LOCUS       sequence-452305-       16862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; 
            Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter;
            BASTA for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16862)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 16862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT02
FEATURES             Location/Qualifiers
     source          1..16862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..126
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        142..2137
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2180..2204
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2226..2291
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2298..2318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2343..6443
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2343..6443
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6356..6364
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6444..6491
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    7166..7186
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(7547..7852)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     7717..7733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7743..7762
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7743..7761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7809..7829)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7809..7827)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7848..7864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7872..7888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7896..7902,7903..7920,7921..7926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7941..7962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        8004..8079
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8282..8306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8374..8390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8398..8414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8422..8428,8429..8446,8447..8452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8467..8488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     gap             8501..8600
                     /estimated_length=100
     gap             9157..9256
                     /estimated_length=100
     primer_bind     9420..9440
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        9429..9475
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             9528..10079
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    10086..10260
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10338..10362
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10787..11581
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11668..12256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12157..12176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12410..12427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12442..12582
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12508..12527)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12668..12690
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12926..13120
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13186..14259)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14688..15317)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    16618..16642
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     16845..16861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
       61 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
      121 gccagtgcca agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag
      181 ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt
      241 gtttgaagtg cagtttatct atctttatac atatatttaa actttactct acgaataata
      301 taatctatag tactacaata atatcagtgt tttagagaat catataaatg aacagttaga
      361 catggtctaa aggacaattg agtattttga caacaggact ctacagtttt atctttttag
      421 tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt
      481 ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt
      541 acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt
      601 ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa
      661 taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc
      721 cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg
      781 cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg
      841 agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga
      901 gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct
      961 acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata
     1021 gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca
     1081 accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct
     1141 cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt
     1201 agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag
     1261 cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt
     1321 ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga
     1381 ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc
     1441 gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg
     1501 gtctggttgg gcggtcgttc tagatcggag tagaattaat tctgtttcaa actacctggt
     1561 ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa
     1621 gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat
     1681 gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc
     1741 gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat
     1801 tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa
     1861 tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg
     1921 catatgcagc atctattcat atgctctaac cttgagtacc tatctattat aataaacaag
     1981 tatgttttat aattattttg atcttgatat acttggatga tggcatatgc agcagctata
     2041 tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg
     2101 tcgatgctca ccctgttgtt tggtgttact tctgcaggtc gactctagag gatcccctcg
     2161 aggcgcgcca agctatcaaa caagtttgta caaaaaagca ggctccgaat tcgcccttca
     2221 ccatggatta caaggaccac gacggggatt acaaggacca cgacattgat tacaaggatg
     2281 atgatgacaa gatggctccg aagaagaaga ggaaggttgg catccacggg gtgccagctg
     2341 ctgacaagaa gtactcgatc ggcctcgata ttgggactaa ctctgttggc tgggccgtga
     2401 tcaccgacga gtacaaggtg ccctcaaaga agttcaaggt cctgggcaac accgatcggc
     2461 attccatcaa gaagaatctc attggcgctc tcctgttcga cagcggcgag acggctgagg
     2521 ctacgcggct caagcgcacc gcccgcaggc ggtacacgcg caggaagaat cgcatctgct
     2581 acctgcagga gattttctcc aacgagatgg cgaaggttga cgattctttc ttccacaggc
     2641 tggaggagtc attcctcgtg gaggaggata agaagcacga gcggcatcca atcttcggca
     2701 acattgtcga cgaggttgcc taccacgaga agtaccctac gatctaccat ctgcggaaga
     2761 agctcgtgga ctccacagat aaggcggacc tccgcctgat ctacctcgct ctggcccaca
     2821 tgattaagtt caggggccat ttcctgatcg agggggatct caacccggac aatagcgatg
     2881 ttgacaagct gttcatccag ctcgtgcaga cgtacaacca gctcttcgag gagaacccca
     2941 ttaatgcgtc aggcgtcgac gcgaaggcta tcctgtccgc taggctctcg aagtctcggc
     3001 gcctcgagaa cctgatcgcc cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc
     3061 tcattgcgct cagcctgggg ctcacgccca acttcaagtc gaatttcgat ctcgctgagg
     3121 acgccaagct gcagctctcc aaggacacat acgacgatga cctggataac ctcctggccc
     3181 agatcggcga tcagtacgcg gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc
     3241 tcctgtctga tattctcagg gtgaacaccg agattacgaa ggctccgctc tcagcctcca
