LOCUS       sequence-452357-        2357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae 
            depletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
  TITLE     
  JOURNAL   Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. 
            Epub 2024 Jul 24.
  PUBMED    39059674
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2024-09"; volume: "85:116-130. doi"; pages: " 
            10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24"
COMMENT     Sequence Label: pCOLA-Gent-ppiB
FEATURES             Location/Qualifiers
     source          1..2357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(181..816)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1677..1704
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1710..1800
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gaagtgccag acgatggaag gacgcaatga agctggagga ttggcgtggg aatcgtgctt
       61 ctgtctaagc aagaatgcct agcgtacagg gtgcactttg taacgatttg ggagtccaga
      121 gactcgctgt tttcgaaatt ctcggtcgct acgctccggg cgtgagacta tgcagcgcag
      181 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
      241 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      421 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      481 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      541 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      601 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttggtatctg
      661 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      721 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag
      781 gatctcaaga agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
      841 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
      901 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
      961 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgtgt gcgtaattgt
     1021 gctgatctct tatatagctg ctctcattat ctctctaccc tgaagtgact ctctcacctg
     1081 taaaaataat atctcacagg cttaatagtt tcttaataca aagcctgtaa aacgtcagga
     1141 taacttctgt gtaggaggat aatctatggt aacattccac actaaccacg gggacatcgt
     1201 tattaaaaca ttcgatgata aagctccgga aacagtcaag aactttctgg actattgtcg
     1261 cgaggggttc tacaataata caatcttcca ccgtgttatt aacgggttca tgatccaagg
     1321 cggagggttc gagccgggca tgaagcagaa agctactaaa gagccaatta agaacgaagc
     1381 aaacaatggt ctgaagaaca ctcgtggtac gcttgcgatg gcccgtaccc aagcgccaca
     1441 tagcgccacc gcccaattct ttattaatgt tgtggataat gattttctga acttctccgg
     1501 agaatcgtta cagggttggg gctactgcgt tttcgcagaa gtcgtcgacg gtatggatgt
     1561 cgtggataag atcaaaggag ttgcgacggg tcgtagcggt atgcaccaag acgtgccgaa
     1621 ggaggatgtg attatcgagt ctgtgactgt ttctgaataa ggatccgagt cacactggct
     1681 caccttcggg tgggcctttc tgcgtttatt gatcggcacg taagaggttc caactttcac
     1741 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1801 ggaaggaagt aaaaatgttg cgtagctcta acgatgtgac gcaacaaggt tcgcgtccaa
     1861 agacaaaatt gggaggcagt agcatgggga tcattcgcac ttgtcgcctg gggccagacc
     1921 aggtgaagtc aatgcgtgcg gctctggact tattcgggcg cgaatttgga gatgtagcca
     1981 cttactcaca gcaccaaccg gacagtgatt acttggggaa tttacttcgc agtaaaactt
     2041 ttatcgcttt ggccgctttc gaccaggagg ctgtagtagg tgcgttggca gcctatgttc
     2101 ttcctaaatt cgagcaaccg cgtagcgaaa tttacatcta tgatcttgca gtctccggcg
     2161 aacatcgccg tcaggggatc gccacagctt taatcaacct tttgaagcat gaggctaatg
     2221 cacttggagc gtacgtgatt tatgtgcagg ctgactacgg tgatgatcct gcagtcgctc
     2281 tgtacaccaa actgggtatc cgcgaggagg tcatgcactt tgatattgac ccgtctacgg
     2341 ctacctaata gaagctt
//
