LOCUS       sequence-452225-       15606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; 
            more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were 
            driven by separate 2x35s promoter; Kanamycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 15606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT48
FEATURES             Location/Qualifiers
     source          1..15606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3897)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4326..4955)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6256..6280
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6483..6499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6926..7270
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7330..7354
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7379..7399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11174..11221
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11252..11504
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    11549..11569
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        12008..12352
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     ncRNA           12411..12447
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             complement(12447..12770)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLIYN
                     SRN"
     primer_bind     12635..12651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12661..12680
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12661..12679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12727..12747)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12727..12745)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12766..12782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12790..12806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12814..12820,12821..12838,12839..12844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12859..12880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     ncRNA           12922..12989
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(13009..13033)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    13367..13423
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(13432..13448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    13456..13472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13480..13486,13487..13504,13505..13510))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13525..13546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13737..14413
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             14476..15273
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14533..14552)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15143..15162
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15330..15504
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    15582..15606
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaacagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccaa gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     3901 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     3961 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     4021 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     4081 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     4141 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     4201 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     4261 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     4321 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     4381 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     4441 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     4501 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     4561 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     4621 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     4681 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     4741 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     4801 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     4861 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     4921 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     4981 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     5041 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     5101 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     5161 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     5221 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     5281 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     5341 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     5401 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     5461 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     5521 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     5581 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     5641 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     5701 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     5761 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     5821 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     5881 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     5941 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     6001 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     6061 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     6121 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     6181 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     6241 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     6301 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     6361 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6421 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6481 ttgtaaaacg acggccagtg ccaagcttgg cgtgcctgca ggtcaacatg gtggagcacg