     3301 tgatcaagcg ctacgacgag caccatcagg atctgaccct cctgaaggcg ctggtcaggc
     3361 agcagctccc cgagaagtac aaggagatct tcttcgatca gtcgaagaac ggctacgctg
     3421 ggtacattga cggcggggcc tctcaggagg agttctacaa gttcatcaag ccgattctgg
     3481 agaagatgga cggcacggag gagctgctgg tgaagctcaa tcgcgaggac ctcctgagga
     3541 agcagcggac attcgataac ggcagcatcc cacaccagat tcatctcggg gagctgcacg
     3601 ctatcctgag gaggcaggag gacttctacc ctttcctcaa ggataaccgc gagaagatcg
     3661 agaagattct gactttcagg atcccgtact acgtcggccc actcgctagg ggcaactccc
     3721 gcttcgcttg gatgacccgc aagtcagagg agacgatcac gccgtggaac ttcgaggagg
     3781 tggtcgacaa gggcgctagc gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga
     3841 acctgccaaa tgagaaggtg ctccctaagc actcgctcct gtacgagtac ttcacagtct
     3901 acaacgagct gactaaggtg aagtatgtga ccgagggcat gaggaagccg gctttcctgt
     3961 ctggggagca gaagaaggcc atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg
     4021 ttaagcagct caaggaggac tacttcaaga agattgagtg cttcgattcg gtcgagatct
     4081 ctggcgttga ggaccgcttc aacgcctccc tggggaccta ccacgatctc ctgaagatca
     4141 ttaaggataa ggacttcctg gacaacgagg agaatgagga tatcctcgag gacattgtgc
     4201 tgacactcac tctgttcgag gaccgggaga tgatcgagga gcgcctgaag acttacgccc
     4261 atctcttcga tgacaaggtc atgaagcagc tcaagaggag gaggtacacc ggctggggga
     4321 ggctgagcag gaagctcatc aacggcattc gggacaagca gtccgggaag acgatcctcg
     4381 acttcctgaa gagcgatggc ttcgcgaacc gcaatttcat gcagctgatt cacgatgaca
     4441 gcctcacatt caaggaggat atccagaagg ctcaggtgag cggccagggg gactcgctgc
     4501 acgagcatat cgcgaacctc gctggctcgc cagctatcaa gaaggggatt ctgcagaccg
     4561 tgaaggttgt ggacgagctg gtgaaggtca tgggcaggca caagcctgag aacatcgtca
     4621 ttgagatggc ccgggagaat cagaccacgc agaagggcca gaagaactca cgcgagagga
     4681 tgaagaggat cgaggagggc attaaggagc tggggtccca gatcctcaag gagcacccgg
     4741 tggagaacac gcagctgcag aatgagaagc tctacctgta ctacctccag aatggccgcg
     4801 atatgtatgt ggaccaggag ctggatatta acaggctcag cgattacgac gtcgatcata
     4861 tcgttccaca gtcattcctg aaggatgact ccattgacaa caaggtcctc accaggtcgg
     4921 acaagaaccg gggcaagtct gataatgttc cttcagagga ggtcgttaag aagatgaaga
     4981 actactggcg ccagctcctg aatgccaagc tgatcacgca gcggaagttc gataacctca
     5041 caaaggctga gaggggcggg ctctctgagc tggacaaggc gggcttcatc aagaggcagc
     5101 tggtcgagac acggcagatc actaagcacg ttgcgcagat tctcgactca cggatgaaca
     5161 ctaagtacga tgagaatgac aagctgatcc gcgaggtgaa ggtcatcacc ctgaagtcaa
     5221 agctcgtctc cgacttcagg aaggatttcc agttctacaa ggttcgggag atcaacaatt
     5281 accaccatgc ccatgacgcg tacctgaacg cggtggtcgg cacagctctg atcaagaagt
     5341 acccaaagct cgagagcgag ttcgtgtacg gggactacaa ggtttacgat gtgaggaaga
     5401 tgatcgccaa gtcggagcag gagattggca aggctaccgc caagtacttc ttctactcta
     5461 acattatgaa tttcttcaag acagagatca ctctggccaa tggcgagatc cggaagcgcc
     5521 ccctcatcga gacgaacggc gagacggggg agatcgtgtg ggacaagggc agggatttcg
     5581 cgaccgtcag gaaggttctc tccatgccac aagtgaatat cgtcaagaag acagaggtcc
     5641 agactggcgg gttctctaag gagtcaattc tgcctaagcg gaacagcgac aagctcatcg
     5701 cccgcaagaa ggactgggat ccgaagaagt acggcgggtt cgacagcccc actgtggcct
     5761 actcggtcct ggttgtggcg aaggttgaga agggcaagtc caagaagctc aagagcgtga
     5821 aggagctgct ggggatcacg attatggagc gctccagctt cgagaagaac ccgatcgatt
     5881 tcctggaggc gaagggctac aaggaggtga agaaggacct gatcattaag ctccccaagt
     5941 actcactctt cgagctggag aacggcagga agcggatgct ggcttccgct ggcgagctgc
     6001 agaaggggaa cgagctggct ctgccgtcca agtatgtgaa cttcctctac ctggcctccc
     6061 actacgagaa gctcaagggc agccccgagg acaacgagca gaagcagctg ttcgtcgagc
     6121 agcacaagca ttacctcgac gagatcattg agcagatttc cgagttctcc aagcgcgtga
     6181 tcctggccga cgcgaatctg gataaggtcc tctccgcgta caacaagcac cgcgacaagc
     6241 caatcaggga gcaggctgag aatatcattc atctcttcac cctgacgaac ctcggcgccc
     6301 ctgctgcttt caagtacttc gacacaacta tcgatcgcaa gaggtacaca agcactaagg
     6361 aggtcctgga cgcgaccctc atccaccagt cgattaccgg cctctacgag acgcgcatcg
     6421 acctgtctca gctcgggggc gacaagcggc cagcggcgac gaagaaggcg gggcaggcga
     6481 agaagaagaa gtgagctcag agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa
     6541 gttcaatgca tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg
     6601 cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta
     6661 ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata
     6721 tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt
     6781 tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc
     6841 gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg
     6901 cttattcact aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat
     6961 acaagtatgt cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga
     7021 atccttgtca gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt
     7081 gagtatgaaa atatttttta atgcatttta tgacttgcca attgttccgg aactctagat
     7141 aagcttaccg gaaagggcga attcgcaact ttgtatacaa aagttgcccc atggcgttcc
     7201 ctctagataa cgcaggatcc tatttacttt aaatttttct tatggctcag cctgtgatgg
     7261 ataactgaat caaacaaatg gcgtctgggt ttaagaacat ctgttttggc tatgttggac
     7321 gaaacaagtg aacttttagg atcaacttcc gtttatatac ggagcttata tcgagcaata
     7381 agataagtgg gctttttatg taatttaatg ggctatcgtc catatattca ctaataccca
     7441 tgcccagtac ccatgtatgc gtttcatata agctcctaat ttctcccaca tcgctcaaat
     7501 ctaaacaaat cttgttgtat atataacact gagggagcac cattggtcag agaccggcgc
     7561 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     7621 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     7681 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     7741 cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc
     7801 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     7861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7981 cccgctttcc accggtggtc tctgttttag agctagaaat agcaagttaa aataaggcta
     8041 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttttccc tttccttttt
     8101 tctttttttt gccataaact taaatttgta tatcgatcat tgtagatatt gaaaacctag
     8161 aacaaaccaa catccatgtg aatgtctttc atgactgatt tagagataat tcttgaattt
     8221 tggaactaga atctataatg agcctaaatt aaaacattgt gaactagtaa gggcgaattc
     8281 gacccagctt tcttgtacaa agtggttcga taattcttaa ttaactagtt ctagagcggc
     8341 cgccaccgcg gtggagctcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     8401 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8641 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct
     8701 tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
     8761 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
     8821 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
     8881 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
     8941 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
     9001 cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact
     9061 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     9121 gggtaatatc gggaaacctc ctcggattcc attgccnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
     9301 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     9361 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     9421 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     9481 tgaaatcacc agtctctctc tacaaatcta tctctctcga gtctaccatg agcccagaac
     9541 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     9601 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     9661 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
