LOCUS       sequence-452325-       10404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell 
            JE Jr, Boettiger AN, Wysocka J
  TITLE     Long-range regulation of transcription scales with genomic distance 
            in a gene-specific manner.
  JOURNAL   Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 
            10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2.
  PUBMED    39626660
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.10.021"; journalName: "Mol Cell"; date: 
            "2025-01-16- 16"; volume: "85"; issue: "2"; pages: "347-361.e7"
COMMENT     Sequence Label: pk335-CARGO-12mer-14kb-USF-1-12
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5973..6258
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        6260..6538
                     /label=chicken beta-actin promoter
     intron          6539..6766
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             6784..7485
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYDDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(7510..7527)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7516..7723
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7727..7796
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   7799..7939
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7799..7928
                     /label=AAV2 ITR
     rep_origin      8014..8469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8101..8120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8311..8332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8486..8505)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8534..8557)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8931..8950
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9020..9067
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        9059..9077
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9081..9090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9087..9890
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9150..9169)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9760..9779
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gtttaagagc
      661 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
      721 ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcttt cccatgattc
      781 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
      841 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
      901 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
      961 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgag gtttaaaaga
     1021 agagtatagt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     1081 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tctcgagtac taggatccat
     1141 taggctttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     1201 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     1261 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     1321 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1381 acaccggcac atggtaaagc gctagggttt aagagctatg ctggaaacag catagcaagt
     1441 ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
     1501 tcgagtacta ggatccatta ggctttccca tgattccttc atatttgcat atacgataca
     1561 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     1621 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     1681 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgaaac accgcagaaa ttagcccatg ttccgtttaa gagctatgct
     1801 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     1861 gagtcggtgc tttttttctc gagtactagg atccattagg ctttcccatg attccttcat
     1921 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1981 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2041 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2101 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgatggtacc aagggcgagt
     2161 cagtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     2221 acttgaaaaa gtggcaccga gtcggtgctt tttttctcga gtactaggat ccattaggct
     2281 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     2341 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     2401 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     2461 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     2521 atgaagtcgt acctctaact gtttaagagc tatgctggaa acagcatagc aagtttaaat
     2581 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttctcgagt
     2641 actaggatcc attaggcttt cccatgattc cttcatattt gcatatacga tacaaggctg
     2701 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     2761 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2821 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2881 ggaaaggacg aaacaccgaa tcttagcatg aactgggggt ttaagagcta tgctggaaac
     2941 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     3001 gtgctttttt tctcgagtac taggatccat taggctttcc catgattcct tcatatttgc
     3061 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     3121 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     3181 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     3241 tggctttata tatcttgtgg aaaggacgaa acaccgtttt ttttctaggt actaggatcc
     3301 attaggcgta ctaggatcca ttcatgtgtt taagagctat gctggaaaca gcatagcaag
     3361 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     3421 ctcgagtact aggatccatt aggctttccc atgattcctt catatttgca tatacgatac
     3481 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     3541 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     3601 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     3661 atcttgtgga aaggacgaaa caccgtgcac acctgttccc atatagttta agagctatgc
     3721 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     3781 cgagtcggtg ctttttttct cgagtactag gatccattag gctttcccat gattccttca
     3841 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     3901 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     3961 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     4021 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggacagga gcactcaagt
     4081 gcggtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     4141 aacttgaaaa agtggcaccg agtcggtgct ttttttctcg agtactagga tccattaggc
     4201 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     4261 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     4321 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     4381 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     4441 gtgcaacagg ataggccata agtttaagag ctatgctgga aacagcatag caagtttaaa
     4501 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag
     4561 tactaggatc cattaggctt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     4621 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     4681 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     4741 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     4801 tggaaaggac gaaacaccgc tttgtaaaca tctgagtctg tttaagagct atgctggaaa
     4861 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4921 ggtgcttttt ttctcgagta ctaggatcca ttaggctttc ccatgattcc ttcatatttg
     4981 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     5041 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     5101 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     5161 ttggctttat atatcttgtg gaaaggacga aacaccgaaa tttgagggac gtggatggtt
     5221 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     5281 aaaaagtggc accgagtcgg tgcttttttt ctcgagtact aggatccatt aggctttccc
     5341 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     5401 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     5461 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     5521 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggggac
     5581 ggaaagtaca atgaggttta agagctatgc tggaaacagc atagcaagtt taaataaggc
     5641 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag
     5701 gatccattag gctttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     5761 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     5821 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     5881 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     5941 ggacgaaaca ccgttttttt tctagaggta cccgttacat aacttacggt aaatggcccg
     6001 cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact
     6061 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6121 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6181 cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6241 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     6301 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     6361 ggggcggggg gggggggggg ggcgcgcgcs aggsggggsg gggsggggsg aggggsgggg
     6421 sggggsgagg sggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     6481 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     6541 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     6601 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     6661 gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt
     6721 aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc
     6781 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     6841 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     6901 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     6961 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     7021 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgatgacggg
     7081 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     7141 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     7201 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     7261 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
     7321 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     7381 gaaagaatca aggaggccaa caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc
     7441 agatactgcg acctccctag caaactgggg cacaagctta attaagaatt cctagagctc
     7501 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7561 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7621 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7681 gcaaggggga ggattgggaa gagaatagca ggcatgctgg ggagctctcg aagccgcggt
     7741 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatcgcag
     7801 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     7861 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     7921 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     7981 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     8041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     8101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8221 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8341 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     8401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     8461 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag tgcttttccc
     8521 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     8581 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     8641 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     8701 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     8761 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     8821 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     8881 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     8941 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     9001 cgatcatcaa gctgcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     9061 atacgactca ctataggagg gccaccatgg gatcggccat tgaacaagat ggattgcacg
     9121 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     9181 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     9241 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     9301 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     9361 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     9421 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     9481 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     9541 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     9601 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     9661 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     9721 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     9781 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     9841 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga tcgagaaatt
     9901 gatgatctat taaacaataa agacgtccac atggaagtgc aaactattaa ctggcgaact
     9961 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10021 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10081 tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10141 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10201 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10261 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10321 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10381 cgtagaaaag atcaaaggat cttc
//