     6541 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     6601 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     6661 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     6721 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     6781 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     6841 attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     6901 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     6961 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     7021 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     7081 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     7141 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     7201 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     7261 tggagaggac ctcgactcta gaggatcccc gggtaccggg ccccccctcg aggcgcgcca
     7321 agctatcaaa caagtttgta caaaaaagca ggctccgaat tcgcccttca ccatggctcc
     7381 taagaagaag cggaaggttg gtattcacgg ggtgcctgcg gctctgtttc aagattttac
     7441 acatctgtac ccgctgagta aaacagtgcg gttcgagctg aaacccatag gaaggaccct
     7501 cgagcacatc cacgcgaaga attttctgag ccaggatgaa actatggctg atatgtatca
     7561 aaaagttaag gtcattttgg acgactatca tcgcgatttt attgccgaca tgatgggaga
     7621 ggtgaaactc acgaagcttg ctgaatttta cgacgtctat ctgaagttca ggaaaaatcc
     7681 taaggacgat gggctgcaaa aacagcttaa agaccttcaa gctgtccttc ggaaggaatc
     7741 ggtgaagcct atagggtcag gtgggaagta caaaacaggc tacgatagac tctttggggc
     7801 aaaactcttc aaagatggaa aagagttggg tgacctcgca aaattcgtta tagcccaaga
     7861 aggtgagtct tctccgaagc tggctcatct tgctcatttt gagaagttca gcacgtattt
     7921 tactggattt cacgataatc ggaagaatat gtactcggat gaagacaagc atactgcaat
     7981 agcgtacagg ctcatccatg agaatttgcc gagattcatc gacaatctgc aaatcttgac
     8041 aacaatcaaa caaaagcata gcgccctcta tgatcagata atcaacgagc tcacggcctc
     8101 cgggctcgac gtctccttgg cttctcatct tgacgggtat cacaagctcc ttacacaaga
     8161 ggggatcacg gcatacaaca ggatcatagg agaggtgaat ggatatacaa ataagcataa
     8221 ccagatatgc cacaagagcg agcgcatagc gaaacttaga cccttgcaca agcaaatcct
     8281 ttctgacgga atgggagtgt cattccttcc gtctaagttc gcggatgata gtgagatgtg
     8341 ccaagcggtc aacgaatttt atcgccatta tactgacgtg ttcgcaaagg tgcaaagtct
     8401 ctttgacgga tttgatgatc accagaaaga cgggatctat gttgaacaca aaaaccttaa
     8461 tgaactgagc aaacaggcgt tcggcgactt tgctttgctg gggagggtcc ttgatggata
     8521 ctacgtggac gttgtcaatc cggagttcaa tgagcggttc gcaaaggcca agactgacaa
     8581 tgcgaaagcc aagcttacaa aagaaaagga caaattcatt aaaggagtcc actcactggc
     8641 ttccctcgaa caagcaatag aacaccatac agctagacac gacgatgaga gtgttcaagc
     8701 cggaaaactt ggccagtact tcaaacacgg tttggcgggg gttgacaacc cgattcagaa
     8761 aattcacaat aaccattcga cgattaaagg gtttctggaa agggaaaggc ctgctgggga
     8821 acgggcgctc ccgaagatca agtcaggaaa aaacccagaa atgacacagc tcaggcagct
     8881 gaaggaactt ttggacaacg cattgaatgt ggcgcacttc gctaagctgc tgacaactaa
     8941 aacaaccttg gacaaccagg atggaaattt ttacggggag tttggggtgc tttacgacga
     9001 gctggctaaa attccaactc tctacaataa ggttagagat tatctctctc aaaagccctt
     9061 ttctaccgaa aagtataagc tcaacttcgg caatccgacc cttctccgcg ggtgggacct
     9121 gaacgtggag aaagatcgct ttggggttat acttcagaag gatggatgct attacttggc
     9181 gcttcttgat aaggctcata aaaaagtttt cgacaacgcc cctaacactg gtaagaacgt
     9241 ctaccaaaag atggtctaca aactgttgcc cggccccaac aaaatgcttc ctagggtgtt
     9301 tttcgcaaaa tcgaatctcg actattataa tccatctgcc gagctccttg acaaatatgc
     9361 taaggggacc cataaaaagg gtgataattt caacctgaag gactgccacg cgcttatcga
     9421 ctttttcaaa gccgggataa ataagcatcc ggagtggcaa cattttggtt ttaaattttc
     9481 gccaacgtcg tcctatcgcg acctttccga tttctatagg gaagttgaac ctcaggggta
     9541 ccaggtcaaa tttgttgaca ttaatgcgga ctacattgat gaattggtgg agcaagggaa
     9601 gctctacctc tttcaaatat ataacaaaga tttctcgcca aaagcgcatg gtaaaccgaa
     9661 tcttcatacc ttgtacttta aagcactttt ttcagaagat aacttggcgg acccgatcta
     9721 caagctgaat ggggaagctc agatcttcta caggaaagct tcgttggaca tgaacgagac
     9781 taccatacat cgcgcgggag aggtgcttga gaacaaaaat cccgacaacc cgaaaaagcg
     9841 gcaattcgtt tacgacatca tcaaagacaa acggtacacg caggacaaat ttatgctcca
     9901 cgtccccatt accatgaatt ttggagtcca aggcatgacc attaaggaat tcaacaaaaa
     9961 ggtcaaccaa agtattcagc aatacgatga agtcaatgtc ataggcatag atcggggaga
    10021 aaggcatctg ttgtatctta ccgtgattaa ctctaagggt gaaatactgg agcaacggtc
    10081 acttaacgat ataaccacgg cgtccgcgaa cggtacacaa gtgaccactc cctaccacaa
    10141 aatattggat aaaagggaga tagaacgctt gaatgcccgc gttggctggg gtgagattga
    10201 gacgatcaaa gagcttaaat cgggatattt gtctcacgtc gttcatcaaa ttaaccaact
    10261 catgcttaag tacaatgcaa tcgttgtgct cgaggacctg aactttggtt tcaaaagagg
    10321 gaggttcaag gtggaaaaac aaatttacca gaactttgaa aacgcgctta tcaagaaatt
    10381 gaatcacctt gttttgaaag ataaggcaga tgacgaaatc gggtcgtata aaaatgcact
    10441 ccagttgaca aataatttca cggatttgaa gtcgatcggc aagcaaacag ggttcctctt
    10501 ttatgtgcca gcgtggaata catcaaaaat tgatccggag acgggatttg tcgacttgct
    10561 gaagcctagg tatgagaaca ttgcccaatc tcaggccttt ttcggcaaat tcgataaaat
    10621 atgctacaac acagacaaag gttattttga atttcacatt gattacgcca aatttacaga
    10681 taaggcgaaa aacagcagac agaaatgggc tatctgttct catggggaca aacgctatgt
