LOCUS       sequence-452316-       12913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate level 1, position 2, ePEmax3 expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY
  TITLE     Optimized dicot prime editing enables heritable desired edits in 
            tomato and Arabidopsis.
  JOURNAL   Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w.
  PUBMED    39242983
REFERENCE   2  (bases 1 to 12913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2024 Sep 6. doi: 10.1038/s41477-024-01786-w."
COMMENT     Sequence Label: pICH47742::p35S-ePEmax3::tEURb7
FEATURES             Location/Qualifiers
     source          1..12913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1023..1368
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1311..1331
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1495..1515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1516..5616
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5842..5862
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7558..7578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7588..7614
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    8616..8640
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(8796..9944)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(10280..10868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10360..10379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11039..11830,11831..11899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11662..11681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11900..12004)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12091..12722
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    12814..12838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggaattcc aatcccacaa aaatctgagc
       61 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
      121 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
      181 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
      241 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
      301 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
      361 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
      421 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
      481 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
      541 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
      601 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
      661 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
      721 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
      781 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
      841 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
      901 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
      961 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     1021 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     1081 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     1141 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     1201 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     1261 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     1321 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     1381 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     1441 tttacaatta tcgatacaat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag
     1501 aagaagcgga aagtcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg
     1561 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1621 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1681 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     1741 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     1801 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     1861 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     1921 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     1981 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     2041 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     2101 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     2161 agcaagagca gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     2221 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     2281 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     2341 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2401 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2461 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2521 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2581 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2641 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag
     2701 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     2761 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     2821 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     2881 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     2941 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     3001 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     3061 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     3121 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     3181 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     3241 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     3301 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3361 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3421 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3481 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3541 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3601 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3661 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     3721 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     3781 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     3841 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     3901 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     3961 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     4021 gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     4081 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     4141 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     4201 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     4261 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     4321 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4381 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4441 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4501 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4561 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4621 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4681 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     4741 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     4801 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     4861 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     4921 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     4981 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     5041 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     5101 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     5161 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     5221 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     5281 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     5341 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5401 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5461 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5521 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5581 gagacacgga tcgacctgtc tcagctggga ggtgactccg gcggaagctc tggtggcagc
     5641 aagcggaccg ccgacggctc tgaattcgag agcgccacag tggtgtccgg ccaaaagcag
