LOCUS       sequence-452332-        2493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response 
            element from the human NANOG promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Tang B, Raviv A, Esposito D, Flanders KC, Daniel C, Nghiem BT, 
            Garfield S, Lim L, Mannan P, Robles AI, Smith WI Jr, Zimmerberg J, 
            Ravin R, Wakefield LM
  TITLE     A flexible reporter system for direct observation and isolation of 
            cancer stem cells.
  JOURNAL   Stem Cell Reports. 2015 Jan 13;4(1):155-169. doi: 
            10.1016/j.stemcr.2014.11.002. Epub 2014 Dec 11.
  PUBMED    25497455
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.11.002"; journalName: "Stem Cell Reports"; 
            date: "2015-01-13- 13"; volume: "4"; issue: "1"; pages: "155-169"
COMMENT     Sequence Label: 8138-E02
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..97
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(342..437)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(556..575)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             567..1376
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(634..653)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1519..2107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2008..2027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2242..2269)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2361..2447)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttggcc aaattacaaa attcaaaatt
      121 ttatcgatca gctacttttg cattacaatg gccttggtgc agctactttt gcattacaat
      181 ggccttggtg cagctacttt tgcattacaa tggccttggt ggaattccag ctacttttgc
      241 attacaatgg ccttggtgca gctacttttg cattacaatg gccttggtgc agctactttt
      301 gcattacaat ggccttggtg actagttagg cgtgtacggg ccaacttttg tatacaaagt
      361 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
      421 aaataaaatc attatttgga gctctagagc tgcagggcgc agggcccgtg tctcaaaatc
      481 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
      541 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
      601 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
      661 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
      721 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
      781 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
      841 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
      901 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
      961 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     1021 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     1081 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcgccg gattcagtcg
     1141 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     1201 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     1261 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     1321 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     1381 aattggttaa ttggttgtaa caccggcaga gcattacgct gacttgacgg gacggcgcaa
     1441 gctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1621 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2161 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcca
     2221 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
     2281 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
     2341 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     2401 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
     2461 ccctactctc gcgttaacgc taccatggag ctc
//
