttacgtcatt gggattgccc ggggcgagag gaacctcctc tatattgtgg tggtggacgg
    10621 gaaggggaac atagtcgaac agtactccct taacgaaata attaacaatt tcaacggcat
    10681 ccgtatcaag accgactacc attcgttgct ggacaagaag gagaaggaga gatttgaggc
    10741 gcggcaaaat tggacaagta tcgagaacat caaggaactc aaagcaggtt atatctctca
    10801 agttgtgcat aagatatgcg agctggttga gaagtatgac gcagtgatcg ctcttgagga
    10861 cctcaactcg ggctttaaga attctagagt taaagtggag aagcaggtct atcaaaagtt
    10921 cgagaagatg cttatagata agctcaacta catggtcgat aagaaatcga acccatgtgc
    10981 caccggcggc gcactcaaag gttaccaaat aacaaacaaa ttcgagtcct tcaaatcgat
    11041 gagtactcag aatgggttca tattttatat accggcgtgg cttacgtcta agatcgaccc
    11101 gtcaactggt tttgtcaacc tgttgaagac gaaatacacg tccattgccg attcgaaaaa
    11161 gttcatatct agttttgatc gtattatgta cgtcccagag gaagatcttt tcgagtttgc
    11221 tctcgactac aaaaactttt cgcggaccga tgcggattac attaaaaaat ggaaactcta
    11281 ttcgtacggc aacagaatca ggatttttcg caaccctaag aagaataacg tctttgattg
    11341 ggaggaagtt tgcttgacta gcgcgtacaa ggagctcttt aataagtatg gcattaacta
    11401 ccaacagggt gatatcagag cactgctttg cgaacaatct gacaaggctt tctactcatc
    11461 cttcatggct ttgatgagcc tgatgctcca gatgagaaat tcaattacag gcagaaccga
    11521 cgtggatttc ttgatctccc cggttaaaaa ttctgatggc atcttttacg atagcaggaa
    11581 ctatgaagcg caagagaatg cgattctgcc aaaaaatgca gacgccaacg gtgcctataa
    11641 catcgccagg aaagtcctgt gggcgatcgg ccagttcaaa aaggccgaag acgaaaaatt
    11701 ggacaaggtc aaaatcgcta tcagcaacaa agagtggctg gagtatgctc agacatccgt
    11761 aaagcataag cgtcctgctg ccaccaaaaa ggccggacag gctaagaaaa agaagtgaga
    11821 cgactagtgg cggccgccga cgtccgatcg ttcaaacatt tggcaataaa gtttcttaag
    11881 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
    11941 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
    12001 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
    12061 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc cccctcgaca
    12121 gcttccggaa agggcgaatt cgcaactttg tatacaaaag ttgccccatg gcgttccctc
    12181 tagtggcgtg cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata
    12241 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
    12301 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
    12361 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
    12421 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
    12481 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc
    12541 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
    12601 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
    12661 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
    12721 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
    12781 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
    12841 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
    12901 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg actctagagg
    12961 atccccgggt acgtttggtg ttacttctgc agggatccaa attactgatg agtccgtgag
    13021 gacgaaacga gtaagctcgt ctaatttcta ctaagtgtag atgagaccgg cgccgctaca
    13081 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    13141 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    13201 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
    13261 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc agcttttgtt
    13321 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    13381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    13441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    13501 tccaccggtg gtctctggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac
    13561 atgcttcggc atggcgaatg ggacggtacc cggccggaat tcgacccagc tttcttgtac
    13621 aaagtggttc gataattctt aattaactag ttctagagcg gccgccaccg cggtggagct
    13681 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
    13741 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
    13801 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
    13861 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
    13921 cggtgtcatc tatgttactg aattcgtaat catggtcata gaagcttgca tgcctgcagg
    13981 tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
    14041 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    14101 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    14161 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    14221 ggagaggcgg tttgcgtatt ggctagagca gcttgccaac atggtggagc acgacactct
    14281 cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt
    14341 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
    14401 catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg
    14461 aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
    14521 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
    14581 tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
    14641 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
    14701 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
    14761 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
    14821 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
    14881 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
    14941 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
    15001 cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatctg
    15061 tcgatcgacc atggggattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    15121 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    15181 gtttcggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    15241 cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    15301 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    15361 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    15421 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    15481 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    15541 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    15601 gcgcatgccc gacggcgagg atctcgtcgt gacacatggc gatgcctgct tgccgaatat
    15661 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    15721 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    15781 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    15841 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcggatc gatcctctag
    15901 ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa
    15961 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta
    16021 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
    16081 cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt
    16141 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
    16201 a
//