     5701 gaccgccagg gcggagaacg cagaaggtcc cagctcgata gggatcagtg tgcctactgc
     5761 aaggagaagg gccactgggc caaagactgc ccgaaaaagc cgcgcggccc acgcggccca
     5821 aggccacaaa catccctcct tccaaagaag aagcggaagg tggagctcag cggaggatct
     5881 tccggaggat ctagcggctc cgagacacca ggaacatccg aaagcgctac accagaatct
     5941 agcggaggct cttccggagg atctaggcct accctgaaca ttgaagatga gtatagactg
     6001 catgaaacaa gcaaggaacc cgacgtgtcc ctgggctcca cctggctgtc cgactttccc
     6061 caggcctggg ccgagacagg aggaatgggc ctggccgtgc ggcaggcacc cctgatcatc
     6121 cctctgaagg ccacctctac acccgtgagc atcaagcagt accctatgtc tcaggaggcc
     6181 agactgggca tcaagcctca catccagagg ctgctggacc agggcatcct ggtgccatgc
     6241 cagagcccct ggaacacacc actgctgccc gtgaagaagc caggcaccaa tgactataga
     6301 cccgtgcagg atctgagaga ggtgaacaag agggtggagg atatccaccc caccgtgccc
     6361 aacccttaca atctgctgtc cggcctgccc ccttctcacc agtggtatac agtgctggac
     6421 ctgaaggatg ccttcttttg tctgagactg caccctacca gccagccact gttcgccttt
     6481 gagtggaggg accctgagat gggcatctct ggccagctga cctggacacg cctgcctcag
     6541 ggcttcaaga atagcccaac actgtttaac gaggccctgc accgcgacct ggcagatttc
     6601 cggatccagc acccagatct gatcctgctg cagtacgtgg acgatctgct gctggccgcc
     6661 accagcgagc tggattgcca gcagggaaca cgcgccctgc tgcagaccct gggaaacctg
     6721 ggatataggg catccgccaa gaaggcccag atctgtcaga agcaggtgaa gtacctgggc
     6781 tatctgctga aggagggcca gagatggctg acagaggcca ggaaggagac agtgatgggc
     6841 cagccaacac ccaagacccc aagacagctg agggagttcc tgggcaaagc aggattttgc
     6901 aggctgttca tcccaggatt cgcagagatg gcagcacctc tgtacccact gaccaagccg
     6961 ggcaccctgt ttaattgggg ccctgaccag cagaaggcct atcaggagat caagcaggcc
     7021 ctgctgacag caccagccct gggcctgcca gacctgacca agcctttcga gctgtttgtg
     7081 gatgagaagc agggctacgc caagggcgtg ctgacccaga agctgggacc atggagacgg
     7141 cccgtggcct atctgtccaa gaagctggac ccagtggcag caggatggcc accatgcctg
     7201 aggatggtgg cagcaatcgc cgtgctgaca aaggatgccg gcaagctgac catgggacag
     7261 ccactggtca tcctggcacc acacgcagtg gaggccctgg tgaagcagcc tccagatcgc
     7321 tggctgtcta acgcccggat gacacactac caggccctgc tgctggacac cgatcgcgtg
     7381 cagtttggcc ctgtggtggc cctgaatcca gccaccctgc tgcctctgcc agaggagggc
     7441 ctgcagcaca actgtctgga catcctggca gaggcacacg gaacaaggcc agacctgacc
     7501 gatcagcccc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagtctccc
     7561 aagaagaaga ggaaagtcgg ctctggccct gccgctaaga gagtgaagct ggactaagct
     7621 taaagcagaa tgctgagcta aaagaaaggc tttttccatt ttcgagagac aatgagaaaa
     7681 gaagaagaag aagaagaaga agaagaagaa gaagaagaaa agagtaaata ataaagcccc
     7741 acaggaggcg aagttcttgt agctccatgt tatctaagtt attgatattg tttgccctat
     7801 attttatttc tgtcattgtg tatgttttgt tcagtttcga tctccttgca aaatgcagag
     7861 attatgagat gaataaacta agttatatta ttatacgtgt taatattctc ctcctctctc
     7921 tagctagcct tttgttttct ctttttctta tttgattttc tttaaatcaa tccattttag
     7981 gagagggcca gggagtgatc cagcaaaaca tgaagattag aagaaacttc cctctttttt
     8041 ttcctgaaaa caatttaacg tcgagattta tctctttttg taatggaatc atttctacag
     8101 ttatgacacc aactcggtcc atttgcaccc ctaatcataa tagctttaat atttcaagat
     8161 attattaagt taacgttgtc aatatcctgg aaattttgca aaatgaatca agcctatatg
     8221 gctgtaatat gaatttaaaa gcagctcgat gtggtggtaa tatgtaattt acttgattct
     8281 aaaaaaatat cccaagtatt aataatttct gctaggaaga aggttagcta cgatttacag
     8341 caaagccaga atacaaagaa ccataaagtg attgaagctc gaaatatacg aaggaacaaa
     8401 tatttttaaa aaaatacgca atgacttgga acaaaagaaa gtgatatatt ttttgttctt
     8461 aaacaagcat cccctctaaa gaatggcagt tttcctttgc atgtaactat tatgctccct
     8521 tcgttacaaa aattttggac tactattggg aacttcttct gaaaatagtg cgctactatt
     8581 gtcttctgca cgaagtggtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     8641 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     8701 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt ccccagatca ggcgctggct
     8761 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
     8821 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
     8881 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
     8941 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
     9001 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
     9061 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
     9121 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
     9181 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
     9241 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
     9301 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
     9361 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
     9421 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
     9481 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
     9541 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
     9601 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
     9661 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcaag acgacgcgaa
     9721 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
     9781 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
     9841 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
     9901 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
     9961 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
    10021 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
    10081 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
    10141 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
    10201 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg atctgccagg aaccgtaaaa
    10261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10981 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11041 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11101 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11161 gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca gcaataaacc
    11221 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11281 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11341 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11401 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11461 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11521 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11581 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11641 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11701 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11761 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11821 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11881 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcaaggtt
    11941 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12001 cgcgcacgaa ttggccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg
    12061 cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg
    12121 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
    12181 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
    12241 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
    12301 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
    12361 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
    12421 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
    12481 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
    12541 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
    12601 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
    12661 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
    12721 tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg attttgtgcc
    12781 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
    12841 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact ggggtggatg
    12901 cagtgggccc cac
//