    10741 ctacgataag acggctaatc aaaataaagg cgccgcaaaa ggtattaatg tgaatgatga
    10801 gctgaaaagc ttgtttgccc gctaccatat caatgataaa caaccaaact tggtgatgga
    10861 catatgccag aacaatgaca aagaattcca caagtcactc atgtgcctgc ttaaaaccct
    10921 tttggcgctg cggtatagca atgcatctag cgatgaagac tttattttga gtcccgtggc
    10981 caacgacgag ggcgtgtttt ttaattcagc cttggcggac gatacgcagc cccagaatgc
    11041 ggacgcaaac ggcgcgtacc acattgcact gaagggactg tggcttctga acgagctgaa
    11101 aaatagcgac gacctgaata aagtcaagtt ggccattgac aatcaaacct ggttgaattt
    11161 cgctcaaaat agaaagcgtc ctgctgccac caaaaaggcc ggacaggcta agaaaaagaa
    11221 gtgagacgac tagtggcggc cgccgacgtc cgatcgttca aacatttggc aataaagttt
    11281 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
    11341 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
    11401 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
    11461 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tgatcccccc
    11521 tcgacagctt ccggaaaggg cgaattcgca actttgtata caaaagttgc cccatggcgt
    11581 tccctctagt ggcgtgcctg caggtcaaca tggtggagca cgacacactt gtctactcca
    11641 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
    11701 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
    11761 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
    11821 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
    11881 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg
    11941 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
    12001 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
    12061 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
    12121 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
    12181 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
    12241 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
    12301 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acctcgactc
    12361 tagaggatcc ccgggtacgt ttggtgttac ttctgcaggg atccaaatta ctgatgagtc
    12421 cgtgaggacg aaacgagtaa gctcgtctaa tttctactgt tgtagatgag accggcgccg
    12481 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    12541 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    12601 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
    12661 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt
    12721 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
    12781 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    12841 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    12901 cgctttccac cggtggtctc tggccggcat ggtcccagcc tcctcgctgg cgccggctgg
    12961 gcaacatgct tcggcatggc gaatgggacg gtacccggcc ggaattcgac ccagctttct
    13021 tgtacaaagt ggttcgataa ttcttaatta actagttcta gagcggccgc caccgcggtg
    13081 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
    13141 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
    13201 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
    13261 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
    13321 gcgcgcggtg tcatctatgt tactgaattc gtaatcatgg tcatagaagc ttgcatgcct
    13381 gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca tgtcatagct
    13441 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    13501 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    13561 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    13621 cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac
    13681 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    13741 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    13801 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    13861 aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca
    13921 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    13981 tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    14041 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    14101 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    14161 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
    14221 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    14281 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    14341 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    14401 gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag
    14461 atctgtcgat cgaccatggg gattgaacaa gatggattgc acgcaggttc tccggccgct
    14521 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    14581 gccgtgtttc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    14641 ggtgccctga atgaactcca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
    14701 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
    14761 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    14821 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    14881 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
    14941 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    15001 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg
    15061 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    15121 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    15181 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    15241 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc ggatcgatcc
    15301 tctagctaga gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca
    15361 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
    15421 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
    15481 aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa
    15541 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
    15601 ctgcca
//
