LOCUS       sequence-452339-        8579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a cancer stem cell reporter that responds 
            to presence of Sox2/Oct4 or their paralogs (green configuration).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Tang B, Raviv A, Esposito D, Flanders KC, Daniel C, Nghiem BT, 
            Garfield S, Lim L, Mannan P, Robles AI, Smith WI Jr, Zimmerberg J, 
            Ravin R, Wakefield LM
  TITLE     A flexible reporter system for direct observation and isolation of 
            cancer stem cells.
  JOURNAL   Stem Cell Reports. 2015 Jan 13;4(1):155-169. doi: 
            10.1016/j.stemcr.2014.11.002. Epub 2014 Dec 11.
  PUBMED    25497455
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.11.002"; journalName: "Stem Cell Reports"; 
            date: "2015-01-13- 13"; volume: "4"; issue: "1"; pages: "155-169"
COMMENT     Sequence Label: 8522-M01-663
FEATURES             Location/Qualifiers
     source          1..8579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    153..269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    303..323
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(568..588)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        587..625
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     622..646
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    707..731
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             734..1399
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             734..1399
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     protein_bind    complement(1536..1560)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1590..2178
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1643..1663)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2061..2072)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2192..2691
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2227..2250)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2624..2643
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2712..3311
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2712..2731)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3208..3228
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3449..3682
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3754..3875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3791..3810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3845..3864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3915..4050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4070..4089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4071..4089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4099..4116)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4099..4115)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4108..4130)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4257..4712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4344..4363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4554..4575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4738..4842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4843..4911,4912..5703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5061..5080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5874..6462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6363..6382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6616..6633
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6750..6771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6786..6791,6792..6809,6810..6816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6824..6840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6829..6851
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6848..6864
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6848..6864
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6883..6903
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6885..6903
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6931..7157
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7158..7338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7385..7510
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8003..8236
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8421..8465
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
       61 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
      121 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg
      181 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      241 caaaaacaaa ttacaaaatt caaaatttta tcgtccagtg tggtggaatt ctgcagatat
      301 cacaactttg tatagaaaag ttggccaaat tacaaaattc aaaattttat cgatcagcta
      361 cttttgcatt acaatggcct tggtgcagct acttttgcat tacaatggcc ttggtgcagc
      421 tacttttgca ttacaatggc cttggtggaa ttccagctac ttttgcatta caatggcctt
      481 ggtgcagcta cttttgcatt acaatggcct tggtgcagct acttttgcat tacaatggcc
      541 ttggtgacta gttaggcgtg tacgggccaa cttttgtata caaagtggta ggcgtgtacg
      601 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      661 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcacaa ctttgtacaa
      721 aaaagttggc accatgcccg ccatgaagat cgagtgccgc atcaccggca ccctgaacgg
      781 cgtggagttc gagctggtgg gcggcggaga gggcaccccc gagcagggcc gcatgaccaa
      841 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
      901 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
      961 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
     1021 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
     1081 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
     1141 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
     1201 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
     1261 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg
     1321 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa
     1381 gacccccatc gccttcgcca gatctcgaga tatcagccat ggcttcccgc cggcggtggc
     1441 ggcgcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca
     1501 ccctgcagcc tgtgcttctg ctaggatcaa tgtgtaatca actttcttgt acaaagttgt
     1561 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     1621 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1681 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1741 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1801 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1861 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     1921 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     1981 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     2041 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2101 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2161 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     2221 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     2281 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     2341 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     2401 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     2461 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     2521 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     2581 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     2641 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     2701 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     2761 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     2821 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     2881 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     2941 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     3001 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     3061 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     3121 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     3181 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     3241 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     3301 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     3361 atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     3421 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     3481 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3541 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3601 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3661 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     3721 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3841 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3901 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3961 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4021 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4081 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4141 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4201 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4261 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4321 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4381 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4441 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4501 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4561 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4681 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6001 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6541 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6601 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6661 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6721 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6781 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6841 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     6901 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     6961 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     7021 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7081 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7141 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7201 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7261 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7321 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7381 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     7441 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     7501 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7561 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7621 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7681 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7741 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7801 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     7861 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     7921 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     7981 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8041 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8101 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8161 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8221 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8281 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8341 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     8401 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     8461 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     8521 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaataga
//
