LOCUS       sequence-452346-        8670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLAG-tagged fluorescent proteins for 
            immunohistochemical detection in FFPE tissue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Sharma VP, Tang B, Wang Y, Duran CL, Karagiannis GS, Xue EA, 
            Entenberg D, Borriello L, Coste A, Eddy RJ, Kim G, Ye X, Jones JG, 
            Grunblatt E, Agi N, Roy S, Bandyopadhyaya G, Adler E, Surve CR, 
            Esposito D, Goswami S, Segall JE, Guo W, Condeelis JS, Wakefield LM,
            Oktay MH
  TITLE     Live tumor imaging shows macrophage induction and TMEM-mediated 
            enrichment of cancer stem cells during metastatic dissemination.
  JOURNAL   Nat Commun. 2021 Dec 15;12(1):7300. doi: 10.1038/s41467-021-27308-2.
  PUBMED    34911937
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-15"; volume: "12(1):7300. doi"; pages: " 
            10.1038/s41467-021-27308-2"
COMMENT     Sequence Label: 18065-M01-412
FEATURES             Location/Qualifiers
     source          1..8670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1941..1961
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(2206..2226)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2225..2263
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2260..2284
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2345..2369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2375..2440
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2441..3103
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             2441..3103
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     protein_bind    complement(3240..3264)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3278..3866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3331..3351)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3749..3760)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3917..3937)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3961..4171
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3983..4179
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4030..4165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4227..4274
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    complement(5317..5337)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             5406..5639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5711..5832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5748..5767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5802..5821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5872..6007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6040..6056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6040..6056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6053..6075)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6064..6080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6088..6094,6095..6112,6113..6118))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6133..6154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6271..6288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6442..7030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6522..6541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7201..7992,7993..8061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7824..7843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8062..8166)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8234..8252
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8290..8312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8412..8431
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8625..8647
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8639..8656
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8640..8656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgatgaattc ccgctcgaat caactttgta tagaaaagtt ggccaaatta caaaattcaa
     1981 aattttatcg atcagctact tttgcattac aatggccttg gtgcagctac ttttgcatta
     2041 caatggcctt ggtgcagcta cttttgcatt acaatggcct tggtggaatt ccagctactt
     2101 ttgcattaca atggccttgg tgcagctact tttgcattac aatggccttg gtgcagctac
     2161 ttttgcatta caatggcctt ggtgactagt taggcgtgta cgggccaact tttgtataca
     2221 aagtggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2281 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     2341 cagcacaact ttgtacaaaa aagttggcac catggactac aaagaccatg acggtgatta
     2401 taaggatcac gacatcgatt acaaggatga cgatgacaag cccgccatga agatcgagtg
     2461 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     2521 ccccgagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     2581 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     2641 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     2701 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     2761 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     2821 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     2881 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     2941 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     3001 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     3061 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatctc gagatatcag
     3121 ccatggcttc ccgccggcgg tggcggcgca ggatgatggc acgctgccca tgtcttgtgc
     3181 ccaggagagc gggatggacc gtcaccctgc agcctgtgct tctgctagga tcaatgtgta
     3241 atcaactttc ttgtacaaag ttgtgattcg actcgacaat caacctctgg attacaaaat
     3301 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3361 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3421 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3481 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3541 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3601 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3661 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     3721 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3781 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3841 gatctccctt tgggccgcct ccccgcctgg aattctgcag atatccggtt agtaatgagt
     3901 ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3961 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4081 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4141 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4201 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     4261 tagtataata cgacaaggtg aggaactaaa ccatgccacc tcctcgcctc ctcttcttcc
     4321 tcctcttcct cacccccatg gaagtcaggc ccgaggaacc tctagtggtg aaggtggaag
     4381 agggagataa cgctgtgctg cagtgcctca aggggacctc agatggcccc actcagcagc
     4441 tgacctggtc tcgggagtcc ccgcttaaac ccttcttaaa actcagcctg gggctgccag
     4501 gcctgggaat ccacatgagg cccctggcca tctggctttt catcttcaac gtctctcaac
     4561 agatgggggg cttctacctg tgccagccgg ggcccccctc tgagaaggcc tggcagcctg
     4621 gctggacagt caatgtggag ggcagcgggg agctgttccg gtggaatgtt tcggacctag
     4681 gtggcctggg ctgtggcctg aagaacaggt cctcagaggg ccccagctcc ccttccggga
     4741 agctcatgag ccccaagctg tatgtgtggg ccaaagaccg ccctgagatc tgggagggag
     4801 agcctccgtg tctcccaccg agggacagcc tgaaccagag cctcagccag gacctcacca
     4861 tggcccctgg ctccacactc tggctgtcct gtggggtacc ccctgactct gtgtccaggg
     4921 gccccctctc ctggacccat gtgcacccca aggggcctaa gtcattgctg agcctagagc
     4981 tgaaggacga tcgcccggcc agagatatgt gggtaatgga gacgggtctg ttgttgcccc
     5041 gggccacagc tcaagacgct ggaaagtatt attgtcaccg tggcaacctg accatgtcat
     5101 tccacctgga gatcactgct cggccagtac tatggcactg gctgctgagg actggtggct
     5161 ggaaggtctc agctgtgact ttggcttatc tgatcttctg cctgtgttcc cttgtgggca
     5221 ttcttcatct tcaaagagcc ctggtcctga ggaggaaaag aaagcgaatg actgacccca
     5281 ccaggagatt ctaagcacaa ttcgagctcg gtacctcaac tttattatac atagttgatc
     5341 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5401 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     5461 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5521 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5581 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5641 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5701 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5761 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5821 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5881 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5941 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6001 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     6061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8161 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8221 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8281 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8341 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     8401 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     8461 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     8521 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     8581 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     8641 taaaacgacg gccagtgcca agctgacgcg
//
