LOCUS       sequence-452319-        8211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RIG-I-Myc expression vector for overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Takahashi T, Nakano Y, Onomoto K, Yoneyama M, Ui-Tei K
  TITLE     Virus Sensor RIG-I Represses RNA Interference by Interacting with 
            TRBP through LGP2 in Mammalian Cells.
  JOURNAL   Genes (Basel). 2018 Oct 19;9(10):511. doi: 10.3390/genes9100511.
  PUBMED    30347765
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2018-10-19"; volume: "9(10):511. doi"; pages: " 
            10.3390/genes9100511"
COMMENT     Sequence Label: pEF-BOS-RIG-I-Myc
FEATURES             Location/Qualifiers
     source          1..8211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2254..2464
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2276..2472
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2323..2458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2559..3737
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2790..3728
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3685..3705
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6549..6560
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6570..6599
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6600..6617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7341..7358)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7341..7357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7350..7372)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7570..8025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7657..7676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7867..7888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8042..8061)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8161..8183
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt aattccctcc ccagcaggca gaagtatgca aagcatgcat
     2281 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2341 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2401 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2461 ggcttttgca aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca
     2521 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2581 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2641 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2701 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2761 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2821 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2881 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2941 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     3001 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     3061 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     3121 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3181 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3241 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg tcgtgtatcg
     3301 ccccgccctg ggcggcaagg ctggtctggt cggcaccagt tgcgtgagcg gaaagatggc
     3361 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3421 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3481 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgtgc ttttggagta
     3541 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3601 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3661 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3721 catttcaggt gtcgtgagga attctctaga gatccctcga cctcgagatg accaccgagc
     3781 agcgacgcag cctgcaagcc ttccaggatt atatccggaa gaccctggac cctacctaca
     3841 tcctgagcta catggccccc tggtttaggg aggaagaggt gcagtatatt caggctgaga
     3901 aaaacaacaa gggcccaatg gaggctgcca cactttttct caagttcctg ttggagctcc
     3961 aggaggaagg ctggttccgt ggctttttgg atgccctaga ccatgcaggt tattctggac
     4021 tttatgaagc cattgaaagt tgggatttca aaaaaattga aaagttggag gagtatagat
     4081 tacttttaaa acgtttacaa ccagaattta aaaccagaat tatcccaacc gatatcattt
     4141 ctgatctgtc tgaatgttta attaatcagg aatgtgaaga aattctacag atttgctcta
     4201 ctaaggggat gatggcaggt gcagagaaat tggtggaatg ccttctcaga tcagacaagg
     4261 aaaactggcc caaaactttg aaacttgctt tggagaaaga aaggaacaag ttcagtgaac
     4321 tgtggattgt agagaaaggt ataaaagatg ttgaaacaga agatcttgag gataagatgg
     4381 aaacttctga catacagatt ttctaccaag aagatccaga atgccagaat cttagtgaga
     4441 attcatgtcc accttcagaa gtgtctgata caaacttgta cagcccattt aaaccaagaa
