LOCUS       sequence-45223-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Mousseau G, Clementz MA, Bakeman WN, Nagarsheth N, Cameron M, Shi J,
            Baran P, Fromentin R, Chomont N, Valente ST
  TITLE     An Analog of the Natural Steroidal Alkaloid Cortistatin A Potently 
            Suppresses Tat-Dependent HIV Transcription.
  JOURNAL   Cell Host Microbe. 2012 Jul 19;12(1):97-108.
  PUBMED    22817991
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2012-07-19"; volume: "12(1)"; pages: "97-108"
COMMENT     Sequence Label: pcDNA3.1-TAT-2-130-FLAG
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..62)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(94..117)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(536..555)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(537..555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(600..803)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(629..649)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aggtgatgat gaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
       61 ccttcgaacc gcgggccctc tagactgcgt ctacttgtca tcgtcgtcct tgtagtctcg
      121 gccagggcca gtatctgtct ccaccgttgc ttccaccgtc ttcttctgtt tcttcgttgg
      181 ctggctgtcc ccggtccttg tggatatcga tttgtctggt gtaggagacg gatgagtctt
      241 agttttcttt ggagtccttc ttcgtctgcc ctttcgttca taacatatcc cgagcccctt
      301 gtttagaaaa cacatctgac aatggtagca gcatcgctta caatagcatg agttattgca
      361 tgtttctagg ggtcggtata gctgagagag gatttcctcc ccttgtctgg ccaattcttg
      421 agtggccaca tcctgctctg aagtgcgtga aaagggctcg ttgcaggact ttaatgagct
      481 ctctggcgcc ttcaagggtg tctccatggt aagcttaact agccagcttg ggtctcccta
      541 tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga
      601 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      661 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      721 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      781 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
//
