LOCUS       sequence-452321-       10405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell 
            JE Jr, Boettiger AN, Wysocka J
  TITLE     Long-range regulation of transcription scales with genomic distance 
            in a gene-specific manner.
  JOURNAL   Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 
            10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2.
  PUBMED    39626660
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.10.021"; journalName: "Mol Cell"; date: 
            "2025-01-16- 16"; volume: "85"; issue: "2"; pages: "347-361.e7"
COMMENT     Sequence Label: pk335-CARGO-11mer-35kb-DSF-1-11
FEATURES             Location/Qualifiers
     source          1..10405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5973..6258
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        6260..6539
                     /label=chicken beta-actin promoter
     intron          6540..6767
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             6785..7486
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYDDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(7511..7528)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7517..7724
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7728..7797
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   7800..7940
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7800..7929
                     /label=AAV2 ITR
     rep_origin      8015..8470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8102..8121)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8312..8333
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8487..8506)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8535..8558)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8932..8951
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9021..9068
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        9060..9078
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9082..9091
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9088..9891
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9151..9170)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9761..9780
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gtttaagagc
      661 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
      721 ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcttt cccatgattc
      781 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
      841 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
      901 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
      961 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgct gcagatacct
     1021 gcagagttgt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     1081 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tctcgagtac taggatccat
     1141 taggctttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     1201 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     1261 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     1321 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1381 acaccgctta atcatgtctg tttaccgttt aagagctatg ctggaaacag catagcaagt
     1441 ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
     1501 tcgagtacta ggatccatta ggctttccca tgattccttc atatttgcat atacgataca
     1561 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     1621 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     1681 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgaaac accgtaaaat aacagactac tccggtttaa gagctatgct
     1801 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     1861 gagtcggtgc tttttttctc gagtactagg atccattagg ctttcccatg attccttcat
     1921 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1981 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2041 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2101 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtttatatt cattcaatta
     2161 gcgtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     2221 acttgaaaaa gtggcaccga gtcggtgctt tttttctcga gtactaggat ccattaggct
     2281 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     2341 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     2401 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     2461 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     2521 tatttcctcg tcccctctgc gtttaagagc tatgctggaa acagcatagc aagtttaaat
     2581 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttctcgagt
     2641 actaggatcc attaggcttt cccatgattc cttcatattt gcatatacga tacaaggctg
     2701 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2761 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2821 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2881 ggaaaggacg aaacaccgtg gtgcacatta attagtaggt ttaagagcta tgctggaaac
     2941 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     3001 gtgctttttt tctcgagtac taggatccat taggctttcc catgattcct tcatatttgc
     3061 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     3121 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     3181 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     3241 tggctttata tatcttgtgg aaaggacgaa acaccgtttt ttttctaggt actaggatcc
     3301 attaggcgta ctaggatcca ttcatgtgtt taagagctat gctggaaaca gcatagcaag
     3361 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     3421 ctcgagtact aggatccatt aggctttccc atgattcctt catatttgca tatacgatac
     3481 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     3541 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     3601 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     3661 atcttgtgga aaggacgaaa caccgaaagt ctagacatct tgttggttta agagctatgc
     3721 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     3781 cgagtcggtg ctttttttct cgagtactag gatccattag gctttcccat gattccttca
     3841 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     3901 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     3961 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     4021 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgaactctc tagaagtttg
     4081 agcgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     4141 aacttgaaaa agtggcaccg agtcggtgct ttttttctcg agtactagga tccattaggc
     4201 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     4261 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     4321 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     4381 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     4441 gtggcttgga ggataactaa tgtttaagag ctatgctgga aacagcatag caagtttaaa
     4501 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag
     4561 tactaggatc cattaggctt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     4621 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     4681 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     4741 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     4801 tggaaaggac gaaacaccga tggaaaggtc agtgtgacag tttaagagct atgctggaaa
     4861 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4921 ggtgcttttt ttctcgagta ctaggatcca ttaggctttc ccatgattcc ttcatatttg
     4981 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     5041 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     5101 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     5161 ttggctttat atatcttgtg gaaaggacga aacaccgtca cgtggtgcat acgactggtt
     5221 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     5281 aaaaagtggc accgagtcgg tgcttttttt ctcgagtact aggatccatt aggctttccc
     5341 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     5401 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     5461 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     5521 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgctgca
     5581 gatacctgca gagttgttta agagctatgc tggaaacagc atagcaagtt taaataaggc
     5641 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag
     5701 gatccattag gctttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     5761 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     5821 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     5881 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     5941 ggacgaaaca ccgttttttt tctagaggta cccgttacat aacttacggt aaatggcccg
     6001 cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact
     6061 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6121 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6181 cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6241 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     6301 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     6361 ggggcggggg gggggggggg gggcgcgcgc sagggggggg gggggggggs gaggggsggg
     6421 gsggggsgag gsggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     6481 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     6541 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     6601 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     6661 cgggctgtaa ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat
     6721 taatgtttaa ttacctggag cacctgcctg aaatcacttt ttttcaggtt ggaccggtgc
     6781 caccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     6841 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     6901 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     6961 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     7021 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgatgacgg
     7081 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     7141 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     7201 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     7261 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag
     7321 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     7381 ggaaagaatc aaggaggcca acaacgaaac ctacgtcgag cagcacgagg tggcagtggc
     7441 cagatactgc gacctcccta gcaaactggg gcacaagctt aattaagaat tcctagagct
     7501 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7561 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7621 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7681 agcaaggggg aggattggga agagaatagc aggcatgctg gggagctctc gaagccgcgg
     7741 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatcgca
     7801 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     7861 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     7921 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7981 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     8041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     8101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8221 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8281 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8341 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     8401 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8461 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gtgcttttcc
     8521 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     8581 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     8641 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     8701 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     8761 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     8821 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     8881 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     8941 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     9001 ccgatcatca agctgcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     9061 aatacgactc actataggag ggccaccatg ggatcggcca ttgaacaaga tggattgcac
     9121 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     9181 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     9241 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     9301 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9361 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     9421 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9481 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9541 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9601 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     9661 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9721 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     9781 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     9841 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg atcgagaaat
     9901 tgatgatcta ttaaacaata aagacgtcca catggaagtg caaactatta actggcgaac
     9961 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10021 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10081 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10141 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10201 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10261 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10321 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10381 ccgtagaaaa gatcaaagga tcttc
//
