LOCUS       sequence-45226-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Mousseau G, Clementz MA, Bakeman WN, Nagarsheth N, Cameron M, Shi J,
            Baran P, Fromentin R, Chomont N, Valente ST
  TITLE     An Analog of the Natural Steroidal Alkaloid Cortistatin A Potently 
            Suppresses Tat-Dependent HIV Transcription.
  JOURNAL   Cell Host Microbe. 2012 Jul 19;12(1):97-108.
  PUBMED    22817991
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2012-07-19"; volume: "12(1)"; pages: "97-108"
COMMENT     Sequence Label: pcDNA3.1-TAT-1-101-FLAG basic mutant
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(101..124)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(465..484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(466..484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(529..732)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(558..578)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tataggtgag gtgagatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag actgcgtcta cttgtcatcg tcgtccttgt
      121 agtcgcctcc atcgaatgga tctgtctctg tctctctctc caccttcttc ttcgattcct
      181 tcgggcctgt cgggtcccct cgggattggg aggtgggttg ctttgataga gaaacttgat
      241 gagtctgact gccttgagga ggcgctgccg cctgtgccgc cttcttcgcg ccataggaga
      301 tgcctaaggc ttttgttatg aaacaaactt ggcaatgaaa gcaacacttt ttacaatagc
      361 aattggtaca agcagtttta ggctgacttc ctggatgctt ccagggctct agtctaggat
      421 ctactggctc catggtggca agcttaacta gccagcttgg gtctccctat agtgagtcgt
      481 attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat
      541 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      601 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      661 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      721 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      781 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      841 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      901 taaatactcc acccattgac
//