     4501 attaccaatt agagcttgct ttgcctgcta tgaaaggaaa aaacacaata atatgtgctc
     4561 ctacaggttg tggaaaaacc tttgtttcac tgcttatatg tgaacatcat cttaaaaaat
     4621 tcccacaagg acaaaagggg aaagttgtct tttttgcgaa tcagatccca gtgtatgaac
     4681 agcagaaatc tgtattctca aaatactttg aaagacatgg gtatagagtt acaggcattt
     4741 ctggagcaac agctgagaat gtcccagtgg aacagattgt tgagaacaat gacatcatca
     4801 ttttaactcc acagattctt gtgaacaacc ttaaaaaggg aacgattcca tcactatcca
     4861 tctttacttt gatgatattt gatgaatgcc acaacactag taaacaacac ccgtacaata
     4921 tgatcatgtt taattatcta gatcagaaac ttggaggatc ttcaggccca ctgccccagg
     4981 tcattgggct gactgcctcg gttggtgttg gggatgccaa aaacacagat gaagccttgg
     5041 attatatctg caagctgtgt gcttctcttg atgcgtcagt gatagcaaca gtcaaacaca
     5101 atctggagga actggagcaa gttgtttata agccccagaa gtttttcagg aaagtggaat
     5161 cacggattag cgacaaattt aaatacatca tagctcagct gatgagggac acagagagtc
     5221 tggcaaagag aatctgcaaa gacctcgaaa acttatctca aattcaaaat agggaatttg
     5281 gaacacagaa atatgaacaa tggattgtta cagttcagaa agcatgcatg gtgttccaga
     5341 tgccagacaa agatgaagag agcaggattt gtaaagccct gtttttatac acttcacatt
     5401 tgcggaaata taatgatgcc ctcattatca gtgagcatgc acgaatgaaa gatgctctgg
     5461 attacttgaa agacttcttc agcaatgtcc gagcagcagg attcgatgag attgagcaag
     5521 atcttactca gagatttgaa gaaaagctgc aggaactaga aagtgtttcc agggatccca
     5581 gcaatgagaa tcctaaactt gaagacctct gcttcatctt acaagaagag taccacttaa
     5641 acccagagac aataacaatt ctctttgtga aaaccagagc acttgtggac gctttaaaaa
     5701 attggattga aggaaatcct aaactcagtt ttctaaaacc tggcatattg actggacgtg
     5761 gcaaaacaaa tcagaacaca ggaatgaccc tcccggcaca gaagtgtata ttggatgcat
     5821 tcaaagccag tggagatcac aatattctga ttgccacctc agttgctgat gaaggcattg
     5881 acattgcaca gtgcaatctt gtcatccttt atgagtatgt gggcaatgtc atcaaaatga
     5941 tccaaaccag aggcagagga agagcaagag gtagcaagtg cttccttctg actagtaatg
     6001 ctggtgtaat tgaaaaagaa caaataaaca tgtacaaaga aaaaatgatg aatgactcta
     6061 ttttacgcct tcagacatgg gacgaagcag tatttaggga aaagattctg catatacaga
     6121 ctcatgaaaa attcatcaga gatagtcaag aaaaaccaaa acctgtacct gataaggaaa
     6181 ataaaaaact gctctgcaga aagtgcaaag ccttggcatg ttacacagct gacgtaagag
     6241 tgatagagga atgccattac actgtgcttg gagatgcttt taaggaatgc tttgtgagta
     6301 gaccacatcc caagccaaag cagttttcaa gttttgaaaa aagagcaaag atattctgtg
     6361 cccgacagaa ctgcagccat gactggggaa tccatgtgaa gtacaagaca tttgagattc
     6421 cagttataaa aattgaaagt tttgtggtgg aggatattgc aactggagtt cagacgctgt
     6481 actcgaagtg gaaggacttt cattttgaga agataccatt tgatccagca gaaatgtcca
     6541 aaggatccat tgaaggaagg atcgtcaagg aacaaaaact catctcagaa gaggatctgc
     6601 accaccacca ccaccactga gcggccgcga ctctagagtg agggtcccca cctgggaccc
     6661 ttgagagtat caggtctccc acgtgggaga caagaaatcc ctgtttaata tttaaacagc
     6721 agtgttcccc atctgggtcc ttgcacccct cactctggcc tcagccgact gcacagcggc
     6781 ccctgcatcc ccttggctgt gaggcccctg gacaagcaga ggtggccaga gctgggaggc
     6841 atggccctgg ggtcccacga atttgctggg gaatctcgtt tttcttctta agacttttgg
     6901 gacatggttt gactcccgaa catcaccgac gtgtctcctg tttttctggg tggcctcggg
     6961 acacctgccc tgcccccacg agggtcagga ctgtgactct ttttagggcc aggcaggtgc
     7021 ctggacattt gccttgctgg acggggactg gggatgtggg agggagcaga caggaggaat
     7081 catgtcaggc ctgtgtgtga aaggaagctc cactgtcacc ctccacctct tcacccccca
     7141 ctcaccagtg tcccctccac tgtcacattg taactgaact tcaggataat aaagtgtttg
     7201 cctccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7321 aaaaaaaaaa aaccgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7381 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7441 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     7501 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     7561 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7621 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     7681 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7741 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     7801 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7861 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     7921 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     7981 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     8041 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     8101 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8161 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