     9721 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
     9781 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
     9841 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
     9901 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
     9961 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
    10021 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatttgac
    10081 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
    10141 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
    10201 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
    10261 ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
    10321 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    10381 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    10441 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    10501 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    10561 gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct
    10621 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
    10681 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
    10741 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
    10801 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
    10861 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
    10921 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
    10981 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
    11041 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
    11101 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
    11161 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
    11221 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
    11281 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
    11341 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
    11401 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
    11461 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
    11521 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
    11581 gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11701 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    11821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    12001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    12061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    12121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    12181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    12301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    12361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    12421 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
    12481 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    12541 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
    12601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    12661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
    12721 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
    12781 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
    12841 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
    12901 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
    12961 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
    13021 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
    13081 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaacagac cttttcgacc
    13141 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
    13201 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
    13261 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
    13321 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
    13381 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
    13441 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
    13501 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
    13561 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
    13621 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
    13681 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
    13741 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
    13801 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
    13861 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
    13921 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
    13981 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    14041 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    14101 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    14161 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    14221 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    14281 gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc
    14341 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    14401 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    14461 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    14521 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    14581 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    14641 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    14701 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    14761 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    14821 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    14881 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    14941 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    15001 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    15061 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    15121 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    15181 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    15241 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    15301 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    15361 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    15421 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    15481 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    15541 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    15601 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    15661 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    15721 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    15781 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    15841 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    15901 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    15961 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    16021 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    16081 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    16141 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    16201 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    16261 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    16321 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    16381 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    16441 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    16501 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    16561 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    16621 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    16681 ctgatccaag ctcaagctgc tctagcattc gccattcagg ctgcgcaact gttgggaagg
    16741 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    16801 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    16861 tg
//
