LOCUS       sequence-45231-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CeGFP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        41..59
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     41..58
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             145..858
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(175..203)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     798..818
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa
       61 tacaagctac ttgttctttt tgcaggatcc actagtggcg cgccattaat taaaggccgg
      121 ccagcgatcg ccggacatcg attgatgagt aaaggagaag aacttttcac tggagttgtc
      181 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      241 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      301 ctacctgttc catggccaac acttgtcact actctcactt atggtgttca atgcttttca
      361 agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
      421 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      481 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      541 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      601 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      661 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      721 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      781 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      841 atggatgaac tatacaaata agaattc
//
